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Gasteiz / Vitoria, Spain

Anitua E.,Implantology and Oral Rehabilitation | Anitua E.,BTI Biotechnology Institute | Tejero R.,BTI Biotechnology Institute | Alkhraisat M.H.,BTI Biotechnology Institute | And 4 more authors.
BioDrugs | Year: 2013

Growth factors and cytokines are active players in controlling the different stages of wound healing and tissue regeneration. Recent trends in personalized regenerative medicine involve using patient's own platelet-rich plasma for stimulating wound healing and tissue regeneration. This technology provides a complex cocktail of growth factors and even a fibrin scaffold with multiple biologic effects. In the last few years, an increasing number of studies provide evidence of the potential of combining platelet-rich plasma with different biomaterials in order to improve their properties, including handling, administration, bioactivity, and level of osseointegration, among others. In this review, we discuss the use of platelet-rich plasma as an alternative, easy, cost-effective, and controllable strategy for the release of high concentrations of many endogenous growth factors. Additionally, we provide an overview of the current progress and future directions of research combining different types of biomaterials with platelet-rich plasma in tissue engineering and regenerative medicine. © 2012 Springer International Publishing Switzerland.

Anitua E.,Instituto Eduardo Anitua | Muruzabal F.,Instituto Eduardo Anitua | Pino A.,Instituto Eduardo Anitua | Merayo-Lloves J.,Instituto Oftalmologico Fernandez Vega | Orive G.,Instituto Eduardo Anitua
Cornea | Year: 2013

Purpose: We evaluated whether plasma rich in growth factors eye drops maintain their composition and biological activity after storage for 3 months at 220°C and after storage at 4°C or room temperature (RT) for 24 hours, compared with samples obtained at time 0 (fresh samples). Methods: Blood from 10 healthy donors was collected, centrifuged, and plasma rich in growth factors was prepared by avoiding the collection of the buffy coat. Eye drops were kept fresh or were stored at 220°C for 15, 30, and 90 days. For each time, 2 aliquots were kept at RT or at 4°C for 24 hours. Osmolarity, vitamin A, fibronectin, plateletderived growth factor-AB, vascular endothelial growth factor, epithelial growth factor, and transforming growth factor-β1 were quantified. The proliferative and migratory potential of the eye drops was assayed on primary human keratocytes. Results: Platelet-derived growth factor-AB, vascular endothelial growth factor, epithelial growth factor, and vitamin A levels remained constant for each time and for each storage condition, whereas fibronectin, transforming growth factor-β1, and osmolarity values were slightly modified after freezing. Cell proliferation and migration were significantly enhanced with the biological eye drops independently of the time and the storage condition. No microbial contamination was observed in any plasma rich in growth factors eye drops. Conclusions: Plasma rich in growth factors eye drops can be stored for up to 3 months without any reduction of the main proteins involved in ocular surface healing. Their use during 24 hours either at 4°C or at RT did not alter the composition and the in vitro biological activity of the eye drops. Copyrigt © 2013 Lippincott Williams & Wilkins.

Orcajo B.,Clinica Juber | Muruzabal F.,Instituto Eduardo Anitua | Isasmendi M.C.,Clinica Juber | Gutierrez N.,Clinica Juber | And 2 more authors.
Diabetes Research and Clinical Practice | Year: 2011

A 71 year old person with diabetes with a severe mal perforant ulcer in the right foot was treated twice with autologous plasma-rich in growth factors (PRGF) obtained from her own blood. After PRGF treatment the severe mal perforant ulcer completely healed in 10 weeks. © 2011 Elsevier Ireland Ltd.

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