Cytotoxicity of Agaricus sylvaticus in non-tumor cells (NTH/3T3) and tumor (OSCC-3) using tetrazolium (MTT) assay [Citotoxicidad de Agaricus sylvaticus en células no tumorales (NIH/3T3) y el tumor (CCCA-3) usando tetrazolio (MTT)]
Orsine J.V.C.,Instituto Federal Goiano Campus Urutai |
Brito L.M.,Estudante do Curso de Medicina |
Silva R.C.,University of Brasilia |
Almeida M.F.M.S.,University of Brasilia |
Novaes M.R.C.G.,University of Brasilia
Nutricion Hospitalaria | Year: 2013
The purpose of this study was to assess the cytotoxic effect of the non-fractionated aqueous extract of A. sylvaticus mushroom in cultures of non-tumor cells (NIH3T3) and tumor cells (OSCC-3). The cells were maintained in DMEN cell culture medium added of 10% of fetal bovine serum and 1% antibiotic. For the cytotoxicity test we prepared the aqueous mushroom extract at concentrations of 0.01 mg.ml-1, 0.02 mg.ml-1, 0.04 mg.ml-1, 0.08 mg.ml-1, 0.16 mg.ml-1, and 0.32 mg.ml-1. For the culture, 2 x 105 cells/ml was deposited in 96-well microplates during 24 hour incubation with subsequent exchange of medium by another containing the mushroom concentrations. After 24 hour incubation the medium was discarded and 100 ml of tetrazolium blue (MTT) was added at a concentration of 5 mg.ml-1. The microplates were incubated for 2 h at 37° C. Spectrophotometric analysis was performed using 570 nm wavelength. From the values of the optical densities we determined the drug concentration capable of reducing cell viability by 50%. Therefore, the mushroom A. sylvaticus, at all concentrations tested, did not show cytotoxic effects, once the inhibitory concentration (IC50) obtained for tumor cells OSCC-3 was 0.06194 mg.ml-1, and the IC50 checked for non-tumor cells NIH3T3 was 0,06468 mg.ml-1. This test made it possible to determine that A. sylvaticus mushroom has no cytotoxic effects, suggesting its use safe for human consumption.
Jesus F.G.,Instituto Federal Goiano Campus Urutai |
Nogueira L.,Instituto Federal Goiano Campus Urutai |
Boica Junior A.L.,São Paulo State University |
Ribeiro Z.A.,São Paulo State University |
And 2 more authors.
Australian Journal of Crop Science | Year: 2015
Eucalyptus brown looper Thyrinteina arnobia (Stoll, 1782) (Lepidoptera: Geometridae) is a major lepidopteran defoliator of eucalyptus. The objective of this study was to screen various eucalyptus genotypes and identify sources of resistance to T. arnobia. Leaves of the Corymbia citriodora, Eucalyptus alba, E. brassiana, E. camaldulensis, E. cloeziana, E. dunnii, E. grandis, E. pellita x E. tereticornis, E. resinifera, E. robusta, E. saligna, E. tereticornis, E. torelliana x C. citriodora and E. urophylla genotypes were provided to T. arnobia to assess antibiosis. The duration of the larval and pupal stages (days), pupal weight (mg), pupal and larval viability (%), adult longevity (days), sex ratio and total life cycle (days) were recorded. E. grandis, E. robusta, E. brassiana, E. tereticornis and E. saligna were found to be highly susceptible to T. arnobia, whereas E. torelliana x C. citriodora and E. dunnii showed antibiosis and/or antixenosis. These findings can assist growers in the management of T. arnobia and may be useful in the breeding of eucalyptus resistant to this insect.
Demite P.R.,Instituto Federal Goiano Campus Urutai |
Cavalcante A.C.C.,Instituto Federal Goiano Campus Urutai |
Dias M.A.,Instituto Federal Goiano Campus Urutai |
Lofego A.C.,Paulista University
International Journal of Acarology | Year: 2016
A new species Euseius uai sp. n. Demite & Lofego is described from the Minas Gerais State, Brazil. Euseius errabundus De Leon is recorded for the first time in Brazil and E. unisetus Moraes & McMurtry is rediscovered. Both were collected in Goiás State, Brazil. A dichotomous key for the Brazilian Euseius species is presented. © 2016 Informa UK Limited, trading as Taylor & Francis Group
Oliveira F.C.,Instituto Federal Goiano Campus Urutai |
De Sousa Netto M.S.,Instituto Federal Goiano Campus Urutai |
Da Silva Araujo L.S.,Instituto Federal Goiano Campus Urutai |
De Sousa Almeida A.C.S.,Instituto Federal Goiano Campus Urutai |
And 2 more authors.
Revista Caatinga | Year: 2016
Nitrogen (N) is one of the nutrients that have the most significant effect on corn grain productivity increase. In order to evaluate the effect of sources of N topdressing and doses in the grown corn culture, in a conventional till system, it was installed an experiment in the city of Urutaí, Goiâs, in the 2012/13 crop, in clayey textured Red Latosol. The experimental design was a randomized complete block design with four replications, with the treatments arranged in a factorial 3 x 5, consisting of three sources (coated urea, urea and ammonium sulfate) and five doses (0, 60, 120, 180 and 240 kg ha-1) of N, topdressing, applied in the surface and in a single dose, when the corn plants were in V4 stage. The hybrid used was the P3646H. We evaluated the following characters: stem diameter, plant height, height of ear insertion, leaf N, number of ears per plant, ear length, number of rows per ear, number of grains per row, 100 grains mass and grain productivity. All characters have a response to nitrogen fertilization and only the height of ear insertion showed no significant difference between sources. The source coated urea showed the best results, indicating that its use may be compensatory, especially in favorable conditions for the NH3 volatilization, as the application of high doses of N in the surface. © 2016, Universidade Federal Rural do Semi-Arid. All rights reserved.
Costa J.V.,Instituto Federal Goiano Campus Urutai |
Garbi Novaes M.R.C.,University of Brasilia |
Asquieri E.R.,Federal University of Goais
Journal of Bioanalysis and Biomedicine | Year: 2011
The chemical characterization of Agaricus sylvaticus (A. sylvaticus) cultivated in Brazil is necessary to determine nutritional and pharmacological substances in order to guarantee its safe use as food or herbal medicine. The objective of this study was to determine the chemical composition and assess the antioxidant potential of A. sylvaticus fungi grown in Brazil. Through this study it was able to observe the rich chemical composition of A. sylvaticus, highlighting the variety and amount of minerals as well as the high protein content of this fungus. It was also observed the great antioxidant potential of the aqueous, alcoholic and ethereal A. sylvaticus mushroom extracts, emphasizing the alcoholic extract, which testifies the extraordinary benefits of this fungus in diet, since antioxidants prevent premature aging and various types of cancer as well. The composition of A. sylvaticus mushroom displayed differences when compared to the chemical composition of the same fungus in other studies and with other Agaricales fungi. © 2011 Costa JV, et al.
Sunflower production grown after soybeans, corn and palisade grass, with and without supplemental irrigation [Produção de girassol cultivado após soja, milho e capim-marandu, com e sem irrigação suplementar]
Schwerz T.,Instituto Federal Goiano Campus Urutai |
Jakelaitis A.,Instituto Federal Goiano Campus Rio Verde |
Teixeira M.B.,Instituto Federal Goiano Campus Rio Verde |
Soares F.A.L.,Instituto Federal Goiano Campus Rio Verde |
Tavares C.J.,Instituto Federal Goiano Campus Posse
Revista Brasileira de Engenharia Agricola e Ambiental | Year: 2015
The objective of this research was to evaluate the production of sunflower in the off-season, supplemented or not with irrigation and cultivated after the crops of soybeans, corn or palisade grass. The treatments were allocated in a randomized blocks design in split plot (2×3), the supplemental irrigation by center pivot and without irrigation in the plots, and in the subplots the crops predecessors: soybeans, corn and palisade grass, with four replications. The height, stem diameter, shoot dry weight, number of leaves per plant and leaf area were evaluated at 15, 30, 45, 60, 75 and 90 days after emergence of sunflower plants. Sunflower plants were tall under all cultivation conditions, without variability in the evolution of stem diameter and of number of leaves. Leaf area was less in sunflower plants grown under corn straw and palisade grass under non-irrigated conditions. No significant effects on yield components - mass of thousand seeds, head diameter and oil and grain yield were observed. After cultivation of soybean, sunflower plants produced greater dry mass of shoots and higher oil content in the seed compared to plants established under corn stalk. © 2015, Departamento de Engenharia Agricola - UFCG/Cnpq. All rights reserved.
da Costa Estrela D.,Federal University of Goais |
de Souza D.C.,Instituto Federal Goiano Campus Urutai |
de Souza J.M.,Instituto Federal Goiano Campus Urutai |
da Silva Castro A.L.,Instituto Federal Goiano Campus Urutai
Check List | Year: 2015
Brazilian mammal fauna is considered to be the richest of the Neotropical region. However, in several regions of the country, there are gaps in the knowledge of its mammal fauna. Thus, we conducted a survey of the medium and large-sized mammal species in the fragmented Cerrado region in the southeast area of the state of Goiás. Nonlinear transects were conducted in 28 sessions over 13 months using direct observation methods, indirect observation methods (scratches, footprints, feces and lairs) and camera trapping. Twenty-five species belonging to eight orders were recorded. The species richness estimated by the Jackknife 1 method was 24.89 (±1.61) species in the transects and 16.88 (±1.29) species in camera trap, with stabilization of the species accumulation curves. Among the recorded species, 5 were endangered at the national level, and three were globally endangered. The high species richness found in the area in addition to the presence of endangered species highlights the need for conservation measures for the study site. © 2015, Check List and Authors.
Chemical properties of an oxisol under No-Tillage system as affected by soil management and crop rotation [Atributos Químicos De Latossolo Sob Plantio Direto Afetados Pelo Manejo Do Solo E Rotação De Culturas]
Nascente A.S.,Embrapa Arroz e Feijao |
Da Silveira P.M.,Embrapa Arroz e Feijao |
Lobo M.,Jr. |
Santos G.G.,Federal University of Goais |
Da Cunha P.C.R.,Instituto Federal Goiano Campus Urutai
Revista Caatinga | Year: 2014
The aim of this study was to determine the effects of soil management systems in no-tillage system (NTS) and crop rotations and on soil chemical properties. The experiment was conducted for six con-secutive years, during which were made 12 crops (summer and winter). The experimental design was com-pletely randomized in a split plot scheme. Treatments included four soil management (plots): P1 = NTS fol-lowed annually for a plowing in the winter; P2 = NTS followed biennially for a plowing in the winter; P3 = NTS followed every three years for a plowing and P4 = continuous NTS. Subplots were crop rotation with crops in the summer and winter: R1 = millet-common bean-millet-common bean-rice-common bean-millet-common bean-millet-common bean-rice-common bean; R2 = Soybean-common bean- soybean-wheat-rice -soybean-common bean- soybean-wheat-rice-common bean; R3 = corn-common bean-corn-tomato-rice-common bean-corn-common bean-corn-tomato-rice-common bean; R4 = millet-soybean-common bean -rice-common bean-millet-soybean-common bean-rice-common bean; R5 = millet-common bean-corn-common bean-rice-common bean-millet-common bean-corn-common bean-rice-common bean; R6 = Soybean-common bean-corn –common bean-rice-soybean-corn-common bean-rice-common bean. Each rotation cycle of three years crop season was repeated twice. Systems of soil management and crop rotations significantly affected soil chemical properties. The chemical attributes Ca, Mg, organic matter, P, K, Mn and Zn concentrated in the top-soil regardless of the crop rotation used in the managements of less soil disturbance. The pH values were simi-lar during the 12 seasons in six years. The crop rotations used in different soil managements under NTS provid-ed the improvement of soil fertility with values of organic matter, P, K, Cu, Mn and Zn contents equal to or higher than the initial values. © 2014, Universidade Federal Rural do Semi-Arid. All rights reserved.
PubMed | Federal University of Goais, Instituto Federal Goiano Campus Urutai, Instituto Federal Goiano and Federal University of Rio Grande do Sul
Type: | Journal: Physiology & behavior | Year: 2015
Obesity and chronic stress have been considered important public health problems that affect millions of people worldwide. Our aim was to analyze the effect of obesity associated with chronic stress on neurobehavioral parameters in female rats, considering that the association of these syndromes can enhance the negative effects on homeostasis. The animals were distributed into standard diet (Std), standard diet+stress (Std+stress), cafeteria diet (Cafe), and cafeteria diet+stress (Cafe+stress) groups. The animals of groups Std and Std+stress were fed with rodent standard feed. Groups Cafe and Cafe+stress, additionally to the standard feed, were offered palatable and calorie-rich processed food and cola-type soft drink ad libitum. From the eighth experimental week, groups Std+stress and Cafe+stress were subjected to restraint chronic stress model (50 days). After the stress protocol, predictive anxiety (open-field and elevated plus-maze tests) and depression (forced swim) were applied. The cafeteria diet was effective in inducing obesity. The ratio locomotion in the central quadrants/total locomotion evaluated during the open field test was not indicative of anxiogenic or anxiolytic effect in the animals behavior. However, the elevated plus maze test showed that obese and stressed animals were prone to higher anxiety levels. In addition, the obese and stressed animals display less climbing behavior than all the other groups, which can be considered an indicator of depression-like behavior. Nevertheless, it is suggested that the mechanisms involved in effects of obesity associated with chronic stress be better investigated in female rats, considering the organic complexity related to these modern illnesses.
PubMed | Federal University of Goais and Instituto Federal Goiano Campus Urutai
Type: | Journal: Chemosphere | Year: 2016
The main aim of the present paper is to assess whether the parental generation exposure to such discharges could cause object recognition deficits in their offspring. Male and female C57Bl/6J mice were put to mate after they were exposed to 7.5% and 15% tannery effluents or water (control group), for 60 days. The male mice were withdrawn from the boxes after 15 days and the female mice remained exposed to the treatment during the gestation and lactation periods. The offspring were subjected to the object recognition test after weaning in order to assess possible cognition losses. The results of the analysis of the novel object recognition index found in the testing session (performed 1h after the training session) applied to offspring from different experimental groups appeared to be statistically different. The novel object recognition index of the offspring from female mice exposed to tannery effluents (7.5% and 15% groups) was lower than that of the control group, and it demonstrated object recognition deficit in the studied offspring. The present study is the first to report evidences that parental exposure to effluent of tannery (father and mother) can cause object recognition deficit in the offspring, which is related to problems in the central nervous system.