Instituto Multidisciplinario Of Biologia Vegetal Uncconicet

Spain

Instituto Multidisciplinario Of Biologia Vegetal Uncconicet

Spain
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Morero R.,Instituto Multidisciplinario Of Biologia Vegetal Uncconicet | Morero R.,National University of Cordoba | Giorgis M.A.,Instituto Multidisciplinario Of Biologia Vegetal Uncconicet | Condack J.P.S.,Federal University of Rio de Janeiro | And 3 more authors.
Boletin de la Sociedad Argentina de Botanica | Year: 2015

In this paper, two new records for the Argentina fern-flora are reported: P. subintegerrimum (Hook. & Arn.) R. Rodr. found in the Patagonian Forests and P. platylepis Fée collected in Misiones; both have been considered endemic for Chile and Brazil, respectively. The species are described and illustrated.


Blanco S.,Instituto Multidisciplinario Of Biologia Vegetal Uncconicet | Penas M.L.L.,Instituto Multidisciplinario Of Biologia Vegetal Uncconicet | Bernardello G.,Instituto Multidisciplinario Of Biologia Vegetal Uncconicet | Stiefkens L.,Instituto Multidisciplinario Of Biologia Vegetal Uncconicet
Boletin de la Sociedad Argentina de Botanica | Year: 2012

The clade Lycieae (Solanaceae) embraces 92 species, currently gathered in a single genus, Lycium. A study was conducted in six South American species of this genus, using the FISH technique for the first time in the group, in addition to the CMA/DAPI banding technique. Root tips of the following species were employed: L. boerhaviifolium (previously Grabowskia), L. bridgesii (previously Phrodus), the tetraploid L. chilense and the diploids L. cestroides, L. ciliatum and L. tenuispinosum. The basic number x=12 was confirmed. The banding technique revealed CMA+/DAPI- bands associated with NORs in the first m pair in the diploid species and in the first two pairs of the tetraploid. In addition, L. tenuispinosum showed an intercalary CMA+/DAPI- band, while in L. bridgesii terminal and intercalary bands were found in all chromosomes. The FISH technique showed that the 18-5.8-26S loci were consistent with CMA+/DAPI-/ NORs blocks. The diploid species had always one m pair carrying 5S rDNA genes, while the tetraploid presented two pairs, consistently with its ploidy level. The diversification of the studied species was not accompanied by significant structural chromosomal rearrangements, except for L. bridgesii which is outstanding because it has a different karyotype formula and a higher heterochromatin percentage.


Chiapero A.L.,Instituto Multidisciplinario Of Biologia Vegetal Uncconicet | Penas M.L.L.,Instituto Multidisciplinario Of Biologia Vegetal Uncconicet | Garcia M.T.A.,CONICET | Bernardello G.,Instituto Multidisciplinario Of Biologia Vegetal Uncconicet
Boletin de la Sociedad Argentina de Botanica | Year: 2013

Passifora has nearly 525 spp. with x = 9 or 6. A cytogenetic study of four species from subgenus Passifora that grow in Argentina was performed. Giemsa technique, fuorescent chromosome banding CMA/DAPI and FISH (probe 18-5,8-26S and 5S) were used in mitotic chromosomes. P. caerulea, P. tucumanensis and P. cincinnata resulted diploid (2n = 18) while P. mooreana tetraploid (2n = 36). The karyotypes were highly symmetrical, with the formula 8 m + 1 sm- , duplicated in P. mooreana. With CMA/DAPI, diploids showed 2-3 pairs of terminal bands CMA /DAPI , whereas the tetraploid fve bands, associated t+o NOR-in all cases. With FISH, the loci 18-5.8-26S coincided in number and position with the CMA /DAPI bands. In each species, the number of sites for 5S rDNA genes agreed with the ploidy level. Data of P. tucumanensis and P. mooreana are here reported for the frst time, being this last species the frst known tetraploid for subgenus Passifora. FISH patterns and heterochromatic bands varied among the Passifora species studied, mainly with respect to the number and distribution of the signals.

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