Perez-Jimenez M.,Instituto Murciano Of Investigacion And Desarrollo Agrario And Alimentario Imida |
Cantero-Navarro E.,CSIC - Center of Edafology and Applied Biology of the Segura |
Perez-Alfocea F.,CSIC - Center of Edafology and Applied Biology of the Segura |
Cos-Terrer J.,Instituto Murciano Of Investigacion And Desarrollo Agrario And Alimentario Imida
Plant Physiology and Biochemistry | Year: 2014
Organogenesis in peach (Prunus persica L. Batsch) and peach rootstocks (P. persica×. Prunus dulcis) has been achieved and the action of the regeneration medium on 7 phytohormones, zeatin (Z), zeatin riboside (ZR), indole-3-acetic acid (IAA), abscisic acid (ABA), ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), salicylic acid (SA), and jasmonic acid (JA), has been studied using High performance liquid chromatography - mass spectrometry (HPLC-MS/MS). Three scion peach cultivars, 'UFO-3', 'Flariba' and 'Alice Bigi', and the peach×almond rootstocks 'Garnem' and 'GF677' were cultured in two different media, Murashige and Skoog supplemented with plant growth regulators (PGRs) (regeneration medium) and without PGRs (control medium), in order to study the effects of the media and/or genotypes in the endogenous hormones content and their role in organogenesis. The highest regeneration rate was obtained with the peach×almond rootstocks and showed a lower content of Z, IAA, ABA, ACC and JA. Only Z, ZR and IAA were affected by the action of the culture media. This study shows which hormones are external PGRs-dependent and what is the weight of the genotype and hormones in peach organogenesis that provide an avenue to manipulate invitro organogenesis in peach. © 2014 Elsevier Masson SAS.