Instituto Investigacion Biosanitaria

Spain

Instituto Investigacion Biosanitaria

Spain

Time filter

Source Type

Manzano-Moreno F.J.,University of Granada | Manzano-Moreno F.J.,Instituto Investigacion Biosanitaria | Medina-Huertas R.,University of Granada | Ramos-Torrecillas J.,University of Granada | And 5 more authors.
Journal of Cranio-Maxillofacial Surgery | Year: 2015

Objectives The objective of this study was to determine the effect of LLDL therapy on the gene expression of osteoblast markers of growth and differentiation. Materials and methods The MG-63 cell line was exposed to diode laser (ezLase) of 940 nm at 1-1.5 W and 3-4 J, and gene expressions (Runx-2, alkaline phosphatase [ALP], type I collagen [Col-I], osterix [OSX], osteocalcin [OSC], osteoprotegerin [OPG], bone morphogenetic protein [BMP]-2 and -7, transforming growth factor-β1 [TGF-β1], and TGF-β receptors [TGF-β R1, TGF-β R2; TGF-β R3]) were evaluated by quantitative RT-PCR. Results LLDL treatment stimulated the expression of osteoblast differentiation markers ALP, Col-I, Runx-2, and OSX in relation to the doses applied (P < 0.05), but no changes were detected in OSC, OPG, or BMP-7 at any study dose. This effect may be mediated by TGF-β1 and BMP-2, given that the treatment increased their expression and that of TGF-β receptors R1, R2, and R3 (P < 0.001). Conclusion These results suggest that the biostimulatory effect of laser therapy on osteoblasts may be attributable to the release of autocrine factors in response to the irradiation. A clinical trial is warranted to test its therapeutic usefulness in bone tissue regeneration and to define a treatment protocol. © 2015 European Association for Cranio-Maxillo-Facial Surgery.


Garcia-Martinez O.,University of Granada | Garcia-Martinez O.,Instituto Investigacion Biosanitaria | Rivas A.,University of Granada | Ramos-Torrecillas J.,University of Granada | And 5 more authors.
International Journal of Food Sciences and Nutrition | Year: 2014

The incidence of osteoporosis and associated fractures is found to be lower in countries where the Mediterranean diet is predominant. These observations might be mediated by the active constituents of olive oil and especially phenolic compounds. Objective: To review current knowledge by searching for all relevant publications since 2001 in the MEDLINE, EMBASE and Cochrane Library databases, using the descriptors: Mediterranean diet, virgin olive oil, phenols, bone, osteoblast and osteoporosis. Results and conclusions: Published evidence suggests that olive oil phenols can be beneficial by preventing the loss of bone mass. It has been demonstrated that they can modulate the proliferative capacity and cell maturation of osteoblasts by increasing alkaline phosphatase activity and depositing calcium ions in the extracellular matrix. Further research on this issue is warranted, given the prevalence of osteoporosis and the few data available on the action of olive oil on bone. © 2014 Informa UK Ltd. All rights reserved.


Medina-Huertas R.,University of Granada | Medina-Huertas R.,Instituto Investigacion Biosanitaria | Manzano-Moreno F.J.,Instituto Investigacion Biosanitaria | Manzano-Moreno F.J.,University of Granada | And 8 more authors.
Lasers in Medical Science | Year: 2014

Previous in vivo and in vitro studies have reported that low-level diode laser therapy induces a biostimulatory effect, such as cell proliferation. The aim of the present study was to evaluate whether the laser irradiation of osteoblast-like cells (MG-63) can modify alkaline phosphatase activity (ALP), antigenic profile, and phagocytic capacity. The MG-63 cell line was exposed to diode laser (ezLase) of 940 nm at 1-1.5 W/cm2 and 3-4 J. ALP was evaluated by a spectrophotometric technique and antigenic expression analysis (CD 54, CD80, CD86, HLA-DR), and phagocytic activity was analyzed by flow cytometry. At 24 h, the treated groups showed an increased ALP, and the highest increase versus controls (P = 0.002) was at the dose of 1 W/cm2 and 3 J; this modulation of the antigenic profile translated into a reduced expression of CD54, CD86, and HLA-DR and a slightly decreased phagocytic capacity with respect to the nonirradiated control group at the different intensities and fluencies assayed. These results demonstrate that laser therapy can exert a biostimulatory effect on osteoblastic cells at different levels, which may be clinically useful in the regeneration of bone tissue. © 2014 Springer-Verlag.


Ramos-Torrecillas J.,University of Granada | Ramos-Torrecillas J.,Instituto Investigacion Biosanitaria | Garcia-Martinez O.,University of Granada | Garcia-Martinez O.,Instituto Investigacion Biosanitaria | And 5 more authors.
Biological Research for Nursing | Year: 2015

Platelet-rich growth factor (PRGF) is a natural source of growth factors (GF), while hyaluronic acid (HA) is a biopolymer present in the extracellular matrix of skin, cartilage, bone, and brain, among other tissues. Both are involved in the pathophysiological mechanisms underlying wound healing. The objective of this study was to evaluate the clinical efficacy (as measured by ulcer area) and safety (as measured by signs of infection) of PRGF and PRGF plus HA in the treatment of pressure ulcers (PUs). Patients (N = 100) with 124 Stage II–III PUs were randomized to a control group (n = 25 PUs) for standard care or to case groups for treatment with one (n = 34 PUs) or two (n = 25 PUs) doses of PRGF from their own peripheral blood, or two doses of PRGF plus HA (n = 40 PUs). All ulcers were followed up every 3 days for a 36-day period. At 36 days, a significant reduction in ulcer area (p ≤.001) was observed in all treatment groups, with a mean reduction of more than 48.0% versus baseline. The greatest mean reduction (80.4% vs. baseline) was obtained with the PRGF plus HA regimen. Complete wound healing was observed in 32.0% of PUs treated with two doses of PRGF (p ≤.002) and in 37.5% of those treated with two doses of PRGF plus HA (p ≤.004). There were no signs of infection in any PUs during the 36-day follow-up period. The degree of wound healing was inversely correlated with the consumption of drugs such as statins and with the peripheral blood platelet levels of patients at baseline. © The Author(s) 2014.


Manzano-Moreno F.J.,University of Granada | Manzano-Moreno F.J.,Instituto Investigacion Biosanitaria | Ramos-Torrecillas J.,University of Granada | Ramos-Torrecillas J.,Instituto Investigacion Biosanitaria | And 6 more authors.
Journal of Cranio-Maxillofacial Surgery | Year: 2015

Objectives The study objective was to evaluate the effect on osteoblast growth of high concentrations of three nitrogen-containing bisphosphonates (pamidronate, alendronate, and ibandronate) and one non-nitrogen-containing bisphosphonate (clodronate), using the MG-63 cell line as an osteoblast model, in order to determine the role of osteoblasts in bisphosphonate-related osteonecrosis of the jaw (BRONJ). Materials and methods Osteoblasts were incubated in culture medium with different doses of pamidronate, alendronate, ibandronate or clodronate. The proliferative capacity of the osteoblasts was determined by spectrophotometry (MTT-based) at 24 h of culture. Flow cytometry was used to determine the percentage of cells in each cell cycle phase (G0/G1, G2/M, and S) and to discriminate apoptotic cell death from necrotic cell death in the cell cycle at 24 h of treatment. Results All the bisphosphonates assayed produced a significant and dose-dependent reduction in MG-63 proliferation at the high doses assayed (10-4 and 5 × 10-5 M) in comparison with controls (p <0.001). Cell cycle study revealed that all assayed bisphosphonates significantly arrested the cell cycle in phase G0/G1 at doses of 10-4 and 5 × 10-5 M, increasing the percentage of cells in this phase (p <0.05). Apoptosis/necrosis studies showed significant changes compared with control cells, with an increased percentage of cells in apoptosis after treatment with 10-4 or 5 × 10-5 M of pamidronate, alendronate, ibandronate, or clodronate (p <0.05). Conclusions High doses of nitrogen-containing or non-nitrogen-containing bisphosphonates can reduce the proliferation of MG-63 osteoblast-like cells by arresting the cell cycle and inducing apoptosis/necrosis. © 2015 European Association for Cranio-Maxillo-Facial Surgery.

Loading Instituto Investigacion Biosanitaria collaborators
Loading Instituto Investigacion Biosanitaria collaborators