Instituto Nacional Of Ciencia E Tecnologia Of Optica E Fotonica

São Carlos, Brazil

Instituto Nacional Of Ciencia E Tecnologia Of Optica E Fotonica

São Carlos, Brazil
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Pinheiro A.L.B.,Federal University of Bahia | Pinheiro A.L.B.,Parque Tecnológico São José dos Campos | Pinheiro A.L.B.,Instituto Nacional Of Ciencia E Tecnologia Of Optica E Fotonica | Soares L.G.P.,Federal University of Bahia | And 4 more authors.
Lasers in Medical Science | Year: 2012

We studied peaks of calcium hydroxyapatite (CHA) and protein and lipid CH groups in defects grafted with mineral trioxide aggregate (MTA) treated or not with LED irradiation, bone morphogenetic proteins and guided bone regeneration. A total of 90 rats were divided into ten groups each of which was subdivided into three subgroups (evaluated at 15, 21 and 30 days after surgery). Defects were irradiated with LED light (wavelength 850±10 nm) at 48-h intervals for 15 days. Raman readings were taken at the surface of the defects. There were no statistically significant differences in the CHA peaks among the nonirradiated defects at any of the experimental timepoints. On the other hand, there were significant differences between the defects filled with blood clot and the irradiated defects at all time-points (p<0.001, p=0.02, p<0.001). There were significant differences between the mean peak CHA in nonirradiated defects at all the experimental timepoints (p<0.01). The mean peak of the defects filled with blood clot was significantly different from that of the defects filled with MTA (p<0.001). There were significant differences between the defects filled with blood clot and the irradiated defects (p<0.001). The results of this study using Raman spectral analysis indicate that infrared LED light irradiation improves the deposition of CHA in healing bone grafted or not with MTA. © copy; Springer-Verlag London Ltd 2011.


Carvalho C.M.,Federal University of Bahia | Lacerda J.A.,Federal University of Bahia | Dos Santos Neto F.P.,Federal University of Bahia | De Castro I.C.V.,Federal University of Bahia | And 8 more authors.
Photomedicine and Laser Surgery | Year: 2011

Aim: The aim of this study was to evaluate, by light microscopy, the effects of laser phototherapy (LPT) at 780nm or a combination of 660 and 790nm, on the inflammatory process of the rat temporomandibular joint (TMJ) induced by carrageen. Background: Temporomandibular disorders (TMDs) are frequent in the population and generally present an inflammatory component. Previous studies have evidenced positive effects of laser phototherapy on TMDs. However, its mechanism of action on the inflammation of the TMJ is not known yet. Materials and Methods: Eighty-five Wistar rats were divided into 9 groups: G1, Saline; G2, Saline+LPT IR; G3, Saline+LPT IR+R; G4, Carrageenan; G5, Carrageenan+LPT IR; G6, Carrageenan+LPT IR+R; G7, previous LPT+Carrageenan; G8, previous LPT+carrageenan+LPT IR; and G9, previous LPT+carrageenan+LPT IR+R, and then subdivided in subgroups of 3 and 7 days. After animal death, specimens were taken, routinely cut and stained with HE, Sirius Red, and Toluidine Blue. Descriptive analysis of components of the TMJ was done. The synovial cell layers were counted. Results: Injection of saline did not produced inflammatory reaction and the irradiated groups did not present differences compared to nonirradiated ones. After carrageenan injection, intense inflammatory infiltration and synovial cell layers proliferation were observed. The infrared irradiated group presented less inflammation and less synovial cell layers number compared to other groups. Previous laser irradiation did not improve the results. Conclusion: It was concluded that the LPT presented positive effects on inflammatory infiltration reduction and accelerated the inflammation process, mainly with IR laser irradiation. The number of synovial cell layers was reduced on irradiated group. © Copyright 2011, Mary Ann Liebert, Inc. 2011.


De Sousa A.P.C.,Federal University of Bahia | De Aguiar Valenca Neto A.D.A.P.,Federal University of Bahia | Marchionni A.M.T.,Federal University of Bahia | De Araujo Ramos M.,Federal University of Bahia | And 7 more authors.
Photomedicine and Laser Surgery | Year: 2011

Objective: The aim of the present investigation was to evaluate transforming growth factor β (TGF-β) expression on cutaneous wounds in rodents treated or not treated with LED light. Background: TGF-β is a multifunctional cytokine that presents a central action during tissue repair. Although several studies both in vitro and in vivo have shown that LED phototherapy influences tissue repair, a full understanding of the mechanisms involved in its usage, such as in the modulation of some growth factors, remains unclear. Materials and Methods: Under general anesthesia, 24 young adult male Wistar rats weighing 200-250 g had one excisional wound created on the dorsum of each, and were randomly distributed into two groups: G0 (Control) and G1 (LED, λ700 ± 20 nm, 16 mW, SAEF = 5 J/cm2, Illuminated Area = 2 cm2, 8 mWcm2, 626 s) Each group was subdivided into three subgroups according to the animal death timing (2, 4, and 6 days). LED phototherapy started immediately after surgery and was repeated every other day during the experimental time. Following animal death, specimens were removed, routinely processed to wax, cut and immunomarked with polyclonal anti-TGF-β, and underwent histological analysis by light microscopy. The mean area of expression of each group was calculated. The data were statistically analyzed using ANOVA and Tukey's test. Results: The area of the expression of TGF-β on LED-irradiated animals was significantly smaller than on controls at day 2 (p = 0.013). No significant difference was found at later times. It is concluded that the use of LED light, at these specific parameters, caused an inhibition of the expression of TGF-β at an early stage of the healing process. © Copyright 2011, Mary Ann Liebert, Inc.


Gama S.K.C.,Federal University of Bahia | Habib F.A.L.,Federal University of Bahia | De Carvalho Monteiro J.S.,Federal University of Bahia | Paraguassu G.M.,Federal University of Bahia | And 5 more authors.
Photomedicine and Laser Surgery | Year: 2010

Objectives: The aim of this research was to investigate the influence of low-power laser on tooth movement in rats. Background: Tooth movement is closely related to the process of bone remodeling. The biologic result, with the application of a force to the tooth, is bone absorption on the pressure side and neoformation on the traction side of the alveolar bone. The laser photobiomodulation is capable of providing an increase in cellular metabolism, blood flow, and lymphatic drainage. Methods: Thirty young-adult male Wistar rats weighing between 250 and 300g were divided into two groups, control and experimental, containing 15 animals each. The animals received orthodontic devices calibrated to release a force of 40g/F, with the purpose of moving the first upper molar mesially. Low-intensity laser, wavelength 790nm, was used in the experimental group; the dose was 4.5J/cm2 per point, mesial and distal, on the palatal side, 11J/cm2 on the buccal side, and this procedure was repeated every 48h, totaling nine applications. The active movement was clinically evaluated after 7, 13, and 19 days. Results and conclusion: The results showed no statistically significant difference, p=0.079 (T0-T7), p=0.597 (T7-T13), and p=0.550 (T13-T19) between the laser and control groups on the amount of tooth movement in the different times evaluated. It may be concluded that laser phototherapy, with the parameters in the present study, did not significantly increase the amount of tooth displacement during induced orthodontic movement in rodents. © Copyright 2010, Mary Ann Liebert, Inc. 2010.


Habib F.A.L.,Federal University of Bahia | Gama S.K.C.,Federal University of Bahia | Ramalho L.M.P.,Federal University of Bahia | Cangussu M.C.T.,Federal University of Bahia | And 6 more authors.
Photomedicine and Laser Surgery | Year: 2010

Objective: The aim of this study was to assess by light microscopy changes in alveolar bone during orthodontic movement in rats. Background: Orthodontic movement causes both removal and deposition of bone tissue. The use of laser phototherapy (LPT) is considered an enhancement factor for bone repair. Methods: Thirty Wistar rats were divided into two groups (n=15) and subdivided according to animal death (7,13, and 19 days). Half of the animals in each group were treated with LPT during orthodontic movement. After animal death, specimens were processed and underwent histological and semi-quantitative analyses (HE and Sirius red). Results: LPT-irradiated specimens showed significantly higher numbers of osteoclasts when compared with controls at both 7 (p=0.015) and 19 (p=0.007) days, as well as significant increases in the number of osteoblasts (p=0.015) between days 7 and 13. The amount of collagen matrix was significantly reduced between days 7 and 13 at both pressure and tension sites in controls (p=0.015) but not in LPT-treated animals. LPT-treated subjects showed significantly greater deposition of collagen matrix at the pressure site at both the thirteenth (p=0.007) and nineteenth days (p=0.001). At the tension site, a significant increase in the amount of collagen matrix was observed in non-irradiated specimens (p=0.048) between days 7 and 19. Conclusions: LPT caused significant histological changes in the alveolar bone during induced tooth movement, including alterations in the number of both osteoclasts and osteoblasts and in collagen deposition in both pressure and tension areas. Copyright 2010, Mary Ann Liebert, Inc.


De Sousa A.P.C.,Federal University of Bahia | Santos J.N.,Federal University of Bahia | Dos Reis J.A.,Federal University of Bahia | Ramos T.A.,Federal University of Bahia | And 5 more authors.
Photomedicine and Laser Surgery | Year: 2010

Aim: The aim of the present investigation was to evaluate histologically fibroblastic proliferation on dorsal cutaneous wounds in a rodent model treated or not with light-emitting diodes (LEDs) of three wavelengths. Background: Fibroblasts secrete substances essential for wound healing. There are few reports of LED phototherapy on fibroblast proliferation, mainly in vivo. Animals and Methods: Following approval by the Animal Experimentation Committee of the School of Dentistry of the Federal University of Bahia, we obtained 16 young adult male Wistar rats weighing between 200 and 250g. Under general anesthesia, one excisional wound was created on the dorsum of each animal; they were then randomly distributed into four groups of four animals each: G0, untreated control; G1, red LED (700±20nm, 15mW, 10J/cm2); G2, green LED (530±20nm, 8mW, 10J/cm2); and G3, blue LED (460±20nm, 22mW, 10J/cm2). The irradiation started immediately after surgery and was repeated every other day for 7 days. Animals were killed 8 days after surgery. The specimens were removed, routinely processed to wax, cut, and stained with hematoxylin/eosin (HE). Fibroblasts were scored by measuring the percentage of these cells occupying the area corresponding to wound healing on stained sections. Results: The quantitative results showed that red LED (700±20nm) and green LED (530±20nm) showed a significant increase in fibroblast numbers (p<0.01 and p=0.02) when compared with the control group. Conclusion: The use of green and red LED light is effective in increasing fibroblastic proliferation on rodents. Copyright 2010, Mary Ann Liebert, Inc.


Pinheiro A.L.B.,Federal University of Bahia | Pinheiro A.L.B.,University Camilo Castelo Branco | Pinheiro A.L.B.,Instituto Nacional Of Ciencia E Tecnologia Of Optica E Fotonica | Lopes C.B.,Harvard University | And 5 more authors.
Photomedicine and Laser Surgery | Year: 2010

Objectives: We aimed to assess through Raman spectroscopy and fluorescence the levels of calcium hydroxyapatite (CHA) and lipids and proteins in complete fractures treated with internal rigid fixation (IRF) treated or not with laser phototherapy (LPT) and associated or not with bone morphogenetic proteins (BMPs) and guided bone regeneration (GBR). Background: Fractures have different etiologies and treatments and may be associated with bone losses. LPT has been shown to improve bone healing. Methods: Tibial fractures were created on 15 animals and divided into five groups. LPT started immediately after surgery, repeated at 48-h tervals. Animal death occurred after 30 days. Results: Raman spectroscopy and fluorescence were performed at the surface. Fluorescence data of group IRF + LPT + Biomaterial showed similar readings to those of the group IRF-no bone loss. Significant differences were seen between groups IRF + LPT + Biomaterial and IRF + LPT; IRF + LPT + Biomaterial; and IRF + Biomaterial; and between IRF + LPT + Biomaterial and IRF. CH groups of lipids and proteins readings showed decreased levels of organic components in subjects treated with the association of LPT, biomaterial, and GBR. Pearson correlation showed that luorescence readings of both CHA and CH groups of lipids and proteins correlated atively with the Raman data. Conclusions: The use of both methods indicates that the use of the omaterials associated with infrared LPT resulted in a more-advanced and higher quality of bone repair in fractures treated with miniplates and that the DIAGNOdent may be used to perform optical biopsy on bone. © 2010, Mary Ann Liebert, Inc.


Pinheiro A.L.B.,Federal University of Bahia | Pinheiro A.L.B.,Parque Tecnológico São José dos Campos | Pinheiro A.L.B.,Instituto Nacional Of Ciencia E Tecnologia Of Optica E Fotonica | Santos N.R.S.,Federal University of Bahia | And 7 more authors.
Lasers in Medical Science | Year: 2013

The aim of the present study was to assess, by Raman spectroscopy and laser fluorescence, the repair of surgical fractures fixed with wire osteosynthesis treated or not with infrared laser (λ780 nm, 50 mW, 4 × 4 J/cm 2 = 16 J/cm2, φ = 0.5 cm2, CW) associated or not to the use of hydroxyapatite and guided bone regeneration. Surgical tibial fractures were created under general anesthesia on 15 rabbits that were divided into five groups, maintained on individual cages, at day/night cycle, fed with solid laboratory pelted diet, and had water ad libitum. The fractures in groups II, III, IV, and V were fixed with wires. Animals in groups III and V were grafted with hydroxyapatite (HA) and guided bone regeneration (GBR) technique used. Animals in groups IV and V were irradiated at every other day during 2 weeks (4 × 4 J/cm2, 16 J/cm2 = 112 J/cm2). Observation time was that of 30 days. After animal death, specimens were taken and kept in liquid nitrogen and used for Raman spectroscopy. The Raman results showed basal readings of 1,234.38 ± 220. Groups WO + B + L showed higher readings (1,680.22 ± 822) and group WO + B the lowest (501.425 ± 328). Fluorescence data showed basal readings of 5.83333 ± 0.7. Groups WO showed higher readings (6.91667 ± 0.9) and group WO + B + L the lowest (1.66667 ± 0.5). There were significant differences between groups on both cases (p < 0.05). Pearson correlation was negative and significant (R 2 = -0.60; p < 0.001), and it was indicative that, when the Raman peaks of calcium hydroxyapatite (CHA) are increased, the level of fluorescence is reduced. It is concluded that the use of near-infrared lasertherapy associated to HA graft and GBR was effective in improving bone healing on fractured bones as a result of the increasing deposition of CHA measured by Raman spectroscopy and decrease of the organic components as shown by the fluorescence readings. © 2012 Springer-Verlag London Ltd.


Pinheiro A.L.B.,Federal University of Bahia | Pinheiro A.L.B.,University Camilo Castelo Branco | Pinheiro A.L.B.,Instituto Nacional Of Ciencia E Tecnologia Of Optica E Fotonica | Santos N.R.S.,Federal University of Bahia | And 7 more authors.
Lasers in Medical Science | Year: 2013

Abstracts: The aim of the present study was to assess, by Raman spectroscopy, the repair of surgical fractures fixed with internal rigid fixation (IRF) treated or not with IR laser (λ780 nm, 50 mW, 4 × 4 J/cm2 = 16 J/cm2, = 0.5 cm2, CW) associated or not to the use of hydroxyapatite and guided bone regeneration (GBR). Surgical tibial fractures were created under general anesthesia on 15 rabbits that were divided into five groups, maintained on individual cages, at day/night cycle, fed with solid laboratory pelted diet and had water ad libitum. The fractures in groups II, III, IV and V were fixed with miniplates. Animals in groups III and V were grafted with hydroxyapatite and GBR technique used. Animals in groups IV and V were irradiated at every other day during 2 weeks (4 × 4 J/cm 2, 16 J/cm2 = 112 J/cm2). Observation time was that of 30 days. After animal death, specimens were taken and kept in liquid nitrogen and used for Raman spectroscopy. Raman spectroscopy showed significant differences between groups (p < 0.001). Basal readings showed mean value of 1,234 ± 220.1. Group internal rigid fixation + biomaterial + laser showed higher readings (3,521 ± 2,670) and group internal rigid fixation + biomaterial the lowest (212.2 ± 119.8). In conclusion, the results of the present investigation are important clinically as spectral analysis of bone component evidenced increased levels of CHA on fractured sites by using the association of laser light to a ceramic graft. © 2012 Springer-Verlag London Ltd.


Pinheiro A.L.B.,Federal University of Bahia | Pinheiro A.L.B.,Parque Tecnológico São José dos Campos | Pinheiro A.L.B.,Instituto Nacional Of Ciencia E Tecnologia Of Optica E Fotonica | Aciole G.T.S.,Federal University of Bahia | And 3 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2010

We have used Raman analysis to assess bone healing on different models. Benefits on the isolated or combined use of mineral trioxide aggregate, bone morphogenetic proteins, guided bone regeneration and laser on bone repair have been reported, but not their combination. We studied peaks of hydroxyapatite and CH groups on defects grafted with MTA, treated or not with laser, BMPs, and GBR. Ninety rats were divided in 10 groups each, subdivided into three subgroups. Laser (λ850 nm) was applied at every other day for 2 weeks. Raman readings were taken at the surface of the defect. Statistical analysis (CHA) showed significant differences between all groups (p = 0.001) and between Group II and all other (p < 0.001), but not with Group X (p = 0.09). At day 21 differences were seen between all groups (p = 0.031) and between Groups VIII and X when compared with Groups VI (p = 0.03), V (p < 0.001), IV (p < 0.001), and IX (p = 0.04). At the end of the experimental period no significant differences were seen. On regards CH, significant differences were seen at the 15th day (p = 0.002) and between Group II and all other groups (p < 0.0001) but not with control. Advanced maturation on irradiated bone is because of increased secretion of calcium hydroxyapatite (CHA) that is indicative of greater calcification and resistance of the bone. We conclude that the association of the MTA with laser phototherapy (LPT) and/or not with GBR resulted in a better bone repair. The use of the MTA associated to IR LPT resulted in a more advanced and quality bone repair. © 2010 Wiley Periodicals, Inc.

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