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Soriano-Hernandez A.D.,University of Colima | Galvan-Salazar H.R.,University of Colima | Montes-Galindo D.A.,University of Colima | Rodriguez-Hernandez A.,Research Center sarrollo En Ciencias Of La Salud | And 10 more authors.
International Urology and Nephrology | Year: 2012

Purpose Prostate cancer is a worldwide public health problem and its treatment continues to be a therapeutic challenge especially in patients with metastatic androgen-independent cancer. Inflammation is a process that has been involved in the origin of this cancer and its inhibition has been postulated as a prophylactic and therapeutic strategy. The present study evaluated two non-steroidal anti-inflammatory drugs (meclofenamic acid and mefenamic acid) that have been studied very little in regard to cancer treatment. Methods In vitro, the cytotoxic effects of meclofenamic acid and mefenamic acid were determined in human prostate cancer cell lines (LNCaP: androgendependent; and PC3: androgen-independent). In vivo trials were divided into two phases; meclofenamic acid toxicity was initially determined at different doses (0, 5, 10 and 20 mg/kg/day/25 days) in BALB/ c mice, after which a trial using non-toxic doses was carried out to evaluate the antitumor efficacy of the drug in a PC3/nude-mouse model of human androgen-independent prostate cancer. Results In vitro trials showed that only meclofenamic acid is highly cytotoxic in neoplastic prostate cells. The 5 and 10 mg/kg/day/25 day doses did not cause relevant toxicity in the BALB/c mouse trial, and so both doses were used in the nude-mouse model of cancer trial. This latter trial showed that meclofenamic acid significantly reduces tumor growth, prolongs survival, and is even capable of generating total tumor regression in up to 25% of mice treated at high dose. Conclusions Meclofenamic acid was shown to be a potential antineoplastic agent for both androgendependent and androgen-independent prostate cancer. © Springer Science+Business Media, B.V. 2011. Source


Garcia-Rivera A.,University of Colima | Madrigal-Perez V.M.,University of Colima | Rodriguez-Hernandez A.,University of Colima | Martinez-Martinez R.,University of Colima | And 13 more authors.
Asian Journal of Animal and Veterinary Advances | Year: 2014

Recent studies have demonstrated a clinical relation between steatohepatitis and atherosclerosis. Both pathologies are of much interest due to the great morbidity and mortality associated with them and studies in animal models are necessary in order to find new therapies. The aim of the present study was to establish a new model for the simultaneous study of preclinical atherosclerosis and non-alcoholic steatohepatitis, in which BALB/c mice were fed a high-fat canine puppy diet. An experimental study was carried out on 3 BALB/c mice groups that were fed a standard diet (SDiet), a commercial atherogenic diet (AtheroDiet), or a canine puppy diet (CanDiet), for 6 months. A biochemical serum test and an intraperitoneal glucose tolerance test were carried out. The animals were euthanized at the sixth month and histopathologic slices of the liver and the thoracic and abdominal aorta were stained with hematoxylin and eosin or Masson's Trichrome. Steatohepatitis was evaluated and the thoracic and abdominal aortic intima-media thickness was measured. The ANOVA test was used for the group comparison. Steatosis and inflammation of the liver and thoracic and abdominal aortic intima-media thickness were all significantly increased in the AtheroDiet and CanDiet groups, compared with the SDiet group (p = 0.000). There was a positive correlation between steatohepatitis and the aortic intima-media thickness. No significant differences were found among the groups in relation to serum cholesterol or triglyceride levels; however, there was an alteration in the glucose tolerance curve in the AtheroDiet and CanDiet groups with respect to the SDiet group. There was no evidence of hepatic fibrosis in any of the groups. In conclusion, we were able to create a low-cost and accessible murine model for the simultaneous study of steatohepatitis and preclinical atherosclerosis. © 2014 Academic Journals Inc. Source


Delgado-Enciso I.,University of Colima | Delgado-Enciso I.,Instituto Estatal Of Cancerologia | Soriano-Hernandez A.D.,University of Colima | Rodriguez-Hernandez A.,University of Colima | And 10 more authors.
International Braz J Urol | Year: 2015

Meclofenamic acid is a nonsteroidal anti-inflammatory drug that has shown therapeutic potential for different types of cancers, including androgen-independent prostate neoplasms. The antitumor effect of diverse nonsteroidal anti-inflammatory drugs has been shown to be accompanied by histological and molecular changes that are responsible for this beneficial effect. The objective of the present work was to analyze the histological changes caused by meclofenamic acid in androgen-independent prostate cancer. Tumors were created in a nude mouse model using PC3 cancerous human cells. Meclofenamic acid (10 mg/kg/day; experimental group, n=5) or saline solution (control group, n=5) was administered intraperitoneally for twenty days. Histological analysis was then carried out on the tumors, describing changes in the cellular architecture, fibrosis, and quantification of cellular proliferation and tumor vasculature. Meclofenamic acid causes histological changes that indicate less tumor aggression (less hypercellularity, fewer atypical mitoses, and fewer nuclear polymorphisms), an increase in fibrosis, and reduced cellular proliferation and tumor vascularity. Further studies are needed to evaluate the molecular changes that cause the beneficial and therapeutic effects of meclofenamic acid in androgen-independent prostate cancer. Source


Soriano-Hernandez A.D.,Instituto Estatal Of Cancerologia | Soriano-Hernandez A.D.,University of Colima | Madrigal-Perez D.G.,Instituto Estatal Of Cancerologia | Madrigal-Perez D.G.,University of Colima | And 15 more authors.
Gynecologic and Obstetric Investigation | Year: 2015

Background/Aims: Breast cancer is the most common gynecologic malignancy known worldwide. The consumption of certain foods may modify the risk for its development. Peanuts and other seeds have shown anticarcinogenic effects in vitro, but there are a few studies that evaluate the effect of their consumption on the development of breast cancer. The aim of the present study was to determine whether there is an association between the consumption of peanuts, walnuts, and almonds and the development of breast cancer. Methods: We analyzed 97 patients presenting with breast cancer and 104 control subjects that did not have the pathology (BIRADS 1-2). An analysis of the main clinical characteristics and lifelong seed consumption was carried out. The association between the consumption of these foods and the risk for breast cancer was estimated by odds ratios and 95% confidence intervals, controlling other risk factors, using the Mantel-Haenszel analysis. Results: The high consumption of peanuts, walnuts, or almonds significantly reduced the risk for breast cancer by 2-3 times. This protective effect was not found with low or moderate seed consumption when compared with null consumption. Conclusions: High consumption of peanuts, walnuts, and almonds appears to be a protective factor for the development of breast cancer. © 2015 S. Karger AG, Basel. Source


Delgado-Enciso I.,University of Colima | Delgado-Enciso I.,Instituto Estatal Of Cancerologia | Garcia-Rivera A.,University of Colima | Madrigal-Perez M.V.M.,University of Colima | And 15 more authors.
International Urology and Nephrology | Year: 2014

Introduction: A high-fat diet and male obesity are aspects associated with germinal epithelial alterations and male infertility. Some reports have shown that certain tetracyclines can protect the germinal epithelium from toxic drugs. The aim of the present study design was to evaluate the possible effect of doxycycline on testicular germ cells in individuals fed a Western diet (atherogenic), using a murine model. Methods: Two groups of male mice (BALB/c) were fed a high-fat Western diet (HFD). One of these two groups was given doxycycline at a dose of 10 mg/kg/day (HFD+Dox). A third group was fed a standard rodent diet (SD group). After 6 months, the mice were euthanized and morphologic and histopathologic analyses were performed. Results: Germinal epithelial height was similar between the SD group (54 μm) and the HFD+Dox group (53 μm) (p = 0.26), and it was significantly reduced in the HFD group (47 μm) (p = 0.0001). The degree of germinal epithelial loss (DGEL) was significantly lower in the SD (10) and HFD+Dox (12.5) groups than in the HFD group (30) (p = 0.0001 and =0.007, respectively). There were no differences in the DGEL between the SD and HFD+Dox groups (p = 0.42). Conclusions: Doxycycline administration was shown to prevent germinal epithelial loss in the testes of mice fed a high-fat diet. Future studies are necessary to evaluate the clinical usefulness of doxycycline or its analogs in persons with a habitual high-fat diet. © Springer Science+Business Media 2013. Source

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