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Pinheiro A.L.B.,Federal University of Bahia | Pinheiro A.L.B.,University Camilo Castelo Branco | Pinheiro A.L.B.,Instituto Nacional Of Ciencia E Tecnologia Of Optica E Fotonica | Lopes C.B.,Harvard University | And 5 more authors.
Photomedicine and Laser Surgery | Year: 2010

Objectives: We aimed to assess through Raman spectroscopy and fluorescence the levels of calcium hydroxyapatite (CHA) and lipids and proteins in complete fractures treated with internal rigid fixation (IRF) treated or not with laser phototherapy (LPT) and associated or not with bone morphogenetic proteins (BMPs) and guided bone regeneration (GBR). Background: Fractures have different etiologies and treatments and may be associated with bone losses. LPT has been shown to improve bone healing. Methods: Tibial fractures were created on 15 animals and divided into five groups. LPT started immediately after surgery, repeated at 48-h tervals. Animal death occurred after 30 days. Results: Raman spectroscopy and fluorescence were performed at the surface. Fluorescence data of group IRF + LPT + Biomaterial showed similar readings to those of the group IRF-no bone loss. Significant differences were seen between groups IRF + LPT + Biomaterial and IRF + LPT; IRF + LPT + Biomaterial; and IRF + Biomaterial; and between IRF + LPT + Biomaterial and IRF. CH groups of lipids and proteins readings showed decreased levels of organic components in subjects treated with the association of LPT, biomaterial, and GBR. Pearson correlation showed that luorescence readings of both CHA and CH groups of lipids and proteins correlated atively with the Raman data. Conclusions: The use of both methods indicates that the use of the omaterials associated with infrared LPT resulted in a more-advanced and higher quality of bone repair in fractures treated with miniplates and that the DIAGNOdent may be used to perform optical biopsy on bone. © 2010, Mary Ann Liebert, Inc.

Silva Santos N.R.,Federal University of Bahia | Aciole G.T.S.,Federal University of Bahia | Marchionni A.M.T.,Federal University of Bahia | Soares L.G.P.,Federal University of Bahia | And 4 more authors.
Photomedicine and Laser Surgery | Year: 2010

Objectives: The aim of this work is to report some cases of surgical removal of hyperkeratotic lesions of the oral cavity with the CO2 laser. Background: Hyperkeratosis is an abnormal thickening of the stratum corneum caused by increased deposition of keratin, and its histopathologic features show wide variations. These changes are significant because they determine different biologic behavior. Several techniques are used to treat these lesions, including scalpel incision, electrosurgery, cryosurgery, photodynamic therapy, and some drugs. The use of surgical lasers has been proposed as an effective way of treating such lesions safely. The CO2 laser is the most used laser on the oral cavity because of its affinity for water and high absorption by the oral mucosa. Several benefits of the use of the CO2 laser are reported in the literature in regard to surgical procedures carried out on the oral cavity. Patients and Methods: All patients had histopathologic diagnosis of hyperkeratosis and mild epithelial dysplasia and were routinely prepared for surgery under local anesthesia. The surgical procedures were carried out by using a CO2 laser (Sharplan 20 C; Laser Industries, Tel Aviv, Israel, λ10,600nm, φ∼2mm, CW/RSP). The beam was focused to delimit each lesion, and then lesions were excised, and the removed specimens were sent for histopathology. At the end of the surgery, the beam was used in a defocused manner to promote better hemostasis. Neither sutures nor dressings were used after the surgery. No medication but mouthwashes was prescribed to all patients in the postoperative period. Conclusions: The use of the CO2 laser does not reduce the risk of relapses of the lesion, but it is an easy-to-use technique and results in both a quick surgical procedure and trouble-free postoperative period and may be safely used in dental practice. © 2010, Mary Ann Liebert, Inc.

De Sousa A.P.C.,Federal University of Bahia | De Aguiar Valenca Neto A.D.A.P.,Federal University of Bahia | Marchionni A.M.T.,Federal University of Bahia | De Araujo Ramos M.,Federal University of Bahia | And 7 more authors.
Photomedicine and Laser Surgery | Year: 2011

Objective: The aim of the present investigation was to evaluate transforming growth factor β (TGF-β) expression on cutaneous wounds in rodents treated or not treated with LED light. Background: TGF-β is a multifunctional cytokine that presents a central action during tissue repair. Although several studies both in vitro and in vivo have shown that LED phototherapy influences tissue repair, a full understanding of the mechanisms involved in its usage, such as in the modulation of some growth factors, remains unclear. Materials and Methods: Under general anesthesia, 24 young adult male Wistar rats weighing 200-250 g had one excisional wound created on the dorsum of each, and were randomly distributed into two groups: G0 (Control) and G1 (LED, λ700 ± 20 nm, 16 mW, SAEF = 5 J/cm2, Illuminated Area = 2 cm2, 8 mWcm2, 626 s) Each group was subdivided into three subgroups according to the animal death timing (2, 4, and 6 days). LED phototherapy started immediately after surgery and was repeated every other day during the experimental time. Following animal death, specimens were removed, routinely processed to wax, cut and immunomarked with polyclonal anti-TGF-β, and underwent histological analysis by light microscopy. The mean area of expression of each group was calculated. The data were statistically analyzed using ANOVA and Tukey's test. Results: The area of the expression of TGF-β on LED-irradiated animals was significantly smaller than on controls at day 2 (p = 0.013). No significant difference was found at later times. It is concluded that the use of LED light, at these specific parameters, caused an inhibition of the expression of TGF-β at an early stage of the healing process. © Copyright 2011, Mary Ann Liebert, Inc.

Carvalho C.M.,Federal University of Bahia | Lacerda J.A.,Federal University of Bahia | Dos Santos Neto F.P.,Federal University of Bahia | De Castro I.C.V.,Federal University of Bahia | And 8 more authors.
Photomedicine and Laser Surgery | Year: 2011

Aim: The aim of this study was to evaluate, by light microscopy, the effects of laser phototherapy (LPT) at 780nm or a combination of 660 and 790nm, on the inflammatory process of the rat temporomandibular joint (TMJ) induced by carrageen. Background: Temporomandibular disorders (TMDs) are frequent in the population and generally present an inflammatory component. Previous studies have evidenced positive effects of laser phototherapy on TMDs. However, its mechanism of action on the inflammation of the TMJ is not known yet. Materials and Methods: Eighty-five Wistar rats were divided into 9 groups: G1, Saline; G2, Saline+LPT IR; G3, Saline+LPT IR+R; G4, Carrageenan; G5, Carrageenan+LPT IR; G6, Carrageenan+LPT IR+R; G7, previous LPT+Carrageenan; G8, previous LPT+carrageenan+LPT IR; and G9, previous LPT+carrageenan+LPT IR+R, and then subdivided in subgroups of 3 and 7 days. After animal death, specimens were taken, routinely cut and stained with HE, Sirius Red, and Toluidine Blue. Descriptive analysis of components of the TMJ was done. The synovial cell layers were counted. Results: Injection of saline did not produced inflammatory reaction and the irradiated groups did not present differences compared to nonirradiated ones. After carrageenan injection, intense inflammatory infiltration and synovial cell layers proliferation were observed. The infrared irradiated group presented less inflammation and less synovial cell layers number compared to other groups. Previous laser irradiation did not improve the results. Conclusion: It was concluded that the LPT presented positive effects on inflammatory infiltration reduction and accelerated the inflammation process, mainly with IR laser irradiation. The number of synovial cell layers was reduced on irradiated group. © Copyright 2011, Mary Ann Liebert, Inc. 2011.

Habib F.A.L.,Federal University of Bahia | Gama S.K.C.,Federal University of Bahia | Ramalho L.M.P.,Federal University of Bahia | Cangussu M.C.T.,Federal University of Bahia | And 6 more authors.
Photomedicine and Laser Surgery | Year: 2010

Objective: The aim of this study was to assess by light microscopy changes in alveolar bone during orthodontic movement in rats. Background: Orthodontic movement causes both removal and deposition of bone tissue. The use of laser phototherapy (LPT) is considered an enhancement factor for bone repair. Methods: Thirty Wistar rats were divided into two groups (n=15) and subdivided according to animal death (7,13, and 19 days). Half of the animals in each group were treated with LPT during orthodontic movement. After animal death, specimens were processed and underwent histological and semi-quantitative analyses (HE and Sirius red). Results: LPT-irradiated specimens showed significantly higher numbers of osteoclasts when compared with controls at both 7 (p=0.015) and 19 (p=0.007) days, as well as significant increases in the number of osteoblasts (p=0.015) between days 7 and 13. The amount of collagen matrix was significantly reduced between days 7 and 13 at both pressure and tension sites in controls (p=0.015) but not in LPT-treated animals. LPT-treated subjects showed significantly greater deposition of collagen matrix at the pressure site at both the thirteenth (p=0.007) and nineteenth days (p=0.001). At the tension site, a significant increase in the amount of collagen matrix was observed in non-irradiated specimens (p=0.048) between days 7 and 19. Conclusions: LPT caused significant histological changes in the alveolar bone during induced tooth movement, including alterations in the number of both osteoclasts and osteoblasts and in collagen deposition in both pressure and tension areas. Copyright 2010, Mary Ann Liebert, Inc.

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