Instituto Israelita Of Ensino E Pesquisa Albert Einstein Iiepae

São Paulo, Brazil

Instituto Israelita Of Ensino E Pesquisa Albert Einstein Iiepae

São Paulo, Brazil
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Martins-Costa M.C.,Federal University of São Paulo | Martins-Costa M.C.,University of Fortaleza | Maciel R.M.B.,Federal University of São Paulo | Maciel R.M.B.,Instituto Israelita Of Ensino E Pesquisa Albert Einstein Iiepae | And 14 more authors.
Archives of Endocrinology and Metabolism | Year: 2017

Objectives: The presence of thyroglobulin (Tg) in needle washouts of fine needle aspiration biopsy (Tg-FNAB) in neck lymph nodes (LNs) suspected of metastasis has become a cornerstone in the follow-up of patients with papillary thyroid carcinoma (PTC). However, there are limited data regarding the measurement of anti-Tg antibodies in these washouts (TgAb-FNAB), and it is not clear whether these antibodies interfere with the assessment of Tg-FNAB or whether there are other factors that would more consistently justify the finding of low Tg-FNAB in metastatic LNs. Materials and methods: We investigated 232 FNAB samples obtained from suspicious neck LNs of 144 PTC patients. These samples were divided according to the patient’s serum TgAb status: sTgAb- (n = 203 samples) and sTgAb+ (n = 29). The TgAb-FNAB levels were measured using two different assays. Tg-FNAB was also measured using two assays when low levels (< 10 ng/mL) were identified in the first assay of the metastatic LNs from the sTgAb+ samples. Results: The TgAb-FNAB results were negative in both assays in all samples. Low levels of Tg-FNAB were identified in 11/16 of the metastatic LNs of the sTgAb+ patients and 16/63 of the sTgAb- patients (p < 0.05) using assay 1. The measurement of the Tg-FNAB levels using assay 2 indicated additional metastases in 5 LNs of the sTgAb+ patients. Conclusions: Factors other than the presence of TgAb-FNAB may contribute to the higher number of metastatic LNs with undetectable Tg-FNAB in the sTgAb+ group. In addition, the measurement of Tg-FNAB using different assays was useful to enhance the diagnosis of metastatic LNs, particularly when cytological and Tg-FNAB results are discordant. © AE&M all rights reserved.

Sibov T.T.,Hospital Israelita Albert Einstein | Severino P.,Instituto Israelita Of Ensino E Pesquisa Albert Einstein Iiepae | Marti L.C.,Instituto Israelita Of Ensino E Pesquisa Albert Einstein Iiepae | Pavon L.F.,Hospital Israelita Albert Einstein | And 7 more authors.
Cytotechnology | Year: 2012

Isolation of mesenchymal stem cells (MSCs) from umbilical cord blood (UCB) from fullterm deliveries is a laborious, time-consuming process that results in a low yield of cells. In this study we identified parameters that can be helpful for a successful isolation of UCB-MSCs. According to our findings, chances for a well succeeded isolation of these cells are higher when MSCs were isolated from UCB collected from normal full-term pregnancies that did not last over 37 weeks. Besides the duration of pregnancy, blood volume and storage period of the UCB should also be considered for a successful isolation of these cells. Here, we found that the ideal blood volume collected should be above 80 mL and the period of storage should not exceed 6 h. We characterized UCB-MSCs by morphologic, immunophenotypic, protein/gene expression and by adipogenic differentiation potential. Isolated UCB-MSCs showed fibroblast-like morphology and the capacity of differentiating into adipocyte-like cells. Looking for markers of the undifferentiated status of UCB-MSCs, we analyzed the UCB-MSCs' protein expression profile along different time periods of the differentiation process into adipocyte-like cells. Our results showed that there is a decrease in the expression of the markers CD73, CD90, and CD105 that correlates to the degree of differentiation of UCB-MSCs We suggest that CD90 can be used as a mark to follow the differentiation commitment degree of MSCs. Microarray results showed an up-regulation of genes related to the adipogenesis process and to redox metabolism in the adipocyte-like differentiated MSCs. Our study provides information on a group of parameters that may help with successful isolation and consequently with characterization of the differentiated/ undifferentiated status of UCB-MSCs, which will be useful to monitor the differentiation commitment of UCB-MSC and further facilitate the application of those cells in stem-cell therapy. © Springer Science+Business Media B.V. 2012.

Rio A.L.S.,Federal University of São Paulo | Rio A.L.S.,Instituto Israelita Of Ensino E Pesquisa Albert Einstein Iiepae | Biscolla R.P.M.,Federal University of São Paulo | Biscolla R.P.M.,Instituto Israelita Of Ensino E Pesquisa Albert Einstein Iiepae | And 17 more authors.
Arquivos Brasileiros de Endocrinologia e Metabologia | Year: 2011

Objective: To evaluate the risk of malignancy in thyroid nodules through clinical, laboratory, ultrasonographic and cytological aspects. Patients and methods: 741 nodules of 407 patients. Results: The cytology was benign (60,5%), indeterminate (23,3%), malignant (8,3%) or nondiagnostic (7,6%). The prevalence of cancer in indeterminate citology was 18,5% (16% in follicular lesions, 44% in suspicious). The diagnosis of malignancy was 17,2% (n = 70). The frequency of cancer in women (15,2%) was lower than in men (27,9%). There was an inverse relation between age and cancer risk. There was no statistical significance in the prevalence of cancer according to number, size of nodules or TSH levels. Hypoechogenicity and microcalcifications on ultrasound were risk factors. Conclusion: The risk of malignancy was higher in men, hypoechoic nodules, with microcalcifications and was inversely related to age. The TSH level was not an independent factor predictive of malignancy.

Pavon L.F.,Sociedade Beneficente Israelita Brasileira Hospital Albert Einstein SBIBHAE | Marti L.C.,Instituto Israelita Of Ensino E Pesquisa Albert Einstein Iiepae | Sibov T.T.,Sociedade Beneficente Israelita Brasileira Hospital Albert Einstein SBIBHAE | Malheiros S.M.F.,University of Sao Paulo | And 6 more authors.
Cancer Biotherapy and Radiopharmaceuticals | Year: 2010

Despite recent advances, patients with malignant brain tumors still have a poor prognosis. Glioblastoma (WHO grade 4 astrocytoma), the most malignant brain tumor, represents 50% of all astrocytomas, with a median survival rate of <1 year. It is, therefore, extremely important to search for new diagnostic and therapeutic approaches for patients with glioblastoma. This study describes the application of superparamagnetic nanoparticles of iron oxide, as well as monoclonal antibodies, of immunophenotypic significance, conjoined to quantum dots for the ultrastructural assessment of glioblastoma cells. For this proposal, an immunophenotypic study by flow cytometry was carried out, followed by transmission electron microscopy analysis. The process of tumor cell labeling using nanoparticles can successfully contribute to the identification of tumorigenic cells and consequently for better understanding of glioblastoma genesis and recurrence. In addition, this method may help further studies in tumor imaging, diagnosis, and prognostic markers detection. © 2010, Mary Ann Liebert, Inc. 2010.

Borges J.D.,University of Sao Paulo | Borges J.D.,Instituto Israelita Of Ensino E Pesquisa Albert Einstein Iiepae | Souza V.A.U.F.,University of Sao Paulo | Giambartolomei C.,London School of Hygiene and Tropical Medicine | And 8 more authors.
Journal of Infectious Diseases | Year: 2012

Background. The intrafamilial dynamics of endemic infection with human herpesvirus type 8 (HHV-8) in Amerindian populations is unknown.Methods.Serum samples were obtained from 517 Amerindians and tested for HHV-8 anti-latent nuclear antigen (anti-LANA) and antilytic antibodies by immunofluorescence assays. Logistic regression and mixed logistic models were used to estimate the odds of being HHV-8 seropositive among intrafamilial pairs.Results.HHV-8 seroprevalence by either assay was 75.4 (95 confidence interval [CI]: 71.5-79.1), and it was age-dependent (P trend <. 001). Familial dependence in HHV-8 seroprevalence by either assay was found between mother-offspring (odds ratio [OR], 5.44; 95 CI: 1.62-18.28) and siblings aged ≥10 years (OR 4.42, 95 CI: 1.70-11.45) or siblings in close age range (<5 years difference) (OR 3.37, 95 CI: 1.21-9.40), or in families with large (>4) number of siblings (OR, 3.20, 95 CI: 1.33-7.67). In separate analyses by serological assay, there was strong dependence in mother-offspring (OR 8.94, 95 CI: 2.94-27.23) and sibling pairs aged ≥10 years (OR, 11.91, 95 CI: 2.23-63.64) measured by LANA but not lytic antibodies.Conclusions.This pattern of familial dependence suggests that, in this endemic population, HHV-8 transmission mainly occurs from mother to offspring and between close siblings during early childhood, probably via saliva. The mother to offspring dependence was derived chiefly from anti-LANA antibodies. © 2012 The Author.

Nakabashi C.C.D.,Federal University of São Paulo | Nakabashi C.C.D.,Instituto Israelita Of Ensino E Pesquisa Albert Einstein Iiepae | Biscolla R.P.M.,Federal University of São Paulo | Biscolla R.P.M.,Instituto Israelita Of Ensino E Pesquisa Albert Einstein Iiepae | And 9 more authors.
Arquivos Brasileiros de Endocrinologia e Metabologia | Year: 2012

Objective: In the last decade, data published stressed the role of highly-sensitive thyroglobulin (Tg) assays in the follow-up of differentiated thyroid carcinoma (DTC) patients. The present study describes a new, highly-sensitive Tg assay, compares it with an available commercial assay, and validates it in the follow-up of DTC patients. Subjects and methods: The immunofluorometric high-sensitivity Tg assay is based on monoclonal and polyclonal antibodies produced at our laboratories. It was validated in 100 samples of 87 patients with DTC submitted to total thyroidectomy, 87% of whom also received radioiodine. For correlation, all samples were also tested using a commercial Tg assay (Beckman Access) with functional sensitivity (FS) of 0.1 ng/mL. Results: The new method showed FS of 0.3 ng/mL. The correlation between the two methods was good (r = 0.74; p < 0.0001). The diagnostic sensitivity was 88.9%, and it was increased to 100% when combined with neck US. Conclusion: This new, high-sensitivity Tg assay presented a good correlation with Beckman Access assay and with the clinical outcome of the patients. The continuous availability of a validated assay is an additional advantage for long term follow-up of DTC patients. © ABE&M todos os direitos reservados.

Oliveira T.A.,Hospital Leonor Mendes Of Barros | Cunha D.R.D.,Instituto Israelita Of Ensino E Pesquisa Albert Einstein Iiepae | Policastro A.,Hospital Israelita Albert Einstein HIAE | Traina E.,Hospital Israelita Albert Einstein HIAE | And 2 more authors.
Revista Brasileira de Ginecologia e Obstetricia | Year: 2011

Purpose: to investigate the association between gene polymorphism of theprogesterone receptor (PROGINS) and the risk of premature birth. Methods: In this case-control study, 57 women with previous premature delivery (Case Group) and 57 patients with delivery at term in the current pregnancy and no history of preterm delivery (Control Group) were selected. A 10 mL amountof peripheral blood was collected by venipuncture and genomic DNA was extracted followed by the polymerase chain reaction (PCR) under specific conditions for this polymorphism and 2% agarose gel electrophoresis. The bands were visualized with an ultraviolet light transilluminator. Genotype and allele PROGINS frequencies were compared between the two groups by the χ 2 test, with the level of significanceset at value p<0.05. The Odds Ratio (OR) was also used, with 95% confidence intervals. Results: PROGINS genotypic frequencies were 75.4% T1/T1, 22.8% T1/T2 and 1.8% T2/T2 in the Group with Preterm Delivery and 80.7% T1/T1, 19.3% T1/ T2 and 0% T2/T2 in the term Delivery Group. There were no differences between groups when genotype and allele frequencies were analyzed: p=0.4 (OR=0.7) and p=0.4 (OR=0.7). Conclusions: the present study suggests that the presence of PROGINS polymorphism in our population does not constitute a risk factor for premature birth.

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