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São Caetano do Sul, Brazil

Souza L.P.,Instituto Maua Of Tecnologia Imt | Lullio T.G.,Instituto Maua Of Tecnologia Imt | Ratusznei S.M.,Instituto Maua Of Tecnologia Imt | Rodrigues J.A.D.,Instituto Maua Of Tecnologia Imt | Zaiat M.,University of Sao Paulo
Applied Biochemistry and Biotechnology | Year: 2015

An anaerobic sequencing batch reactor with immobilized biomass (AnSBBR) was applied to the production of biohydrogen treating a glucose-based wastewater. The influence of the applied volumetric organic load was studied by varying the concentration of influent at 3600 and 5250 mg chemical oxygen demand (COD) L−1 and cycle lengths of 4, 3, and 2 h resulting in volumetric organic loads of 10.5 to 31.1 g COD L−1. The results revealed system stability in the production of biohydrogen and substrate consumption. The best performance was an organic removal (COD) of 24 % and carbohydrate removal (glucose) of 99 %. Volumetric and specific molar productivity were 60.9 mol H2 m−3 day−1 and 5.8 mol H2 kg SVT−1 day−1 (biogas containing 40 % H2 and no CH4) at 20.0 g COD L−1 day−1 (5250 mg COD L−1 and 3 h). The yield between produced hydrogen and removed organic matter in terms of carbohydrates was 0.94 mol H2 Mol GLU−1 (biogas containing 52 % H2 and no CH4) at 10.5 g COD L−1 day−1 (3600 mg COD L−1 and 4 h), corresponding to 23 and 47 % of the theoretical values of the acetic and butyric acid metabolic routes, respectively. Metabolites present at significant amounts were ethanol, acetic acid, and butyric acid. The conditions with higher influent concentration and intermediate cycle length, and the condition with lower influent concentration and longer cycle showed the best results in terms of productivity and yield, respectively. This indicates that the best productivity tends to occur at higher organic loads, as this parameter involves the biogas production, and the best yield tends to occur at lower and/or intermediate organic loads, as this parameter also involves substrate consumption. © 2015, Springer Science+Business Media New York. Source


Lovato G.,Instituto Maua Of Tecnologia Imt | Bezerra R.A.,Instituto Maua Of Tecnologia Imt | Rodrigues J.A.D.,Instituto Maua Of Tecnologia Imt | Ratusznei S.M.,Instituto Maua Of Tecnologia Imt | Zaiat M.,University of Sao Paulo
Applied Biochemistry and Biotechnology | Year: 2012

The aim of this work was to investigate the effect of different feeding times (2, 4 and 6 h) and applied volumetric organic loads (4.5, 6.0 and 7.5 gCOD L -1 day -1) on the performance of an anaerobic sequencing batch biofilm reactor (AnSBBR) treating effluent from biodiesel production. Polyurethane foam cubes were used as inert support in the reactor, and mixing was accomplished by recirculating the liquid phase. The effect of feeding time on reactor performance showed to be more pronounced at higher values of applied volumetric organic loads (AVOLs). Highest organic material removal efficiencies achieved at AVOL of 4.5 gCOD L -1 day -1 were 87 % at 4-h feeding against 84 % at 2-h and 6-h feeding. At AVOL of 6.0 gCOD L -1 day -1, highest organic material removal efficiencies achieved with 4-h and 6-h feeding were 84 %, against 71 % at 2-h feeding. At AVOL of 7.5 gCOD L -1 day -1, organicmaterial removal efficiency achieved with 4-h feeding was 77%. Hence, longer feeding times favored minimization of total volatile acids concentration during the cycle as well as in the effluent, guaranteeing process stability and safety. © Springer Science+Business Media, LLC 2012. Source


Lima D.M.F.,University of Sao Paulo | Lazaro C.Z.,University of Sao Paulo | Rodrigues J.A.D.,Instituto Maua Of Tecnologia Imt | Ratusznei S.M.,Instituto Maua Of Tecnologia Imt | Zaiat M.,University of Sao Paulo
Journal of Environmental Management | Year: 2016

The present study investigated the influence of the influent concentration of substrate, feeding time and temperature on the production of biohydrogen from cheese whey in an AnSBBR with liquid phase recirculation. The highest hydrogen yield (0.80 molH2.molLactose-1) and productivity (660 mLH2 L-1 d-1) were achieved for influent concentrations of 5400 mgDQO L-1. No significant difference was noted in the biological hydrogen production for the feeding time conditions analyzed. The lowest temperature tested (15 °C) promoted the highest hydrogen yield and productivity (1.12 molH2 molLactose-1 and 1080 mLH2 L-1 d-1), and for the highest temperature (45 °C), hydrogen production did not occur. The indicator values for the hydrogen production obtained with this configuration were higher than those obtained in other studies using traditional configurations such as UASBr and CSTR. A phylogenetic analysis showed that the majority of the analyzed clones were similar to Clostridium. In addition, clones phylogenetically similar to the Lactobacilaceae family, notably Lactobacillus rhamnosus, and clones with similar sequences to Acetobacter indonesiensis were observed in small proportion in the reactor. © 2015 Elsevier Ltd. Source


Inoue R.K.,University of Sao Paulo | Lima D.M.F.,University of Sao Paulo | Rodrigues J.A.D.,Instituto Maua Of Tecnologia Imt | Ratusznei S.M.,Instituto Maua Of Tecnologia Imt | Zaiat M.,University of Sao Paulo
Applied Biochemistry and Biotechnology | Year: 2014

This study investigated the feasibility to produce biohydrogen of a mechanically stirred anaerobic sequencing batch biofilm reactor (AnSBBR) treating sucrose-based synthetic wastewater. The bioreactor performance (30 °C) was evaluated as to the combined effect of fill time (2, 1.5, and 1 h), cycle length (4, 3, and 2 h), influent concentration (3,500 and 5,250 mg chemical oxygen demand (COD) L-1) and applied volumetric organic load (AVOLCT from 9.0 to 27.0 g COD L-1 d-1). AVOLs were varied according to influent concentration and cycle length (tC). The results showed that increasing AVOLCT resulted in a decrease in sucrose removal from 99 to 86 % and in improvement of molar yield per removed load (MYRLS.n) from 1.02 mol H2 mol carbohydrate-1 at AVOLCT of 9.0 g COD L-1 d-1 to maximum value of 1.48 mol H2 mol carbohydrate-1, at AVOLCT of 18.0 g COD L-1 d-1, with subsequent decrease. Increasing AVOLCT improved the daily molar productivity of hydrogen (MPr) from 15.28 to 49.22 mol H2 m-3 d-1. The highest daily specific molar productivity of hydrogen (SMPr) obtained was 8.71 mol H2 kg TVS-1 d-1 at an AVOLCT of 18.0 g COD L-1 d-1. Decreasing tC from 4 to 3 h decreased sucrose removal, increased MPr, and improved SMPr. Increasing influent concentration decreased sucrose removal only at tC of 2 h, improved MYRLS,n and MPr at all tC, and also improved SMPr at tC of 4 and 3 h. Feeding strategy had a significant effect on biohydrogen production; increasing fill time improved sucrose removal, MPr, SMPr, and MYRLS,n for all investigated AVOLCT. At all operational conditions, the main intermediate metabolic was acetic acid followed by ethanol, butyric, and propionic acids. Increasing fill time resulted in a decrease in ethanol concentration. © 2014 Springer Science+Business Media New York. Source


Manssouri M.,University of Sao Paulo | Rodrigues J.A.D.,Instituto Maua Of Tecnologia Imt | Ratusznei S.M.,Instituto Maua Of Tecnologia Imt | Zaiat M.,University of Sao Paulo
Applied Biochemistry and Biotechnology | Year: 2013

An anaerobic sequencing batch biofilm reactor (AnSBBR - total volume 7.5 L; liquid volume 3.6 L; treated volume per cycle 1.5 L) treated sucrose-based wastewater to produce biohydrogen (at 30 C). Different applied volumetric organic loads (AVOL of 9.0, 12.0, 13.5, 18.0, and 27.0 kg COD m-3 day-1), which were varied according to the influent concentration (3,600 and 5,400 mg COD L-1) and cycle length (4, 3, and 2 h), have been used to assess the following parameters: productivity and yield of biohydrogen per applied and removed load, reactor stability, and efficiency. The removed organic matter (COD) remained stable and close to 18 % and carbohydrates (sucrose) uptake rate remained between 83 and 97 % during operation. The decrease in removal performance of the reactor with increasing AVOL, by increasing the influent concentration (at constant cycle length) and decreasing the cycle lengths (at constant influent concentrations), resulted in lower conversion efficiencies. Under all conditions, when organic load increased there was a predominance of acetic, propionic, and butyric acid as well as ethanol. The highest concentration of biohydrogen in the biogas (24-25 %) was achieved at conditions with AVOL of 12.0 and 13.5 kg COD m-3 day -1, the highest daily production rate (0.139 mol H2 day-1) was achieved at AVOL of 18.0 kg COD m-3 day -1, and the highest production yields per removed and applied load were 2.83 and 3.04 mol H2 kg SUC-1, respectively, at AVOL of 13.5 kg COD m-3 day-1. The results indicated that the best productivity tends to occur at higher organic loads, as this parameter involves the "biochemical generation" of biogas, and the best yield tends to occur at lower and/or intermediate organic loads, as this parameter involves "biochemical consumption" of the substrate. © 2013 Springer Science+Business Media New York. Source

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