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Moraes A.M.,University of Campinas | Jorge S.A.C.,Instituto Butantan | Astray R.M.,Instituto Butantan | Suazo C.A.T.,Federal University of São Carlos | And 10 more authors.
Biotechnology Advances | Year: 2012

In the present review we discuss strategies that have been used for heterologous gene expression in Drosophila melanogaster Schneider 2 (S2) cells using plasmid vectors. Since the growth of S2 cells is not dependent on anchorage to solid substrates, these cells can be easily cultured in suspension in large volumes. The factors that most affect the growth and gene expression of S2 cells, namely cell line, cell passage, inoculum concentration, culture medium, temperature, dissolved oxygen concentration, pH, hydrodynamic forces and toxic metabolites, are discussed by comparison with other insect and mammalian cells. Gene expression, cell metabolism, culture medium formulation and parameters involved in cellular respiration are particularly emphasized. The experience of the authors with the successful expression of a biologically functional protein, the rabies virus glycoprotein (RVGP), by recombinant S2 cells is presented in the topics covered. © 2011 Elsevier Inc.


Pinho Ferreira L.,Federal University of Ceará | Sabry R.C.,Instituto Federal Of Educacao Ciencia E Tecnologia | da Silva P.M.,Federal University of Paraiba | Gesteira T.C.V.,Federal University of Ceará | And 5 more authors.
Experimental Parasitology | Year: 2015

This is the first report of Perkinsus sp. (Bivalvia: Veneridae) infecting wild clams of the species Anomalocardia brasiliana in Brazil. The gill lamellae and rectum of 150 specimens of A. brasiliana collected in the Timonha river estuary (Ceará, Northeastern Brazil) in March 2012 were incubated in Ray's fluid thioglycollate medium (RFTM) for detection of Perkinsus sp. In RFTM, the prevalence of Perkinsus sp. was 14.7% (22/150) and the intensity of infection ranged from very light (1-10 cells across the slide) to light (12-100 cells). The presence of Perkinsus sp. was confirmed by PCR in seven (31.8%) out of 22 RFTM-positive specimens. DNA sequencing confirmed the presence of the genus Perkinsus and the phylogenetic analysis strongly indicated Perkinsus beihaiensis as the species responsible for the infection. © 2014 Elsevier Inc.


Kormann da Silva M.,Instituto Federal Of Educacao Ciencia E Tecnologia | Goulart Rocha F.,Instituto Federal Of Educacao Ciencia E Tecnologia | Amaral F.M.,Instituto Federal Of Educacao Ciencia E Tecnologia
Journal of Culinary Science and Technology | Year: 2015

Santa Catarina is one of the top Brazilian states regarding fish capture, with the extraction of 139 species. Especially by the coast, the fish stands out as one of the emblems of this state’s culinary dishes, being a representative ingredient on the restaurant’s menu. Purpose: To identify the fish varieties and cooking methods utilized by the restaurants and to assess how the use of local raw material affirms the regional gastronomic identity. Methodology: Administering of questionnaires to restaurants in 21 cities on the coast of Santa Catarina. Findings: Seven fish species were identified as having higher occurrence in the menus of the restaurants. The coast cuisine seems to reveal itself insufficiently characterized. © Taylor & Francis Group, LLC.


Pereira G.S.,Federal University of Ceará | Machado F.L.,Instituto Federal Of Educacao Ciencia E Tecnologia | da Costa J.M.C.,Federal University of Ceará
Revista Ciencia Agronomica | Year: 2014

The objective of this study was to evaluate the effect of the application of carnauba-based wax on the quality and conservation of the Orange cv. Valencia Delta produced in an area of semi-arid climate, during ambient storage (24 ± 2 °C and 40% ± 5 RH). The influence of the use of the coating on the physical properties (weight loss, peel moisture and flavedo colouration) and the physicochemical properties (soluble solids, titratable acidity, pH, soluble solids / titratable acidity ratio, ascorbic acid, yellow flavonoids and total polyphenols) was evaluated every four days for a period of 28 days. An increasing weight loss was seen during storage, the coated fruits however, lost weight at far lower rates than the control fruits. Accordingly, peel moisture decreased during storage, being more pronounced in the control fruits. The levels of soluble solids, titratable acidity, ascorbic acid, yellow flavonoids and total polyphenols increased, while the ratio of soluble solids to titratable acidity and the pH decreased during storage. The application of a carnauba-based coating prolonged the postharvest life of the 'Valencia Delta' orange grown in the state of Ceará, through a significant reduction in weight loss, enhancement in the brightness of the flavedo and maintenance of the physical and physicochemical properties during ambient storage.


Goncalves G.A.S.,Instituto Federal Of Educacao Ciencia E Tecnologia | de Barros Vilas Boas E.V.,Federal University of Lavras | de Resende J.V.,Federal University of Lavras | de Lima Machado A.L.,Federal University of Lavras | Boas B.M.V.,Instituto Federal Of Educacao Ciencia E Tecnologia
Ciencia e Agrotecnologia | Year: 2011

Caryocar brasiliense Camb. fruits is traditionally consumed cooked; and may suffer influences from this process. Thus, the objective of this work was to evaluate the effect of different times of cooking on the quality of this fruit. The fruits from North of Minas Gerais (Brazil) were selected, washed, sanitized, peeled and submitted to different times of cooking (0, 5, 10, 15, 20, 25, 30, 35 and 40 minutes). The analyses were carried out at the internal mesocarp and in the cooking water. With the increase of cooking time, decrease of color, firmness and the minerals potassium, sodium, zinc and iron decreased in the fruit and were detected in the water. The copper increased in internal mesocarp and reduced in the water. Potassium, sodium, zinc and iron decreased in the fruit with increased time of cooking and were detected in the water. Copper increased in the internal mesocarp and decreased in water. Phosphorus, calcium, magnesium, manganese and sulfur were not influenced by cooking time. Vitamin C, total carotenoids and betacarotene decreased to the time of cooking and of these, only vitamin C was found in the cooking water. It was concluded that even with 40 minutes of cooking these fruits continues as an important source of nutrients.


Bertolde F.Z.,Instituto Federal Of Educacao Ciencia E Tecnologia | Almeida A.-A.F.,University Estadual Of Santa Cruz | Pirovani C.P.,University Estadual Of Santa Cruz
PLoS ONE | Year: 2014

Soil flooding causes changes in gene transcription, synthesis and degradation of proteins and cell metabolism. The main objective of this study was to understand the biological events of Theobroma cacao during soil flooding-induced stress, using the analyses of gene expression and activity of key enzymes involved in fermentation, as well as the identification of differentially expressed proteins by mass spectrometry in two contrasting genotypes for flooding tolerance (tolerant - TSA-792 and susceptible - TSH-774). Soil anoxia caused by flooding has led to changes in the expression pattern of genes associated with the biosynthesis of alcohol dehydrogenase (ADH), pyruvate decarboxylase (PDC) and lactate dehydrogenase (LDH) in leaves and roots of the two evaluated genotypes. Significant differences were observed between the enzyme activities of the two genotypes. Leaves and roots of the TSA-792 genotype showed higher ADH activity as compared to the TSH-774 genotype, whereas the activities of PDC and LDH have varied over the 96 h of soil flooding, being higher for TSA-792 genotype, at the initial stage, and TSH-774 genotype, at the final stage. Some of the identified proteins are those typical of the anaerobic metabolism-involved in glycolysis and alcoholic fermentation-and different proteins associated with photosynthesis, protein metabolism and oxidative stress. The ability to maintain glycolysis and induce fermentation was observed to play an important role in anoxia tolerance in cacao and may also serve to distinguish tolerant and susceptible genotypes in relation to this stressor. © 2014 Bertolde et al.


Almeida E.M.,Instituto Federal Of Educacao Ciencia E Tecnologia | Junior J.H.C.,Federal University of Mato Grosso | Finger Z.,Federal University of Mato Grosso
Ciencia Florestal | Year: 2010

The objective of this study was to evaluate the quantity of carbon in teak (Tectona grandis L.f.), with 0.5; 1.5; 2.5; 3.5 and 5.5 years of age, during one year. The teak evaluated belonged to the BRASTECA AGROFLORESTAL LTDA company, located in the municipality of Santo Antônio of Leverger, Mato Grosso, Brazil. The carbon stock for each age was assessed every 90 days by determining the amount of litter dry mass, organic carbon proportion in the soil, root density in the soil and the volume of the staff and crown in the three teak trees randomly selected for harvest. The assessment of the plant size, as height, diameter at chest height and crown projection, was carried out with twenty preserved plants at each age. The dry mass of the roots was determined from the relation between the soil volume and the density of roots of the three plants per age, by making four perforations per plant, with six samples for each, up to 0.90 m of depth. The carbon proportion in the soil and in the litter was obtained by age at each collection through laboratory analysis. The average carbon found per hectare was 122.5 t of C at 0.5 years; 104.3 t of C at 1.5 years; 180.8 t of C at 2.5 years; 303.1 t of C at 3.5 years and 322.3 t of C at 5.5 years, considering the numbers of plants per hectare at the different ages. Afterward, the average distribution of carbon per system component was found to be 90.8% in the soil, 5.04% to the aerial part, 3.04% in the roots and 1.21% in the litter.


Rodrigues-Silva C.,Federal University of Santa Catarina | Flores-Nunes F.,Federal University of Santa Catarina | Vernal J.I.,Federal University of Santa Catarina | Cargnin-Ferreira E.,Instituto Federal Of Educacao Ciencia E Tecnologia | Bainy A.C.D.,Federal University of Santa Catarina
Aquatic Toxicology | Year: 2015

Cytochrome P450 family (CYP) is a group of proteins virtually found in all living organisms. The main role of most CYPs is to metabolize endo and xenobiotics. Most of the studies on CYP have been carried out in mammals and other vertebrates, however recently a growing interest has been devoted to the identification of CYP isoforms in invertebrates. A gene belonging to the CYP sub-family, CYP356A1, was identified in sanitary sewage-exposed Pacific oysters, Crassostrea gigas. Through heterologous expression, we produced CYP356A1 purified protein and raised a mouse polyclonal antibody. Dot blot tests showed that oysters exposed in situ for 14 days to untreated urban effluent discharges had significantly higher levels of CYP356A1 in digestive gland. Using immunohistochemical techniques we observed that the lining epithelial cells of mantle, stomach and intestine showed a strong CYP356A1 staining, but the mucus and secretory cells were negative. Digestive diverticulum parenchyma and gills lining cells showed strong CYP356A1 reaction, while the filamentary rod (connective tissue) was negative. Free cells, as hemocytes and brown cells also showed CYP356A1 immunoreactions indicating the presence of biotransformation activity in these cells. Male germ cells at early stages expressed CYP356A1 but not sperm mature cells, suggesting that this protein could be involved in the male gonadal development. This study shows the use of a specific antibody to a mollusk CYP isoform and that this protein is inducible in oysters environmentally exposed to urban sewage effluents. © 2014 Elsevier B.V.


Cavalcante R.M.,Federal University of Ceará | Sousa F.W.,Instituto Federal Of Educacao Ciencia E Tecnologia | Sousa F.W.,Federal University of Ceará | Nascimento R.F.,Federal University of Ceará | And 2 more authors.
Science of the Total Environment | Year: 2012

We measured polycyclic aromatic hydrocarbons (PAHs) in bulk precipitation in the Fortaleza metropolitan area, Ceará, Brazil, for the first time. Because little information is available concerning PAHs in tropical climatic regions, we assessed their spatial distribution and possible sources and the influence of urban activities on the depositional fluxes of PAHs in bulk precipitation. The concentrations of individual and total PAHs (σPAHs) in bulk precipitation ranged from undetectable to 133.9ng.L-1 and from 202.6 to 674.8ng.L-1, respectively. The plume of highest concentrations was most intense in a zone with heavy automobile traffic and favorable topography for the concentration of emitted pollutants. The depositional fluxes of PAHs in bulk precipitation calculated in this study (undetectable to 0.87μg.m-2.month-1) are 4 to 27 times smaller than those reported from tourist sites and industrial and urban areas in the Northern Hemisphere. Diagnostic ratio analyses of PAH samples showed that the major source of emissions is gasoline exhaust, with a small percentage originating from diesel fuel. Contributions from coal and wood combustion were also found. Major economic activities appear to contribute to pollutant emissions. © 2011 Elsevier B.V.


PubMed | Federal University of Ceará and Instituto Federal Of Educacao Ciencia E Tecnologia
Type: | Journal: Brazilian journal of biology = Revista brasleira de biologia | Year: 2015

AbstractThe mangrove oyster Crassostrea rhizophorae is an estuarine resource exploited by riverside communities in Northeast Brazil. Despite its socioeconomic importance, studies on the health status of this bivalve are scanty in this region. The purpose of the present study was to investigate the presence of the protozoan Perkinsus sp. in C. rhizophorae collected in August and September 2011 in three estuaries of the septentrional Northeast, Brazil: Jaguaribe (Cear), Camurupim (Piau) and Carnaubeiras (Maranho) (n= 150 specimens/site). The samples were submitted to Rays fluid thioglycollate medium (RFTM), PCR and histology assays. The RFTM assay revealed spherical, blue or bluish-black hypnospores of the genus Perkinsus in 50 specimens (Jaguaribe= 17.3%, Camurupim= 5.3%, Carnaubeiras= 10.6%). The intensity of the infection ranged from very light (1-10 cells per slide) to severe (more than 40 cells in each of 10 fields of the slide) for Jaguaribe; very light for Camurupim and very light to moderate (at least 40 cells observed in each of 10 fields of the slide) for Carnaubeiras. When submitted to confirmatory PCR analysis, 6 cases were confirmed (Jaguaribe=3, Camurupim=1, Carnaubeiras=2). The histology confirmed 21 cases of infection in specimens from the three estuaries. Although local collectors have reported no mortality in oyster populations that might be attributed to infection by Perkinsus, health surveillance of oyster populations in the septentrional region of Northeast Brazil is advisable.

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