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Martinez-Fierro M.L.,Autonomous University of Zacatecas | Martinez-Fierro M.L.,Grupo Mexico | Perez-Favila A.,Autonomous University of Zacatecas | Garza-Veloz I.,Autonomous University of Zacatecas | And 18 more authors.
Biomarkers | Year: 2017

Background: Preeclampsia, a pregnancy disorder characterized by hypertension and proteinuria, represents the leading cause of fetal and maternal morbidity and mortality in developing countries. The identification of novel and accurate biomarkers that are predictive of preeclampsia is necessary to improve the prognosis of patients with preeclampsia. Objective: The objective of this study is to evaluate the usefulness of nine urinary metalloproteinases to predict the risk of preeclampsia development. Methods: MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-12 and MMP-13 were analyzed in urine (early-pregnancy) from 17 women predicted to develop preeclampsia and 48 controls using the Bio-Plex Pro-Human MMP panel (Bio-Rad, Hercules, CA). Results: Urinary MMP-2 showed differences between groups which allowed us to calculate an increased risk for PE development of up to 20 times among the study population. Conclusion: Increased urinary concentration of MMP-2 at 12 and 16 weeks of gestation predicted an increased risk of developing preeclampsia in the study population. © 2017 Informa UK Limited, trading as Taylor & Francis Group


Murillo-Zamora E.,Instituto Mexicano del Seguro Social | Garcia-Ceballos R.,Coordinacio n de Vigilancia Epidemiolo gica | Delgado-Enciso I.,University of Colima | Delgado-Enciso I.,Instituto Estatal Of Cancerologia | And 5 more authors.
Global Health Action | Year: 2016

Background: Excess body weight has become a major public health problem worldwide, and the burden of overweight and obesity was calculated in this work from a health economics perspective. Objective: To estimate the burden of disease attributable to overweight and obesity among males and females aged 20 years and older using years of life lost (YLL) and age-standardized YLL rates (ASYLL), and to rank the leading causes of premature death. Design: A cross-sectional study took place (2010-2014) and 6,054 deaths were analyzed. Thirteen basic causes of death associated with overweight or obesity were included. The population attributable fraction (PAF), YLL, and ASYLL were calculated. Results: The overall burden attributable to overweight and obesity was 36,087 YLL, and the estimated ASYLL per 10,000 persons was 1,098 and 1,029 in males and females, respectively. Type 2 diabetes mellitus was the main cause of premature death (males, 968 ASYLL; females, 772 ASYLL). Conclusions: Overweight and obesity are major risk factors of chronic diseases that are main causes of premature death in the study population. Strategies for preventing overweight and obesity may decrease the incidence and mortality associated with these non-communicable diseases. ASYLL seems to be an indicator that is particularly well adapted to decision-making in public health. © 2016 Efrén Murillo-Zamora et al.


Delgado-Enciso I.,University of Colima | Delgado-Enciso I.,Instituto Estatal Of Cancerologia | Soriano-Hernandez A.D.,University of Colima | Rodriguez-Hernandez A.,University of Colima | And 11 more authors.
International Braz J Urol | Year: 2015

Meclofenamic acid is a nonsteroidal anti-inflammatory drug that has shown therapeutic potential for different types of cancers, including androgen-independent prostate neoplasms. The antitumor effect of diverse nonsteroidal anti-inflammatory drugs has been shown to be accompanied by histological and molecular changes that are responsible for this beneficial effect. The objective of the present work was to analyze the histological changes caused by meclofenamic acid in androgen-independent prostate cancer. Tumors were created in a nude mouse model using PC3 cancerous human cells. Meclofenamic acid (10 mg/kg/day; experimental group, n=5) or saline solution (control group, n=5) was administered intraperitoneally for twenty days. Histological analysis was then carried out on the tumors, describing changes in the cellular architecture, fibrosis, and quantification of cellular proliferation and tumor vasculature. Meclofenamic acid causes histological changes that indicate less tumor aggression (less hypercellularity, fewer atypical mitoses, and fewer nuclear polymorphisms), an increase in fibrosis, and reduced cellular proliferation and tumor vascularity. Further studies are needed to evaluate the molecular changes that cause the beneficial and therapeutic effects of meclofenamic acid in androgen-independent prostate cancer.


Garcia-Rivera A.,University of Colima | Madrigal-Perez V.M.,University of Colima | Rodriguez-Hernandez A.,University of Colima | Martinez-Martinez R.,University of Colima | And 13 more authors.
Asian Journal of Animal and Veterinary Advances | Year: 2014

Recent studies have demonstrated a clinical relation between steatohepatitis and atherosclerosis. Both pathologies are of much interest due to the great morbidity and mortality associated with them and studies in animal models are necessary in order to find new therapies. The aim of the present study was to establish a new model for the simultaneous study of preclinical atherosclerosis and non-alcoholic steatohepatitis, in which BALB/c mice were fed a high-fat canine puppy diet. An experimental study was carried out on 3 BALB/c mice groups that were fed a standard diet (SDiet), a commercial atherogenic diet (AtheroDiet), or a canine puppy diet (CanDiet), for 6 months. A biochemical serum test and an intraperitoneal glucose tolerance test were carried out. The animals were euthanized at the sixth month and histopathologic slices of the liver and the thoracic and abdominal aorta were stained with hematoxylin and eosin or Masson's Trichrome. Steatohepatitis was evaluated and the thoracic and abdominal aortic intima-media thickness was measured. The ANOVA test was used for the group comparison. Steatosis and inflammation of the liver and thoracic and abdominal aortic intima-media thickness were all significantly increased in the AtheroDiet and CanDiet groups, compared with the SDiet group (p = 0.000). There was a positive correlation between steatohepatitis and the aortic intima-media thickness. No significant differences were found among the groups in relation to serum cholesterol or triglyceride levels; however, there was an alteration in the glucose tolerance curve in the AtheroDiet and CanDiet groups with respect to the SDiet group. There was no evidence of hepatic fibrosis in any of the groups. In conclusion, we were able to create a low-cost and accessible murine model for the simultaneous study of steatohepatitis and preclinical atherosclerosis. © 2014 Academic Journals Inc.


Martinez-Fierro M.L.,Molecular Medicine Laboratory | Reyes-Oliva E.A.,Molecular Medicine Laboratory | Cabral-Pacheco G.A.,Molecular Medicine Laboratory | Garza-Veloz I.,Molecular Medicine Laboratory | And 11 more authors.
Hypertension in Pregnancy | Year: 2016

Background: Inadequate trophoblast invasion and the subsequent inflammatory response have been implicated in preeclampsia (PE) pathogenesis. Because MYC-induced nuclear antigen (MINA) gene expression is involved in cell proliferation and differentiation, inflammatory response modulation, and the unpaired regulation of which is associated with human diseases, we sought to investigate the connection between MINA and PE. Objective: The aim of this study was to evaluate the possible relationship between the MINA rs4857304 variant and susceptibility to PE development as well as to estimate placental MINA gene expression and its association with PE. Methods: About 242 pregnant women (126 PE cases and 116 controls) were included. MINA genotyping and gene expression were evaluated by quantitative real-time polymerase chain reaction using TaqMan probes. Results: The G/G genotype of the MINA rs4857304 variant was associated with severe PE (p = 0.027, OR = 1.8, 95% CI = 1.8–3.2). Carriers of one G allele of the MINA rs4857304 variant exhibited a 1.7-fold increased risk of severe PE (p = 0.029, 95% CI = 1.1–3.0). MINA was underexpressed in preeclamptic placentas and MINA expression differed between the mild and severe PE groups. Differences in the expression levels of MINA were found among women with the T/T genotype of the rs4857304 polymorphism and carriers of at least one G allele (p = 0.024). Conclusion: PE and its severity are associated with the underexpression of placental MINA, and the G/G genotype of the MINA rs4857304 variant may modify the risk of severe PE among the PE cases evaluated. © 2016 Taylor & Francis.


Lopez-Hernandez Y.,Autonomous University of Zacatecas | Saldivar-Nava J.A.,Autonomous University of Zacatecas | Garza-Veloz I.,Autonomous University of Zacatecas | Garza-Veloz I.,Grupo Mexico | And 14 more authors.
International Urology and Nephrology | Year: 2016

Purpose: The aim of this study was to evaluate the usefulness of urine concentrations of 12 proteins as a risk parameter for developing preeclampsia (PE). Methods: A nested case–control study was designed to determine protein concentrations in urine from women predicted to develop PE (WPD-PE) and normotensive pregnancies (controls). Protein profiles were determined at 12, 16 and 20 gestational weeks (GW) using the Bio-Plex Pro human kidney toxicity Panel 1 and Panel 2 (Bio-Rad). Receiver operating characteristic (ROC) curve analyses were performed. Correlations between proteins and clinical parameters at the time of PE diagnosis were also assessed. Results: Significant differences were observed in urine cystatin C (Cys C) levels at 16 and 20 GW and clusterin at 20 GW between WPD-PE and controls (P < 0.05). ROC analysis revealed that Cys C at 16 GW had the highest area under the ROC curve (0.758). At 16 GW, patients with urine Cys C levels above 73.7 ng/mL had eightfold increased odds for developing PE (odds ratio 7.92; 95 % CI 1.3–47.5; P = 0.027). A positive correlation was found between urinary Cys C (at 16 and 20 GW) and leukocyte counts, total proteins, aspartate aminotransferase, alanine aminotransferase, bilirubin and lactate dehydrogenase at the time of PE diagnosis (P value < 0.05). Conclusions: Urinary Cys C and clusterin showed predictive value for PE development in our cohort. Further studies are needed to validate their use as predictive biomarkers for PE and/or their participation in PE pathogenesis. © 2016 Springer Science+Business Media Dordrecht


Soriano-Hernandez A.D.,Instituto Estatal Of Cancerologia | Soriano-Hernandez A.D.,University of Colima | Madrigal-Perez D.G.,Instituto Estatal Of Cancerologia | Madrigal-Perez D.G.,University of Colima | And 15 more authors.
Gynecologic and Obstetric Investigation | Year: 2015

Background/Aims: Breast cancer is the most common gynecologic malignancy known worldwide. The consumption of certain foods may modify the risk for its development. Peanuts and other seeds have shown anticarcinogenic effects in vitro, but there are a few studies that evaluate the effect of their consumption on the development of breast cancer. The aim of the present study was to determine whether there is an association between the consumption of peanuts, walnuts, and almonds and the development of breast cancer. Methods: We analyzed 97 patients presenting with breast cancer and 104 control subjects that did not have the pathology (BIRADS 1-2). An analysis of the main clinical characteristics and lifelong seed consumption was carried out. The association between the consumption of these foods and the risk for breast cancer was estimated by odds ratios and 95% confidence intervals, controlling other risk factors, using the Mantel-Haenszel analysis. Results: The high consumption of peanuts, walnuts, or almonds significantly reduced the risk for breast cancer by 2-3 times. This protective effect was not found with low or moderate seed consumption when compared with null consumption. Conclusions: High consumption of peanuts, walnuts, and almonds appears to be a protective factor for the development of breast cancer. © 2015 S. Karger AG, Basel.


Soriano-Hernandez A.D.,University of Colima | Galvan-Salazar H.R.,University of Colima | Montes-Galindo D.A.,University of Colima | Rodriguez-Hernandez A.,Research Center sarrollo En Ciencias Of La Salud | And 11 more authors.
International Urology and Nephrology | Year: 2012

Purpose Prostate cancer is a worldwide public health problem and its treatment continues to be a therapeutic challenge especially in patients with metastatic androgen-independent cancer. Inflammation is a process that has been involved in the origin of this cancer and its inhibition has been postulated as a prophylactic and therapeutic strategy. The present study evaluated two non-steroidal anti-inflammatory drugs (meclofenamic acid and mefenamic acid) that have been studied very little in regard to cancer treatment. Methods In vitro, the cytotoxic effects of meclofenamic acid and mefenamic acid were determined in human prostate cancer cell lines (LNCaP: androgendependent; and PC3: androgen-independent). In vivo trials were divided into two phases; meclofenamic acid toxicity was initially determined at different doses (0, 5, 10 and 20 mg/kg/day/25 days) in BALB/ c mice, after which a trial using non-toxic doses was carried out to evaluate the antitumor efficacy of the drug in a PC3/nude-mouse model of human androgen-independent prostate cancer. Results In vitro trials showed that only meclofenamic acid is highly cytotoxic in neoplastic prostate cells. The 5 and 10 mg/kg/day/25 day doses did not cause relevant toxicity in the BALB/c mouse trial, and so both doses were used in the nude-mouse model of cancer trial. This latter trial showed that meclofenamic acid significantly reduces tumor growth, prolongs survival, and is even capable of generating total tumor regression in up to 25% of mice treated at high dose. Conclusions Meclofenamic acid was shown to be a potential antineoplastic agent for both androgendependent and androgen-independent prostate cancer. © Springer Science+Business Media, B.V. 2011.


Delgado-Enciso I.,University of Colima | Delgado-Enciso I.,Instituto Estatal Of Cancerologia | Garcia-Rivera A.,University of Colima | Madrigal-Perez M.V.M.,University of Colima | And 15 more authors.
International Urology and Nephrology | Year: 2014

Introduction: A high-fat diet and male obesity are aspects associated with germinal epithelial alterations and male infertility. Some reports have shown that certain tetracyclines can protect the germinal epithelium from toxic drugs. The aim of the present study design was to evaluate the possible effect of doxycycline on testicular germ cells in individuals fed a Western diet (atherogenic), using a murine model. Methods: Two groups of male mice (BALB/c) were fed a high-fat Western diet (HFD). One of these two groups was given doxycycline at a dose of 10 mg/kg/day (HFD+Dox). A third group was fed a standard rodent diet (SD group). After 6 months, the mice were euthanized and morphologic and histopathologic analyses were performed. Results: Germinal epithelial height was similar between the SD group (54 μm) and the HFD+Dox group (53 μm) (p = 0.26), and it was significantly reduced in the HFD group (47 μm) (p = 0.0001). The degree of germinal epithelial loss (DGEL) was significantly lower in the SD (10) and HFD+Dox (12.5) groups than in the HFD group (30) (p = 0.0001 and =0.007, respectively). There were no differences in the DGEL between the SD and HFD+Dox groups (p = 0.42). Conclusions: Doxycycline administration was shown to prevent germinal epithelial loss in the testes of mice fed a high-fat diet. Future studies are necessary to evaluate the clinical usefulness of doxycycline or its analogs in persons with a habitual high-fat diet. © Springer Science+Business Media 2013.

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