Belo Horizonte, Brazil
Belo Horizonte, Brazil

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Iasbik A.F.,Instituto Mineiro Of Agropecuaria | Pinto P.S.A.,Federal University of Viçosa | Bevilacqua P.D.,Federal University of Viçosa | Nero L.A.,Federal University of Viçosa | And 2 more authors.
Ciencia Rural | Year: 2010

Taeniasis occurs due to the presence of the adult form of Taenia sp. in human beings, and cysticercosis is caused by the presence of the larval stage of T. solium in pigs and humans. A transversal study was conducted aiming to determine the prevalence of porcine cysticercosis and taeniasis in 176 farms located in rural areas of Viçosa-MG. Blood samples of 226 pigs, and feces samples of 266 humans were collected. An epidemiological survey has also been done. We found a prevalence of 0.4% of porcine cysticercosis and taeniasis has not been found. The epidemiological data has shown that although most people consumed non-inspected pork, it used to be prepared well-done. Data showed that 88.1% of the pigs are reared in confinement in only 1.1% of the properties the sewer was disposed on the soil, and 99.4% used pipes for water distribution. It can be concluded that the prevalence of the taeniasis-cysticercosis complex in rural areas of Viçosa (State of Minas Gerais) is lower than in other similar regions.


Castro M.M.,Federal University of Viçosa | Kim B.,Harvard University | Hill E.,Harvard University | Fialho M.C.Q.,Federal University of Amazonas | And 4 more authors.
Histochemistry and Cell Biology | Year: 2016

Desmodus rotundus is a vampire bat species that inhabits Latin America. Some basic aspects of this species’ biology are still unknown, as the histophysiological characteristics of the male reproductive tract. Our study has focused on its epididymis, which is an important organ for performing a variety of functions, especially the sperm maturation and storage. The aim of this study was to identify principal, narrow, clear, and basal cells using cell-specific markers such as aquaporin 9 (AQP9), vacuolar H+-ATPase (V-ATPase), and cytokeratin 5 (KRT5). Principal cells were labeled by AQP9 from initial segment to cauda region in their stereocilia. They were shown with a columnar shape, whereas V-ATPase-rich cells were identified with a goblet-shaped body along the entire epididymis, including the initial segment, which were named as clear cells. Pencil-shaped V-ATPase-rich cells (narrow cells) were not detected in the initial segment of the bat epididymis, unlike in the rodent. Basal cells were labeled by KRT5 and were located at the basal portion of the epithelium forming a dense network. However, no basal cells with a luminal-reaching body extension were observed in the bat epididymis. In summary, epithelial cells were identified by their specific markers in the vampire bat epididymis. Principal and basal cells were labeled by AQP9 and KRT5, respectively. Narrow cells were not observed in the vampire bat epididymis, whereas clear cells were identified by V-ATPase labeling along the entire duct in a goblet-shaped body. In addition, no luminal-reaching basal cells were observed in the vampire bat epididymis. © 2016 Springer-Verlag Berlin Heidelberg


Braga A.R.C.,Instituto Mineiro Of Agropecuaria | Correa A.P.F.L.,São Paulo State University | Camossi L.G.,São Paulo State University | Da Silva R.C.,São Paulo State University | And 2 more authors.
Revista da Sociedade Brasileira de Medicina Tropical | Year: 2014

Introduction: Leishmaniasis and toxoplasmosis are important to public health. Methods: Antibodies for Toxoplasma gondii and Leishmania spp. were evaluated in cats from Campo Grande, State of Mato Grosso do Sul, Brazil, a region endemic for canine visceral leishmaniasis. Serum samples from 50 asymptomatic cats were titrated for T. gondii by the immunofluorescence antibody test and modified agglutination test and for Leishmania spp. by the immunofl uorescence antibody test. Results: These two agents coinfected two (4%) of the 50 tested animals. Conclusions: These findings demonstrate the concomitant presence of two important zoonoses in cats from Brazilian endemic regions for canine visceral leishmaniasis. © 2014, Sociedade Brasileira de Medicina Tropical. All Rights Reserved.


Rosa R.,Federal University of Minas Gerais | Costa E.A.,Federal University of Minas Gerais | Marques R.E.,Federal University of Minas Gerais | Oliveira T.S.,Federal University of Minas Gerais | And 6 more authors.
PLoS Neglected Tropical Diseases | Year: 2013

St. Louis encephalitis virus (SLEV) is a causative agent of encephalitis in humans in the Western hemisphere. SLEV is a positive-sense RNA virus that belongs to the Flavivirus genus, which includes West Nile encephalitis virus, Japanese encephalitis virus, Dengue virus and other medically important viruses. Recently, we isolated a SLEV strain from the brain of a horse with neurological signs in the countryside of Minas Gerais, Brazil. The SLEV isolation was confirmed by reverse-transcription RT-PCR and sequencing of the E protein gene. Virus identity was also confirmed by indirect immunofluorescence using commercial antibodies against SLEV. To characterize this newly isolated strain in vivo, serial passages in newborn mice were performed and led to hemorrhagic manifestations associated with recruitment of inflammatory cells into the central nervous system of newborns. In summary this is the first isolation of SLEV from a horse with neurological signs in Brazil. © 2013 Rosa et al.


PubMed | University of Sao Paulo, Federal University of Minas Gerais, University of Brasilia and Instituto Mineiro Of Agropecuaria
Type: Journal Article | Journal: Preventive veterinary medicine | Year: 2015

The present survey aimed at estimating the seroprevalence of brucellosis in working equines of cattle farms from Minas Gerais State, Brazil, and investigating risk factors associated with the infection. Serum samples from 6439 animals, including 5292 horses, 1037 mules and 110 donkeys, were collected from 1936 herds, between September 2003 and March 2004, in 848 municipalities from the state of Minas Gerais, Brazil. The prevalence of antibodies against smooth Brucella spp. found in equines from Minas Gerais State was 1.37% (95% CI: 0.97-1.78), resulting in a prevalence of herds with infected animals of 4.28% (95% CI: 4.21-4.36). There were differences between regions but these were not of major epidemiological relevance nor were most of them statistically significant, given the considerable overlap of confidence intervals. Nevertheless, the point estimates suggest that the three northeastern regions have slightly higher prevalence than the rest of the state, both at the herd and animal levels. No association of Brucella spp. seropositivity with sex, age or host was observed. In conclusion, the present study showed a low but widespread prevalence of antibodies against smooth Brucella in equines kept in cattle farms in Minas Gerais, a state where bovine brucellosis is also widespread albeit with low prevalence.


Figueiredo T.C.,Federal University of Minas Gerais | Assis D.C.,Federal University of Minas Gerais | Menezes L.D.,Instituto Mineiro Of Agropecuaria | Oliveira D.D.,Aviario Santo Antonio | And 4 more authors.
Poultry science | Year: 2014

This study was carried out with the aim of evaluating the effects of mineral oil application on eggshells and the use of plastic packages with lids on the physical-chemical and microbiological quality and biogenic amine contents of eggs stored under refrigeration for up to 125 d. A total of 1,920 eggs from 46-wk-old Hyline W36 laying hens were randomly distributed into 4 groups soon after classification: (i) 480 eggs were stored in pulp carton tray packages; (ii) 480 eggs were stored in plastic packages with lids; (iii) 480 eggs were stored in carton packages after the application of mineral oil; and (iv) 480 eggs were stored in plastic packages with lids after the application of mineral oil. The internal quality was measured by Haugh units, by the counts of mesophilic and psychrotrophic microorganisms, by the most probable number of total and thermal-tolerant coliforms, by the counts of molds and yeasts, by the analysis of Salmonella spp. and Staphylococcus spp., and by the levels of biogenic amines in the egg yolk and albumen. The application of mineral oil to the eggshell resulted in higher Haugh unit values throughout storage, and the use of plastic packages altered the internal quality. The application of mineral oil and the use of packaging had no effects on the microbiological and biogenic amine results. Microbiological analyses showed the absence of Salmonella spp., Staphylococcus aureus, thermal-tolerant coliforms, and fungi. However, the highest counts of mesophilic (1.1 × 10(7) cfu/g) and psychrotrophic (6.7 × 10(7) cfu/g) microorganisms were recorded. The highest values of biogenic amines detected and quantified were putrescine (2.38 mg/kg) and cadaverine (7.27 mg/kg) in the egg yolk and putrescine (1.95 mg/kg), cadaverine (2.83 mg/kg), and phenylethylamine (2.57 mg/kg) in the albumen. Despite these results, the biogenic amine levels recorded were considered low and would not be harmful to consumer health. ©2014 Poultry Science Association Inc.


Da Silva J.B.,São Paulo State University | Rangel C.P.,Instituto Mineiro Of Agropecuaria | De Azevedo Baeta B.,Federal Rural University of Rio de Janeiro | Da Fonseca A.H.,Federal Rural University of Rio de Janeiro
Ticks and Tick-borne Diseases | Year: 2013

The purpose of this study was to identify the influence of physiological state, season, breed, number of lactations, dairy productivity, and stocking rate on infestation by Rhipicephalus microplus in dairy cows. Two hundred cows were selected through proportional representative sampling at the Seropédica Experimental Station (Pesagro-Rio), Brazil. Fully or partially engorged R. microplus females measuring between 4.5 and 8.0. mm were counted on the right side of each animal. The tick infestation prevalence data were analyzed in relation to the risk factors, using multiple logistic regression. Associations between prevalence and its possible influencing determinants were measured using odds ratios. The average tick count of cows during the peripartum and lactation periods was significantly higher (p<0.05; OR=4.82) than the count in dry cows. Taurine animals showed significantly higher infestation (p<0.05; OR=3.28) than pure zebuine animals. Among cross-bred animals, the infestation was higher (p<0.05) in F1 animals (1/2 taurine × 1/2 zebuine) than in Girolando animals (5/8 zebuine × 3/8 taurine). Primiparous cows (p<0.05) had significantly heavier infestations than multiparous cows. Dairy production showed a positive correlation with tick infestation (p<0.03; OR=2.94), such that the most productive animals were the most parasitized ones. Animals kept at high densities were significantly more heavily infested (p<0.05, OR=6.32) than animals kept at low density. First-lactation and high-productivity taurine animals were more vulnerable to R. microplus, thus comprising a high-risk group in dairy herds. © 2012 Elsevier GmbH.


PubMed | Fundacao Ezequiel Dias, Aviario Santo Antonio, Federal University of Minas Gerais and Instituto Mineiro Of Agropecuaria
Type: Journal Article | Journal: Poultry science | Year: 2014

This study was carried out with the aim of evaluating the effects of mineral oil application on eggshells and the use of plastic packages with lids on the physical-chemical and microbiological quality and biogenic amine contents of eggs stored under refrigeration for up to 125 d. A total of 1,920 eggs from 46-wk-old Hyline W36 laying hens were randomly distributed into 4 groups soon after classification: (i) 480 eggs were stored in pulp carton tray packages; (ii) 480 eggs were stored in plastic packages with lids; (iii) 480 eggs were stored in carton packages after the application of mineral oil; and (iv) 480 eggs were stored in plastic packages with lids after the application of mineral oil. The internal quality was measured by Haugh units, by the counts of mesophilic and psychrotrophic microorganisms, by the most probable number of total and thermal-tolerant coliforms, by the counts of molds and yeasts, by the analysis of Salmonella spp. and Staphylococcus spp., and by the levels of biogenic amines in the egg yolk and albumen. The application of mineral oil to the eggshell resulted in higher Haugh unit values throughout storage, and the use of plastic packages altered the internal quality. The application of mineral oil and the use of packaging had no effects on the microbiological and biogenic amine results. Microbiological analyses showed the absence of Salmonella spp., Staphylococcus aureus, thermal-tolerant coliforms, and fungi. However, the highest counts of mesophilic (1.1 10(7) cfu/g) and psychrotrophic (6.7 10(7) cfu/g) microorganisms were recorded. The highest values of biogenic amines detected and quantified were putrescine (2.38 mg/kg) and cadaverine (7.27 mg/kg) in the egg yolk and putrescine (1.95 mg/kg), cadaverine (2.83 mg/kg), and phenylethylamine (2.57 mg/kg) in the albumen. Despite these results, the biogenic amine levels recorded were considered low and would not be harmful to consumer health.


This work was carried out based on the hypothesis that polymers associated with silicone (Si) would be obstructing tracheid, therefore contributing to a nutritional collapse of Pinus caribaea forest bulks, in the Triângulo Mineiro and Alto Paranaíba (MG) plains. Previous studies showed a narrow relationship between these physiologic patterns with nutritional deficiencies. However, it is known that seeds used in the establishment of these bulks presented great genetic variability well expressed in diversity of plant behavior, inasmuch as part of them remains healthy and the others start a changeable intensity senescence, which ends up in the death of the plant. In this study, it was sampled segments of root and apical shoots of apparently normal (green needles) plants at different senescence stages (yellow needles) and dead plants. Those samples, after being dried until critical point, were observed by scanning electron microscope coupled to an X-ray microprobe. It was noted the presence of Si and Al in xylem and phloem tissues in the aerial section as well as in the roots, and the greatest contents were found in xylem of the plants. However, it was not observed any obstruction or any presence of Si-Al bodies in the conducting tissues of the analyzed parts. But, it was observed that the presence of Si associated to Al occurred according to a 2.6 order Al/Si ratio. Data also revealed that Si variation amplitude is 1.5 times greater than the Al variation amplitude. It concluded that Si variation amplitude is greater than the one of Al, which establishes a variation relationship (Si-Al).


Silva G.C.,Federal University of Goais | Nascimento M.R.B.M.,Federal University of Uberlandia | Penha-Silva N.,Federal University of Uberlandia | Fernandes E.A.,Federal University of Uberlandia | And 2 more authors.
Revista Ceres | Year: 2015

Broilers raised under heat stress can have reduced antioxidant standard, however supplementation with zinc and selenium can mitigate this effect.In this study, we evaluated the effects of zinc and selenium supplementation, in the organic and inorganic forms, on performance, lipid peroxidation and carcass yield of broilers subjected to cyclic heat stress (HS). A total of 2400 one-day-old male chicks were raised in thermal comfort until the 14th day, then 1200 chicks were subjected to HS. The experiment was arranged in a completely randomized design in a 2 x 5 factorial with 2 environmental conditions [comfort (C) and HS] and 5 supplementation levels [S1) control diet; S2) S1 + 40 mg / kg of inorganic Zn; S3) S1 + 40 mg/kg of organic Zn; S4) S1 + 40 mg / kg of inorganic Zn and 0.2 mg / kg organic selenium and S5) S1 + 40 mg / kg of organic Zn and 0.2 mg / kg selenium organic], totalling 10 treatments with 8 replicates of 30 birds each. The variables evaluated were blood levels of thiobarbituric acid reactive substances (TBARS), cloacal temperature (CT), weight gain, feed intake, feed conversion, live weight and carcass yield and cuts. On the 35th and 42nd days, HS broilers showed increased cloacal temperature. Weight gain, feed conversion and eviscerated carcass were negatively influenced by heat. Environment and supplement levels did not affect breast meat yield with and without bone, drumstick, thigh, and wing. In the environmental and nutritional conditions of this study, there was no need for supplemental addition of organic and inorganic zinc and organic selenium.

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