Sanchez-Hidalgo J.M.,University of Cordoba, Spain |
Naranjo A.,University of Cordoba, Spain |
Ciria R.,University of Cordoba, Spain |
Ciria R.,Imibic Instituto Maimonides Of Investigacion Biomedica Of Cordoba |
And 20 more authors.
Journal of Surgical Research | Year: 2012
Background: Liver resection is a feasible treatment for multiple liver diseases. There is no evidence about the impact of age on liver regeneration. Objective: To assess the effect of age on liver regeneration in an experimental in vivo animal model of 70%-partial hepatectomy. Methods: Forty young (Y) and old (O) Wistar male rats (n = 80) were distributed into four groups [controls (C), sham operated (SO), hepatectomy 6 h (H6), and 48 h (H48)]. Different morphometric and biochemical factors, oxidative and nitrosative stress, lipid peroxidation, cytokines kinetics, and histopathologic tissular parameters were determined. Results: Early postoperative mortality was higher in aged rats (P = 0.049). Morphometric determinations, liver regeneration index, and total volume weight were favorable to young rats. Serum transaminase levels were higher in aged rats. Parameters of necrosis (measured by histopathologic injury [HI: 0-I-II-III]), regeneration (measured by bromodeoxyuridine-BrdU incorporation) and apoptosis (determined by the TDT-mediated dUTP nick end labeling-TUNEL) were well-synchronized in young rats. Parameters of oxidative stress such as reduced (GSH), oxidized (GSSG) glutathione and lipid peroxidation (measured by hepatic malondialdehyde -MDA-) were lower in young animals throughout the studied period. Nitrosative stress measured by nitric oxide (NO) end-products was higher in late stages in resected old rats. Pro-inflammatory cytokines (TNF- α) reached higher and earlier levels in aged rats while pro-regenerative cytokines (IL-6) were significantly higher in early stages for young rats and in late stages for aged rats. The levels of TGF-β were higher in young rats. Conclusion: Liver regeneration is delayed and reduced in aged animals submitted to liver resection. © 2012 Elsevier Inc. All rights reserved.