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Dos Santos A.A.,Federal University of ABC | Bianco B.,Federal University of ABC | Bianco B.,Instituto Ideia Fertil Of Saude Reprodutiva | Barbosa C.P.,Federal University of ABC | And 3 more authors.
Reproductive BioMedicine Online | Year: 2014

The aim of this study was to investigate the association between MTHFR gene polymorphisms and IVF outcomes in Brazilian women undergoing assisted reproduction treatment. A prospective study was conducted in the Human Reproduction Department at the ABC University School of Medicine and the Ideia Fertility Institute between December 2010 and April 2012. The patient population was 82 women undergoing assisted reproduction cycles. The MTHFR polymorphisms C677T and A1298C were evaluated and compared with laboratory results and pregnancy rates. The C677T variant was associated with proportions of mature (P = 0.006) and immature (P = 0.003) oocytes whereas the A1298C variant was associated with number of oocytes retrieved (P = 0.044). The polymorphisms, whether alone or in combination, were not associated with normal fertilization, good-quality embryo or clinical pregnancy rates. This study suggests that the number and maturity of oocytes retrieved may be related to the MTHFR polymorphisms C677T and A1298C. It is believed that folate has a crucial function in human reproduction and that folate deficiency can compromise the function of the metabolic pathways it is involved in, leading to an accumulation of homocysteine. The gene MTHFR encodes the 5-MTHFR enzyme, which is involved in folate metabolism, and C677T/A1298C polymorphisms of this gene are related to decreased enzyme activity and consequent changes in homocysteine concentration. Folate deficiency and hyperhomocysteinaemia can also compromise fertility and lead to pregnancy complications by affecting the development of oocytes, preparation of endometrial receptivity, implantation of the embryo and pregnancy. In folliculogenesis, hyperhomocysteinaemia can activate apoptosis, leading to follicular atresia and affecting the maturity of oocytes and the quality of embryos cultured in vitro. This study was performed to investigate the association between MTHFR polymorphisms and IVF outcomes in women undergoing assisted reproduction treatment. © 2014, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.


Christofolini D.M.,Instituto Ideia Fertil Of Saude Reprodutiva | Amaro A.,Collective Health | Mafra F.,Collective Health | Sonnewend A.,Collective Health | And 2 more authors.
Journal of Assisted Reproduction and Genetics | Year: 2014

Purpose: Estrogen metabolizing gene mutations can be associated with defective hormonal signaling leading to disease processes. Endometriosis is an estrogen dependent that can be influenced by defective signaling in the estrogen pathway.Objectives: To evaluate the association of A/G 85952 CYP2C19 and A/G 937 HSD17B1 gene polymorphisms with endometriosis through the investigation of a large Brazilian sample of women with endometriosis and a fertile control group.Methods: Five hundred women with endometriosis and 500 women without endometriosis were tested for CYP2C19 and HSD17B1 polymorphisms, by TaqMan Real Time PCR. The results were statistically analyzed by chi-square, logistic regression and tested for Hardy-Weinberg equilibrium.Results: The comparison of genotype and allelic frequency of CYP2C19 polymorphism (rs11592737) in patients with endometriosis and control group showed a statistically significant difference (p = 0.0203) and for the HSD17B1 polymorphism (rs605059) differences were not significant (p = 0.0687). Comparing the stages I/II and III/IV endometriosis with the control group for the CYP2C19 we observed p = 0.0133 and p = 0.0564, respectively, and for HSD17B1 the values for p = 0.4319 and p = 0.0667.Conclusion: We observed that CYP2C19 polymorphism is associated with endometrisis in Brazilian women and can be considered a potential biomarker of the disease. © 2014, Springer Science+Business Media New York.


Santos M.,Instituto Ideia Fertil Of Saude Reprodutiva | Cordts E.B.,Instituto Ideia Fertil Of Saude Reprodutiva | Bianco B.,Instituto Ideia Fertil Of Saude Reprodutiva | Barbosa C.P.,Instituto Ideia Fertil Of Saude Reprodutiva | Christofolini D.M.,Instituto Ideia Fertil Of Saude Reprodutiva
Jornal Brasileiro de Reproducao Assistida | Year: 2015

Objective: The present study consists of quality comparison among oocytes retrieved from women under 37 years old showing increased levels of FSH (prone to premature ovarian insufficiency) and women at the same age with normal hormone levels. Methods: Oocyte quality was accessed according to Lucinda L. Veeck parameters (1986) and the statistical analyses were carried out using Chisquared, SPSS for Windows 13.0 (SPSS, Inc., Chicago, IL). All pvalues were twotailed, and 95% confidence intervals (CIs) were calculated. A P value <0.05 was considered statistically significant. Results: Eight morphologic changes variables were considered in the study and two of them showed statistically significant differences between cases and controls: granular cytoplasm (P=0.002) and presence of vacuoles (P=0.025), both more frequent among the study group patients. Conclusion: As a conclusion, patients with increased FSH levels presented oocytes with worst quality variables than controls. This can be an indicative of ovarian aging and can impact negatively on oocyte development into viable embryos. © 2015, Sociedade Brasileira de Reproducao Assistida. All rights reserved.


PubMed | Instituto Ideia Fertil Of Saude Reprodutiva
Type: Journal Article | Journal: Journal of assisted reproduction and genetics | Year: 2015

Estrogen metabolizing gene mutations can be associated with defective hormonal signaling leading to disease processes. Endometriosis is an estrogen dependent that can be influenced by defective signaling in the estrogen pathway.To evaluate the association of A/G 85952 CYP2C19 and A/G 937 HSD17B1 gene polymorphisms with endometriosis through the investigation of a large Brazilian sample of women with endometriosis and a fertile control group.Five hundred women with endometriosis and 500 women without endometriosis were tested for CYP2C19 and HSD17B1 polymorphisms, by TaqMan Real Time PCR. The results were statistically analyzed by chi-square, logistic regression and tested for Hardy-Weinberg equilibrium.The comparison of genotype and allelic frequency of CYP2C19 polymorphism (rs11592737) in patients with endometriosis and control group showed a statistically significant difference (p=0.0203) and for the HSD17B1 polymorphism (rs605059) differences were not significant (p=0.0687). Comparing the stages I/II and III/IV endometriosis with the control group for the CYP2C19 we observed p=0.0133 and p=0.0564, respectively, and for HSD17B1 the values for p=0.4319 and p=0.0667.We observed that CYP2C19 polymorphism is associated with endometrisis in Brazilian women and can be considered a potential biomarker of the disease.

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