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Blaser R.E.,University of San Diego | Rosemberg D.B.,Federal University of Rio Grande do Sul | Rosemberg D.B.,Instituto Nacional Of Ciencia E Tecnologia Em Excitotoxicidade E Neuroprotecao Inct En
PLoS ONE | Year: 2012

The effects of wall color stimuli on diving, and the effects of depth stimuli on scototaxis, were assessed in zebrafish. Three groups of fish were confined to a black, a white, or a transparent tank, and tested for depth preference. Two groups of fish were confined to a deep or a shallow tank, and tested for black-white preference. As predicted, fish preferred the deep half of a split-tank over the shallow half, and preferred the black half of a black/white tank over the white half. Results indicated that the tank wall color significantly affected depth preference, with the transparent tank producing the strongest depth preference and the black tank producing the weakest preference. Tank depth, however, did not significantly affect color preference. Additionally, wall color significantly affected shuttling and immobility, while depth significantly affected shuttling and thigmotaxis. These results are consistent with previous indications that the diving response and scototaxis may reflect dissociable mechanisms of behavior. We conclude that the two tests are complementary rather than interchangeable, and that further research on the motivational systems underlying behavior in each of the two tests is needed. © 2012 Blaser, Rosemberg. Source

Piato A.L.,Grande Rio University | Rosemberg D.B.,Federal University of Rio Grande do Sul | Capiotti K.M.,Grande Rio University | Capiotti K.M.,Instituto Nacional Of Ciencia E Tecnologia Translacional Em Medicina Inct Tm | And 11 more authors.
Neurochemical Research | Year: 2011

Despite the extensive knowledge about the effects of acute restraint stress (ARS) in rodents, zebrafish research is still elementary in this field, and the consequences of stress on purinergic system are unclear. Therefore, we evaluated the effects of ARS on behavior, biochemical, and molecular parameters in zebrafish brain. Animals were submitted to a 90 min ARS protocol and tested for anxiety levels, exploratory behavior, and memory performance. Furthermore, we analyzed ectonucleotidase and adenosine deaminase activities and their gene expression profile, as well as transcription of adenosinereceptors. ARS increased anxiety, but did not impair locomotion or cognition. ARS significantly increased ATP hydrolysis, decreased cytosolic ADA activity, and changed the entpd and adora gene expression. In conclusion, ARS disturbed zebrafish behavior, and we hypothesize that the augmentation in adenosine-mediated signaling may be a strategy to reestablish homeostasis and normal behavior after a stressful event. © Springer Science+Business Media, LLC 2011. Source

Menezes F.P.,Grande Rio University | Rico E.P.,Federal University of Rio Grande do Sul | Rico E.P.,Instituto Nacional Of Ciencia E Tecnologia Em Excitotoxicidade E Neuroprotecao Inct En | Da Silva R.S.,Grande Rio University | Da Silva R.S.,Instituto Nacional Of Ciencia E Tecnologia Translacional Em Medicina Inct Tm
Progress in Neuro-Psychopharmacology and Biological Psychiatry | Year: 2014

During brain development, the electrical disturbance promoted by a seizure can have several consequences, because it can disturb a set of steps extremely regulated needed to the correct brain maturation. Animal modeling of seizure is invaluable to contribute to the mechanistic understanding of punctual seizure event, and those that triggered in an immature neural network could alter the mature brain physiology. In the present study we observed that the exposure to kainic acid diluted directly in water of zebrafish decreased the locomotor activity at 7. days post-fertilization (dpf) animals and increased at 15. dpf, despite the absence of more specific seizure features. Pre-exposure to kainic acid (500. μM) diluted in water at 7. dpf animals reduced the susceptibility to a second exposure 2. months later by intraperitoneal injection. The current data suggest that these different responses are associated with neuronal maturation process and open a question about the window of development that are crucial to long lasting effects related to seizure in this animal model. © 2014 Elsevier Inc. Source

Zenki K.C.,Federal University of Rio Grande do Sul | Mussulini B.H.M.,Federal University of Rio Grande do Sul | Rico E.P.,Federal University of Rio Grande do Sul | Rico E.P.,Instituto Nacional Of Ciencia E Tecnologia Em Excitotoxicidade E Neuroprotecao Inct En | And 5 more authors.
Toxicology in Vitro | Year: 2014

Ethanol (EtOH) and its metabolite, acetaldehyde (ALD), induce deleterious effects on central nervous system (CNS). Here we investigate the in vitro toxicity of EtOH and ALD (concentrations of 0.25%, 0.5%, and 1%) in zebrafish brain structures [telencephalon (TE), opticum tectum (OT), and cerebellum (CE)] by measuring the functionality of glutamate transporters, MTT reduction, and extracellular LDH activity. Both molecules decreased the activity of the Na+-dependent glutamate transporters in all brain structures. The strongest glutamate uptake inhibition after EtOH exposure was 58% (TE-1%), and after ALD, 91% (CE-1%). The results of MTT assay and LDH released demonstrated that the actions of EtOH and its metabolite are concentration and structure-dependent, in which ALD was more toxic than EtOH. In summary, our findings demonstrate a differential toxicity in vitro of EtOH and ALD in zebrafish brain structures, which can involve changes on glutamatergic parameters. We suggest that this species may be an interesting model for assessing the toxicological actions of alcohol and its metabolite in CNS. © 2014 Elsevier Ltd. Source

Maximino C.,Federal University of Para | Maximino C.,Zebrafish Neuroscience Research Consortium | Benzecry R.,Federal University of Para | Matos Oliveira K.R.,Federal University of Para | And 10 more authors.
Behaviour | Year: 2012

The recent introduction of tasks to assess the behavior of zebrafish in novel and/or aversive environments has spurred great interest, prompting attempts to determine which constructs are modeled by these tasks (e.g., fear, anxiety, or some other construct). A review of the pharmacological and behavioral experiments indicates that not all behavioral testing models are equivalent. A more precise understanding of the parameters that influence task performance affords a wider selection of experimental procedures for investigating a particular construct, and also provides tools for differentiating the various constructs that may ultimately be of interest. In this review we will more closely examine two behavioral assays commonly used to measure the construct of 'anxiety' in adult zebrafish, with the conclusion that they do not both appear to be measuring a single underlying state. © 2012 Koninklijke Brill NV, Leiden. Source

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