Simões Filho, Brazil
Simões Filho, Brazil

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Ribeiro E.M.D.O.,Federal University of Bahia | Lima L.S.,Instituto Federal da Bahia | David J.M.,Federal University of Bahia | Vale A.E.D.,Federal University of Bahia | And 2 more authors.
Phytochemistry Letters | Year: 2013

A new tropane alkaloid, named the 7β-acetoxy-3β,6β- dibenzoyloxytropane (1), was isolated from a methanol extract of Erythroxylum rimosum O.E. Schulz leaves. Other known compounds were detected, including quercetin, kaempferol-3-O-α-l-arabinofuranoside, (+)-catechin, epicatechin, quercetin-3-O-α-arabinofuranoside, quercetin-3-O-α- arabinopyranoside, quercetin-3-O-β-arabinopyranoside, quercetin-3-β- glucopyranoside, kaempferol, quercetin-3-O-β-galactopyranoside, β-sitosterol, α-amyrin, β-amyrin, and the ester derivatives of these two amyrins. Compound 1 exhibited weak inhibition of acetylcholinesterase. Structural identification was performed using IR, ESIHRMS and one- and two-dimensional NMR data analyses and confirmed by comparison with literature data. © 2013 Phytochemical Society of Europe.

Miranda W.,Instituto Federal da Bahia | Miranda W.,Federal University of Bahia | Carneiro S.,Federal University of Bahia | Pigozzo C.,Federal University of Bahia
Journal of Cosmology and Astroparticle Physics | Year: 2014

We investigate the observational effects of a quintessence model in an anisotropic spacetime. The anisotropic metric is a non-rotating particular case of a generalized Gödel's metric and is classified as Bianchi III. This metric is an exact solution of the Einstein-Klein-Gordon field equations with an anisotropic scalar field ψ, which is responsible for the anisotropy of the spacetime geometry. We test the model against observations of type Ia supernovae, analyzing the SDSS dataset calibrated with the MLCS2k2 fitter, and the results are compared to standard quintessence models with Ratra-Peebles potentials. We obtain a good agreement with observations, with best values for the matter and curvature density parameters ΩM = 0.29 and Ωk= 0.01 respectively. We conclude that present SNe Ia observations cannot, alone, distinguish a possible anisotropic axis in the cosmos. © 2014 IOP Publishing Ltd and Sissa Medialab srl .

De L.e. Silva M.,Federal University of Bahia | David J.P.,Federal University of Bahia | Silva L.C.R.C.,Federal University of Bahia | Santos R.A.F.,Federal University of Bahia | And 4 more authors.
Molecules | Year: 2012

Betulinic, ursolic and oleanolic acids isolated from the aerial parts of Eriope blanchetii (Lamiaceae) were subjected to different esterification reactions, yielding 12 C-3 position ester derivatives. All compounds were identified using spectroscopic techniques, such as IR, 1H-NMR and MS. The derivatives were further investigated for their antioxidant level, Artemia salina lethality and antimicrobial activity. © 2012 by the authors; licensee MDPI, Basel, Switzerland.

PubMed | University Estadual Of Santa Cruz, Instituto Federal da Bahia, Federal University of Recôncavo da Bahia, University of Sao Paulo and Embrapa Mandioca e Fruticultura
Type: | Journal: Scientific reports | Year: 2016

Two citrus rootstocks, Rangpur lime (RL) and Sunki Maravilha mandarin (SM), were analyzed either ungrafted or grafted with their reciprocal graft combinations or with shoot scions of two commercial citrus varieties: Valencia orange (VO) and Tahiti acid lime (TAL). All graft combinations were subjected to distinct watering regimes: well-watered, severe drought and rehydration. Growth and water relation parameters, gas exchange as well as sugar and hormone profiles were determined. Data indicated that RL adopted a dehydration avoidance strategy and maintained growth, whereas SM adopted a dehydration tolerance strategy focused on plant survival. Compared with RL, the leaves and roots of SM exhibited higher concentrations of abscisic acid and salicylic acid, which induced drought tolerance, and accumulation of carbohydrates such as trehalose and raffinose, which are important reactive oxygen species scavengers. SM rootstocks were able to transfer their survival strategy to the grafted shoot scions (RL, VO, TAL). Because of their contrasting survival strategies, RL reached the permanent wilting point more quickly than SM whereas SM recovered from prolonged droughts more efficiently than RL. This is one of the most complete studies of drought tolerance mechanisms in citrus crops and is the first to use reciprocal grafting to clarify scion/rootstock interactions.

Sales J.H.,State University of Santa Cruz | Suzuki A.T.,São Paulo State University | Santos G.S.,Federal University of Espirito Santo | Lima C.A.,Instituto Federal da Bahia
Few-Body Systems | Year: 2015

We calculate the correction to the propagator of two bosons up to the g4 order in the “cross-ladder” approximation in the light front. The hierarchy of the coupled equations describe the propagator of two bosons in various cases, including the “cross-ladder” approximation. Our results show that the contribution of the pair is small and is limited to a range of bound state. © 2015, Springer-Verlag Wien.

PubMed | Instituto Federal da Bahia, Embrapa Tabuleiros Costeiros and Federal University of Sergipe
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

Cambui (Myrciaria tenella O. Berg) is a native species from Brazil, which belongs to the family Myrtaceae. Molecular characterization is one of the most used tools for the study of the biotechnological potential of species because the diversity level between individuals can be inferred. Analysis of genetic diversity is fundamental to the direction of the strategies necessary to form and maintain a germplasm. This study aimed to evaluate the genetic diversity in a natural population of cambui using inter-simple sequence repeat (ISSR) molecular markers. The natural population, which provided the plant material, is found at the Private Reserve of Natural Heritage of Caju, which belongs to the experimental field of Embrapa Tabuleiros Costeiros, in the municipality of Itaporanga dAjuda, SE, Brazil. Young leaves of each individual were collected for DNA extraction and analysis of PCR-ISSR. Thirty primers were tested and the top 10 were selected. The use of these primers resulted in 71 fragments with 98.3% polymorphism. Similarity of individuals ranged between 0.30 and 0.92. The most similar individuals were C13 and C17 and the most distant were C1 and C41. Through UPGMA, six distinct groups were identified. This information may be used for conservation of these genetic resources, germplasm exchange, creation of germplasm bank and in future studies with this species.

PubMed | Instituto Federal da Bahia, Embrapa Tabuleiros Costeiros and Federal University of Sergipe
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

Mangaba (Hancornia speciosa Gomes) is found in areas of coastal tablelands in the Brazilian Northeast and Cerrado regions. This species has been subjected to habitat fragmentation that is mainly due to human activity, and requires conservation strategies. The aim of this study was to analyze the structure and inter- and intrapopulation genetic diversity of natural populations of H. speciosa Gomes using inter-simple sequence repeat (ISSR) molecular markers. A total of 155 individuals were sampled in 10 natural populations (ITA, PAC, IND, EST, BC, PIR, JAP, BG, NEO, and SANT) in the State of Sergipe, Brazil. Fifteen primers were used to generate 162 fragments with 100% polymorphism. Genetic analysis showed that the variability between populations (77%) was higher than within populations (23%). It was possible to identify five different groups by the unweighted pair group method with arithmetic mean and principal coordinate analysis, and only one individual (E10) remained isolated. Using ISSR markers it was possible to obtain a molecular profile of the populations evaluated, showing that these markers were effective and exhibited sufficient polymorphism to estimate the genetic variability of natural populations of H. speciosa Gomes.

Silveira S.T.,Institute Investigacion En Ciencias Of La Alimentacion Cial | Silveira S.T.,Instituto Federal da Bahia | Martinez-Maqueda D.,Institute Investigacion En Ciencias Of La Alimentacion Cial | Recio I.,Institute Investigacion En Ciencias Of La Alimentacion Cial | Hernandez-Ledesma B.,Institute Investigacion En Ciencias Of La Alimentacion Cial
Food Chemistry | Year: 2013

Dipeptidyl peptidase-IV (DPP-IV) is a serine protease involved in the degradation and inactivation of incretin hormones that act by stimulating glucose-dependent insulin secretion after meal ingestion. DPP-IV inhibitors have emerged as new and promising oral agents for the treatment of type 2 diabetes. The purpose of this study was to investigate the potential of β-lactoglobulin as natural source of DPP-IV inhibitory peptides. A whey protein concentrate rich in β-lactoglobulin was hydrolysed with trypsin and fractionated using a chromatographic separation at semipreparative scale. Two of the six collected fractions showed notable DPP-IV inhibitory activity. These fractions were analysed by HPLC coupled to tandem mass spectrometry (HPLC-MS/MS) to identify peptides responsible for the observed activity. The most potent fragment (IPAVF) corresponded to β-lactoglobulin f(78-82) which IC 50 value was 44.7 u.M. The results suggest that peptides derived from β-lactoglobulin would be beneficial ingredients of foods against type 2 diabetes. © 2013 Elsevier Ltd. All rights reserved.

PubMed | Laboratorio Of Biotecnologia Molecular, Instituto Federal da Bahia, Federal University of Sergipe and University of Campinas
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

Varronia curassavica Jacq. is a medicinal and aromatic plant from Brazil with significant economic importance. Studies on genetic diversity in active germplasm banks (AGB) are essential for conservation and breeding programs. The aim of this study was to analyze the genetic diversity of V. curassavica accessions of the AGB of Medicinal and Aromatic Plants of the Federal University of Sergipe (UFS), using inter-simple sequence repeat molecular markers. Twenty-four primers were tested, and 14 were polymorphic and informative, resulting in 149 bands with 97.98% polymorphism. The UPGMA dendrogram divided the accessions into Clusters I and II. Jaccard similarity coefficients for pair-wise comparisons of accessions ranged between 0.24 and 0.78. The pairs of accessions VCUR-001/VCUR-503, VCUR-001/VCUR-504, and VCUR-104/VCUR-501 showed relatively low similarity (0.24), and the pair of accessions VCUR-402/VCUR- 403 showed medium similarity (0.78). Twenty-eight accessions were divided into three distinct clusters, according to the STRUCTURE analysis. The genetic diversity of V. curassavica in the AGB of UFS is low to medium, and it requires expansion. Accession VCUR-802 is the most suitable for selection in breeding program of this species, since it clearly represents all of the diversity present in the AGB.

This text aims, based on the literature on Physics Teaching Research, in showing that the Harvard Project, which has not been introduced in Brazil yet, contributed to the knowledge and insertion of the contextual ap- proach in Physics Teaching in Brazil, by influencing national curriculum projects to the mentioned teaching or helping the continuing education of our teachers and professors. © Sociedade Brasileira de Fisica. Printed in Brazil.

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