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Victorio C.P.,Federal University of Rio de Janeiro | Victorio C.P.,University Estadual da Zona Oeste | Arruda R.D.C.D.O.,Rio de Janeiro State Federal University | Riehl C.A.S.,Federal University of Rio de Janeiro | Lage C.L.S.,Instituto Nacional da Propriedade Industrial INPI
Natural Product Research | Year: 2011

Plant leaves are commonly used in folk medicine and food industry. Their volatile composition is an important determinant in such applications. However, to properly assess the quality of volatiles, proper analytic tools must be utilised. Accordingly, the static headspace technique was used to evaluate the main volatiles emitted from in vitro-grown Alpinia zerumbet plants cultured with indole-3-acetic acid, thidiazuron, benzyladenine or kinetin, under standard physical conditions, as compared to those of field-grown donor plants. Although the leaf aroma of the donor plants was found to be a complex mixture, mainly consisting of sabinene, and -terpinene, 1,8-cineole and caryophyllene, volatile analyses from most of the in vitro samples only revealed the presence of sabinene and caryophyllene. Many alkanes were found in the aromas after treating plantlets with cytokinins. Histochemical analysis of leaf sections was also carried out. Secretory cells found in the epidermis and mesophyll showed a strong positive reaction to lipophilic compounds using Oil red and Nile blue reagents. These findings demonstrated how in vitro conditions may alter the quality of volatiles in micropropagation systems, while leaf anatomy analysis revealed a large quantity of oil cells in the mesophyll as a constant feature responsible for the production of volatile compounds in both donor and in vitro-grown plants. © 2011 Taylor & Francis.


The appellations of origin and indication of source are species of geographical indication (GI) provided by the Brazilian industrial property law that could be seen as one of the ways of mobilization and recovering of the rural territories adding value to local products with differentiated quality. This study aimed to characterize the stages of the construction of the regulations of use of GI, the geographical area delimitation, the evidence of reputation and the relationship between product and geographical environment of GIs in Brazil. The survey research was the methodology adopted conducted through semi-structured questionnaires. The results showed that despite the shortcomings of the Brazilian legislation on GIs, several organs has enabled the construction process of the Brazilian GIs acting decisively on the elaboration steps of the regulation of use (universities, NGOs, INPI), on the area delimitation (universities, Embrapa, Emater, IMA), on the evidence of reputation (SEBRAE) and on the relationship between geographical environment and quality (universities). The proof of the influence of the geographical environment on the product qualitative characteristics is currently the main obstacle to the development of appellations of origin in the country.


De Melo A.A.,Instituto Federal Of Educacao | Ribeiro G.L.,Instituto Federal Of Educacao | Martinez M.E.M.,Instituto Nacional da Propriedade Industrial INPI | Dos Reis P.C.,Instituto Nacional da Propriedade Industrial INPI
Proceedings - 2013 Symposium on Computing and Automation for Offshore Shipbuilding, NAVCOMP 2013 | Year: 2013

In order to evaluate the technologies related to underwater sensor networks were used two commercial software: Pat BaseMR (Minesoft MR) and Matheo Patent XEMR. These technologies are essential to collect oceanographic data, with various applications, e.g., seismic predictions, offshore oil and gas, control of water quality and studies of the interactions between the oceans and atmosphere. There were selected 85 patent documents related to underwater sensor networks between 2000 and 2011. The temporal evolution revealed a strong growth from 2006. Further, it was observed that the main offices where the patent documents have been deposited are: (a) of China (SIPO), (b) of South Korea (KIPO), and (c) of the United States (USPTO). © 2013 IEEE.


Da Silva D.,Federal University of Rio de Janeiro | Casanova L.M.,Federal University of Rio de Janeiro | Marcondes M.C.,Federal University of Rio de Janeiro | Espindola-Netto J.M.,Federal University of Rio de Janeiro | And 6 more authors.
IUBMB Life | Year: 2014

The aim of this study was to evaluate the antidiabetic potential of a leaf extract and flavonoids from Sedum dendroideum (SD). Additionally, our goals were to establish a possible structure/activity relationship between these flavonoids and to assess the most active flavonoid on the glycolytic enzyme 6-phosphofructo-1-kinase (PFK). SD juice (LJ), a flavonoid-rich fraction (BF), and separately five flavonoids were evaluated intraperitoneally for their acute hypoglycemic activity in normal and streptozotocin-induced diabetic mice. First, the major flavonoids kaempferol 3,7-dirhamnoside or kaempferitrin (1), kaempferol 3-glucoside-7-rhamnoside (2), and kaempferol 3-neohesperidoside-7- rhamnoside (3) were tested. Then, the monoglycosides kaempferol 7-rhamnoside (5) and kaempferol 3-rhamnoside (6) were assayed to establish their structure/activity relationship. The effect of 1 on PFK was evaluated in skeletal muscle, liver, and adipose tissue from treated mice. LJ (400 mg/kg), BF (40 mg/kg), and flavonoid 1 (4 mg/kg) reduced glycemia in diabetic mice (120 min) by 52, 53, and 61%, respectively. Flavonoids 2, 3, 5, and 6 were inactive or showed little activity, suggesting that the two rhamnosyl moieties in kaempferitrin are important requirements. Kaempferitrin enhanced the PFK activity chiefly in hepatic tissue, suggesting that it is able to stimulate tissue glucose utilization. This result is confirmed testing kaempferitrin on C2C12 cell line, where it enhanced glucose consumption, lactate production, and the key regulatory glycolytic enzymes. The hypoglycemic activity of kaempferitrin depends on the presence of both rhamnosyl residues in the flavonoid structure when intraperitoneally administered. Our findings show for the first time that a flavonoid is capable of stimulating PFK in a model of diabetes and that kaempferitrin stimulates glucose-metabolizing enzymes. This study contributes to the knowledge of the mechanisms by which this flavonoid exerts its hypoglycemic activity. © 2014 International Union of Biochemistry and Molecular Biology.


Labouriau F.C.,Instituto Nacional da Propriedade Industrial INPI | Naveiro R.M.,Federal University of Rio de Janeiro
21st International Conference on Production Research: Innovation in Product and Production, ICPR 2011 - Conference Proceedings | Year: 2011

This paper is focused on product development planning and the growing role that intellectual property is assuming in the technological strategies of the companies. Planning is one of the main phases of the design process as its interface with the enterprise business strategies is a driver for the definition of the technological strategies that will guide the search and the selection of ideas for product development. Powerful methodologies based on the patent system, some like TRIZ, have been used to obtain relevant information from the system thus fomenting new ideas for evaluation and selection, mapping the technology evolution or supporting other strategic activities. Considering the strategic dimension assumed by patents at the planning phase, this paper aims to use the patent information available on products and TRIZ tools as an analytical method of product development strategies. An example focusing aerogenerators is presented in order to evaluate the proposed perspective.


Victorio C.P.,Centro Universitario Estadual da Zona Oeste | Victorio C.P.,Federal University of Rio de Janeiro | Leal-Costa M.V.,Federal University of Rio de Janeiro | Schwartz Tavares E.,Federal University of Rio de Janeiro | And 2 more authors.
Photochemistry and Photobiology | Year: 2011

Phyllanthus tenellus is widely used for its antiviral, analgesic and hepatoprotective properties. Although the production of several chemical classes of secondary metabolites is influenced by UV radiation, particularly phenolic compounds, we also know that UV radiation can result in anatomical and developmental damage. However, the morphological, anatomical and phytochemical changes in response to UV-A exposure are generally understudied in the Phyllanthaceae. Therefore, we evaluated the effects of UV-A radiation on plant development and leaf anatomy, as well as the production of secondary metabolites and the contents of carotenoids and chlorophylls a and b, in P. tenellus. To accomplish this, in vitro cultures of P. tenellus were maintained for 60 days under white light (WL) and WL plus UV-A radiation. Results showed different phenotypic responses under additional UV-A, such as high phenolic metabolite production, increasing dimensions of abaxial epidermis and thickness of palisade parenchyma. Compared to plants cultured under WL, UV-A radiation caused damage to plant morphogenesis, including a reduced number of branches and shoots, consequently reducing the rate of proliferation. On the other hand, geraniin, ellagic acid and carotenoid contents increased after UV-A exposure, indicating that this light source is an important resource for inducing phenolic compounds. © 2011 The Authors. Photochemistry and Photobiology © 2011 The American Society of Photobiology.


Victorio C.P.,Centro Universitario Estadual da Zona Oeste | Lage C.L.S.,Instituto Nacional da Propriedade Industrial INPI | Sato A.,Rio de Janeiro State Federal University
Revista Ciencia Agronomica | Year: 2012

The high demand for plant material from Calendula officinalis in the production of herbal medicines and cosmetics, turns the technique of plant-tissue culture into one of the alternatives for the improvement of crops over a short period of time. A protocol for tissue culture was developed from segments of seedlings of C. officinalis, in order to improve the proliferation of shoots and roots. We used a Murashige and Skoog (MS1/2N) medium, reduced to half the concentrations of NH4NO3 and KNO3 to verify the effect of different types of explants (basal, intermediate, and apical), a medium containing beach sand as support instead of agar, and the effect of auxins and cytokinins (TDZ tidiazuron; BAP, 6-benzylaminopurine, IAA, indol-3-acetic acid, IBA, indol-3-butyric acid, NAA, naphthalene-acetic acid) on plant development in vitro. The results showed pronounced rooting from the apical explants, as well as a greater elongation of shoots and number of leaves. The solid medium was more suitable for the C. officinalis cultures. Shoot proliferation was dependent on cytokinins with better results from the influence of TDZ or BAP compared to the other treatments. Plants regenerated from the medium containing TDZ displayed a glazed appearance and morphogenetic deformations. The highest rate for rooting (80%) was obtained using IAA 0.1mgL -1. Through in-vitro propagation, healthy C. officinalis plants were obtained with roots which can acclimatise, allowing the continuous supplement of raw material.


De Souza E.R.,Pontifical Catholic University of Rio de Janeiro | De Souza E.R.,Instituto Nacional da Propriedade Industrial INPI | Mont'Alvao C.,Pontifical Catholic University of Rio de Janeiro
Work | Year: 2012

It is essential that access to information must to be universal given the importance of having a truly global Internet. This study proposes to evaluate a government website with two semiautomatic accessibility evaluation tools. The results have demonstrated that the use of more than one semi-automatic assessment tool can provide enhanced results. The differences in results and presentation in the reports generated by the two tools indicate that there is more complete results by combining tests with different accessibility evaluation tools. © 2012 - IOS Press and the authors. All rights reserved.


Cavalheiro G.M.D.C.,Getulio Vargas Foundation | Cavalheiro G.M.D.C.,Instituto Nacional da Propriedade Industrial INPI | Joia L.A.,Getulio Vargas Foundation | Goncalves A.C.,FINEP
World Patent Information | Year: 2014

For most of its history, Brazil needed to import oil to complement its internal production to supply the internal demand. However, in 2007, the Brazilian Federal Government announced the discovery of huge hydrocarbon resources in the pre-salt layer of the country's Southeastern coast. This study examines the impact of this discovery accomplished by Petróleo Brasileiro S.A. (Petrobras) on patent applications in Brazil associated with upstream oil and gas technologies. Then, this article provides empirical evidence that the pre-salt discovery significantly affected patent strategizing of Multi-Nationals Companies (MNCs) operating in the upstream oil and gas industry, thereby generating a boom in patent filings in Brazil from the official pre-salt announcement onwards. © 2014 Elsevier Ltd.


Barreto C.V.G.,Instituto Nacional da Propriedade Industrial INPI | Ferrarezi R.S.,Instituto Nacional da Propriedade Industrial INPI | Ferrarezi R.S.,University of Georgia | Arruda F.B.,Agronomic Institute IAC | Testezlaf R.,University of Campinas
HortScience | Year: 2015

Citrus rootstock production in Brazil commonly uses manual overhead irrigation systems to water plants. Manual irrigation systems present low efficiency, apply more water than needed, and result in release of nutrients and pesticides into the soil with a potential to contaminate groundwater. Closed irrigation systems that avoid the disposal of nutrient solutions like subirrigation can be used to increase production efficiency and reduce the environmental contamination. Our objective was to evaluate the effect of subirrigation applied by a prototype tray on plant growth and morphological and physiological responses of Rangpur lime (Citrus limonia Osbeck ‘Limeira’) seedlings subjected to different water levels in conic containers filled with pine bark substrate. We tested three treatments: T1) subirrigation with water reaching two-thirds of the container height (8 cm); T2) subirrigation with water reaching one-third of the container height (4 cm); and T3) control with manual overhead irrigation. Subirrigation resulted in higher plant growth of Rangpur lime seedlings. At 90 days after sowing (DAS), we observed significant effects of T1 over the other treatments on plant growth, as indicated by higher total dry mass (P = 0.0057), shoot/root ratio (P = 0.0089), shoot height (P = 0.0004), leaf area (P = 0.0005), and root length (P = 0.0333). The number of bifurcations was 400%higher in T3 than at the subirrigated treatments, which can lead to an increase in the labor costs for pruning. Seedlings grown under T1 presented leaf water potential 13%higher compared with T3 at predawn, which was the time of highest stomatal efficiency, presenting the lowest water loss, maximum stomatal closure, and higher transpiration at lower stomatal resistance. T2 plants displayed intermediate water status with a water potential 5% higher than T3. T3 plants showed a higher transpiration rate under maximum stomatal closure, reducing leaf water potential. The subirrigated treatment with water level of two-thirds of container height (8 cm) induced higher plant growth and shortened the crop cycle, anticipating the transplanting to the next phase (grafting) with the possibility of reducing production costs in the nursery. © 2015, American Society for Horticultural Science. All rights reserved.

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