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German-Ramirez E.,Florida A&M University | Kairo M.T.K.,University of Maryland Eastern Shore | Haseeb M.,Florida A&M University | Serra C.A.,Instituto Dominicano Of Investigaciones Agropecuarias Y Forestales Idiaf
Florida Entomologist | Year: 2014

The mealybug Hypogeococcus pungens Granara de Willink (Hemiptera: Pseudococcidae) is a new record for the Dominican Republic, based on specimens collected on 21 May 2010 from the ornamental plant Gomphrena globosa L. (Caryophyllales: Amaranthaceae), in Santo Domingo.

Matos L.A.,University of Florida | Matos L.A.,Instituto Dominicano Of Investigaciones Agropecuarias Y Forestales Idiaf | Hilf M.E.,U.S. Department of Agriculture | Cayetano X.A.,IDIAF | And 2 more authors.
Plant Disease | Year: 2013

Citrus tristeza virus (CTV) is the most destructive viral pathogen of citrus and has been an important concern for the citrus industry in the Dominican Republic. Earlier studies documented widespread distribution of mild isolates of the T30 genotype, which caused no disease in the infected trees, and a low incidence of isolates of the VT and T3 genotypes, which were associated with economically damaging decline and stem-pitting symptoms in sweet orange and Persian lime, the two major citrus varieties grown in the Dominican Republic. In light of the dramatic increase in the number of severely diseased citrus trees throughout the country over the last decade, suggesting that field populations of CTV have changed, we examined the CTV pathosystem in the Dominican Republic to assess the dynamics of virus populations. In this work, we characterized the molecular composition of 163 CTV isolates from different citrus-growing regions. Our data demonstrate a dramatic change in CTV populations, with the VT genotype now widely disseminated throughout the different regions and with the presence of two new virus genotypes, T36 and RB. Multiple infections of trees resulted in development of complex virus populations composed of different genotypes. © 2013 The American Phytopathological Society.

Abo-Shnaf R.I.A.,University of Sao Paulo | Abo-Shnaf R.I.A.,Egyptian Plant Protection Res Institute | Sanchez L.,University of Sao Paulo | Sanchez L.,Instituto Dominicano Of Investigaciones Agropecuarias Y Forestales Idiaf | De Moraes G.J.,University of Sao Paulo
Systematic and Applied Acarology | Year: 2016

A survey of plant inhabiting mesostigmatid mites was recently conducted in the Dominican Republic. Phytoseiidae was by far the most represented family, with 12 species. Amblyseius tamatavensis Blommers was the most common species; it was reported from four provinces on five plant species. Two species of Ascidae, four of Blattisociidae and three of Melicharidae were also found. Four blattisociid species of the genus Lasioseius are here described for the first time, namely Lasioseius dominicensis n. sp., Lasioseius oryzae n. sp., Lasioseius prorsoperitrematus n. sp. and Lasioseius sanchezensis n. sp.. Morphological information about all other species collected is presented. © Systematic & Applied Acarology Society.

Martinez L.S.,Instituto Dominicano Of Investigaciones Agropecuarias Y Forestales Idiaf | Flechtmann C.H.W.,University of Sao Paulo | De Moraes G.J.,University of Sao Paulo
Zootaxa | Year: 2014

Fourteen mite species of plant-associated mites of the suborder Prostigmata are reported from the Dominican Republic. Four of these refer to new findings for the country, including Petrobia (Tetranychina) hispaniola n. sp. Sánchez & Flechtmann, described from specimens collected from leaves of Citrus sp. (Rutaceae) and Rosa sp. (Rosaceae). A key for the separation of the world species of Petrobia (Tetranychina) is presented. Copyright © 2014 Magnolia Press.

PubMed | Egyptian Plant Protection Res Institute, Retired scholar, Instituto Dominicano Of Investigaciones Agropecuarias Y Forestales Idiaf, University of Sao Paulo and Directorate of Research
Type: Journal Article | Journal: Zootaxa | Year: 2015

The Lasioseius phytoseioides species group was first characterised over 50 years ago. Two species of this group, Lasioseius chaudhrii (Wu & Wang) and Lasioseius parberlesei Bhattacharyya, have been considered potentially effective as biological control agents of pest mites of the family Tarsonemidae on rice (Oryza sativa L.) in Asia. A new characterisation of the species group is presented, taking into account a new species here described, Lasioseius piracicabensis Moraes & Prez-Madruga n. sp., as well as other species mostly described since the first characterisation of the group. The main characteristics of the included species are the reduced number of dorsal idiosomal setae, including the absence of j1 and z1; antiaxial surface of fixed cheliceral digit with a subterminal pointed process; and males with broad lateral expansion of the peritrematic shield in the region between coxae II-III, bearing a pore and a lyrifissure. Complementary notes are presented for Lasioseius annandalei Bhattacharyya & Bhattacharyya, Lasioseius chaudhrii (Wu & Wang), Lasioseius parberlesei Bhattacharyya, L. phytoseioides Chant, Lasioseius punjabensis Bhattacharyya & Sanyal, Lasioseius terrestris Menon & Ghai and Lasioseius youcefi Athias-Henriot. New synonymies are proposed and possible misidentifications in the literature are discussed. A dichotomous key is presented to help the identification of the species of the group, and the distribution of the species is summarised.

PubMed | University of Sao Paulo and Instituto Dominicano Of Investigaciones Agropecuarias Y Forestales Idiaf
Type: Journal Article | Journal: Zootaxa | Year: 2014

Fourteen mite species of plant-associated mites of the suborder Prostigmata are reported from the Dominican Republic. Four of these refer to new findings for the country, including Petrobia (Tetranychina) hispaniola n. sp. Snchez & Flechtmann, described from specimens collected from leaves of Citrus sp. (Rutaceae) and Rosa sp. (Rosaceae). A key for the separation of the world species of Petrobia (Tetranychina) is presented.

Lopez-Rodriguez G.,Instituto Dominicano Of Investigaciones Agropecuarias Y Forestales Idiaf | Sotomayor-Ramirez D.,University of Puerto Rico at San Juan | Amador J.A.,University of Rhode Island | Schroder E.C.,University of Puerto Rico at San Juan
Tropical Ecology | Year: 2015

Coffee (Coffea arabica L.) production is important for its economic, ecological and social values in tropical areas. Whether coffee is grown under shade (SHD) or full sunlight (SUN), may have a direct impact on soil nitrogen (N) cycling, which can affect yield and agroecosystem sustainability. We studied N cycling in coffee farms in three municipalities in Puerto Rico and evaluated three ecosystem types in each: SUN coffee, SHD coffee and secondary forest (FOR). Aboveground litter dry matter and litter N inputs were quantified. Litter dry matter inputs (t ha-1year-1) were higher in SHD (2.15) and FOR (1.83), and were significantly greater than SUN (1.40). Litter N inputs (kg N ha-1year-1) were significantly lower in SUN (31) than in SHD (52) and FOR (43). Cycling of N was evaluated in detail in the municipality of Las Marias in SHD and SUN coffee. Litter N inputs (kg N ha-1year-1) to soil were significantly different between FOR (41) and SHD (56). The standing stock of N in aboveground biomass SHD was ∼3 times that in SUN, and total N input was twice that in SUN. However, soil N standing stocks were similar in SHD and SUN, indicating faster litter N turnover in SUN than in SHD ecosystems. By contrast, net soil N mineralization rates (kg N ha-1year-1) were ∼2 times higher in SHD (96) than in SUN (49), indicating that soil N turnover is greater in SHD than SUN. Our results suggest that litter N is mineralized at a slower rate in SHD than in SUN, whereas soil N is mineralized at a slower rate in SUN than in SHD. Higher inputs of N to soil, and soil N turnover in SHD may result in improved coffee production and associated forest biomass N uptake. Higher soil N mineralization rates in SHD coffee suggest improved ecosystem sustainability than in SUN coffee, presumably due to higher microbial activity, greater microbial diversity and substrate availability. © International Society for Tropical Ecology.

Martinez R.T.,Instituto Dominicano Of Investigaciones Agropecuarias Y Forestales Idiaf | Poojari S.,Washington State University | Tolin S.A.,Virginia Polytechnic Institute and State University | Cayetano X.,Instituto Dominicano Of Investigaciones Agropecuarias Y Forestales Idiaf | Naidu R.A.,Washington State University
Plant Disease | Year: 2014

In the Dominican Republic, green bell pepper (Capsicum annuum L.) and tomato (Solanum lycopersicum L.) are widely cultivated under protected greenhouse conditions as high value commercial crops for export. For the past 2 to 3 years, pepper and tomato have been observed in protected crop facilities in Jarabacoa and Constanza in the North Region with chlorotic and necrotic spots and rings on leaves, petioles, and stems, leaf bronzing, and tip necrosis. Fruits on symptomatic pepper and tomato plants showed concentric rings, irregular chlorotic blotches and deformation, and uneven maturation and development. Incidence on pepper and tomato was 20 to 100% and 5 to 20%, respectively. In initial tests, leaves and fruits from each of 20 symptomatic tomato and pepper plants from several greenhouse facilities were reactive in Tomato spotted wilt virus (TSWV; genus Tospovirus, family Bunyaviridae) immunostrip assays (Agdia, Inc., Elkhart, IN). Since these immunostrips are known to react with other tospoviruses, such as Tomato chlorotic spot virus (TCSV) and Groundnut ring spot virus, additional molecular diagnostic assays were conducted. Leaf and fruit samples from symptomatic plants were imprinted on nitrocellulose membrane (NCM) (2), air-dried, and sent to Washington State University for confirmatory tests. Viral nucleic acids were eluted from NCM discs (1) and subjected to reverse transcription (RT)-PCR using primers gL3637 (CCTTTAACAGTDGAAACAT) and gL4435 (CATDGCRCAAGARTGRTARACAGA) designed to amplify a portion of the L RNA segment of several tospoviruses (3). A single DNA product of ~800 bp was amplified from all samples. Amplicons from two tomato (leaf and fruit) and one pepper fruit samples were cloned separately into pCR2.1 (Invitrogen Corp., Carlsbad, CA). Two independent clones per amplicon were sequenced in both orientations. Sequence analyses of these clones (GenBank Accession Nos. KF 219673 to 75) showed 100% nucleotide sequence identity among themselves and 97% identity with corresponding L RNA sequences of pepper isolates of TSWV from Taiwan (HM180088) and South Korea (HM581940), 94 to 95% with tomato isolates of TSWV from South Korea (HM581934) and Hawaii (AY070218), and 89% with a tomato isolate from Indonesia (FJ177301). These results further confirm the presence of TSWV in symptomatic tomato and pepper plants. A comparison of TSWV sequences from the Dominican Republic with TSWV isolates from the United States and other countries in the Caribbean region could not be made due to the absence of corresponding sequences of the L-RNA of the virus from these countries in GenBank. TSWV-positive samples were negative for TCSV in RT-PCR, indicating the absence of this tospovirus that has been reported in the Caribbean region (data not shown). To our knowledge, this is the first confirmed report of TSWV in tomatoes and peppers in the Dominican Republic. The presence of vector thrips, Frankliniella occidentalis, on symptomatic plants was also confirmed, suggesting a role in the spread of TSWV under greenhouse conditions. Recent surveys identified some greenhouses with 100% symptomatic peppers. The presence of TSWV in tomato and pepper has important implications for the domestic and export vegetable industry in the Dominican Republic because of the broad host range of the virus (4). It is critical for commercial producers to monitor TSWV and deploy appropriate management strategies to limit virus spread.

Boza E.J.,U.S. Department of Agriculture | Irish B.M.,U.S. Department of Agriculture | Meerow A.W.,U.S. Department of Agriculture | Tondo C.L.,U.S. Department of Agriculture | And 9 more authors.
Genetic Resources and Crop Evolution | Year: 2013

Cacao (Theobroma cacao L.) is a significant agricultural commodity in the Dominican Republic, which ranks 11th in the world for cacao exports. To estimate genetic diversity, determine genetic identity, and identify any labeling errors, 14 SSR markers were employed to fingerprint 955 trees among cacao germplasm accessions and local farmer selections (LFS). Comparisons of homonymous plants across plots revealed a significant misidentification rate estimated to be 40.9 % for germplasm accessions and 17.4 % for LFS. The 14 SSRs amplified a total of 117 alleles with a mean allelic richness of 8.36 alleles per locus and average polymorphism information content (PIC) value of 0.67 for the germplasm collection. Similar levels of variation were detected among the LFS where a total of 113 alleles were amplified with a mean of 8.07 alleles per locus and PIC of 0.57. The observed heterozygosity (Hobs) was 0.67 for the germplasm collection and 0.60 for LFS. Based on population structure analysis 43. 9 % of the germplasm accessions and 72.1 % of the LFS are predominantly of the Amelonado ancestry. Among these Amelonado, 51.7 % for the germplasm collection and 50.6 % for LFS corresponded to Trinitario hybrid lineage. Criollo ancestry was found in 7.6 and 9.5 % of the germplasm accessions and LFS, respectively. The Contamana, Nacional, and Iquitos backgrounds were also observed in both populations, but the Curaray background was only detected in the germplasm accessions. No Purús or Guiana ancestry was found in either of the populations. Overall, significant genetic diversity, which could be exploited in the Dominican Republic breeding and selection programs, was identified among the germplasm accessions and LFS. © 2012 Springer Science+Business Media B.V. (outside the USA).

Gonzalez N.,University of Nebraska - Lincoln | Godoy-Lutz G.,Instituto Dominicano Of Investigaciones Agropecuarias Y Forestales Idiaf | Steadman J.R.,University of Nebraska - Lincoln | Higgins R.,University of Nebraska - Lincoln | Eskridge K.M.,University of Nebraska - Lincoln
Journal of General Plant Pathology | Year: 2012

Web blight, an important foliar disease of dry beans in the Americas, is a challenge to manage. We studied genetic variation of 92 isolates of Rhizoctonia solani subgroups AG-1-IE and AG-1-IF using DNA fingerprinting methods and mycelial compatibility grouping. The isolates were collected over 13 years from bean fields in the Dominican Republic, Honduras and Puerto Rico. Cluster and AMOVA analysis of combined data from two universal rice primers and two internal sequence repeats revealed significant genetic variation among and within populations of both subgroups. Variation was influenced by geographic origin and sampling year for AG-1-IE isolates and geographic origin for AG-1-IF isolates. Mycelial compatibility of paired isolates was mostly scored as incompatible in both subgroups and supported many unique phenotypes. Only two isolates of AG-1-IE displayed mycelial compatibility and DNA fingerprints, suggesting clonal origin. Genetic variation in these AG-1-IE and AG-1-IF isolate populations may explain the lack of durable resistance to web blight reported in dry beans. © 2012 The Phytopathological Society of Japan and Springer.

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