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Garcia-Fraile P.,University of Salamanca | Garcia-Fraile P.,John Innes Center | Carro L.,University of Salamanca | Robledo M.,University of Salamanca | And 16 more authors.
PLoS ONE | Year: 2012

The biofertilization of crops with plant-growth-promoting microorganisms is currently considered as a healthy alternative to chemical fertilization. However, only microorganisms safe for humans can be used as biofertilizers, particularly in vegetables that are raw consumed, in order to avoid sanitary problems derived from the presence of pathogenic bacteria in the final products. In the present work we showed that Rhizobium strains colonize the roots of tomato and pepper plants promoting their growth in different production stages increasing yield and quality of seedlings and fruits. Our results confirmed those obtained in cereals and alimentary oil producing plants extending the number of non-legumes susceptible to be biofertilized with rhizobia to those whose fruits are raw consumed. This is a relevant conclusion since safety of rhizobia for human health has been demonstrated after several decades of legume inoculation ensuring that they are optimal bacteria for biofertilization. © 2012 Garcia-Fraile et al.


Abril D.,Catholic University of the Maule | Medina M.,Instituto Cubano Of Investigaciones Of Los Derivados Of La Cana Of Azucar | Abril A.,Instituto Cubano Of Investigaciones Of Los Derivados Of La Cana Of Azucar
Brazilian Journal of Chemical Engineering | Year: 2012

Results are presented on the hot water prehydrolysis of sugar cane bagasse for obtaining ethanol by fermentation. The experimental study consisted of the determination of the effect of temperature and time of prehydrolysis on the extraction of hemicelluloses, with the objective of selecting the best operating conditions that lead to increased yield of extraction with a low formation of inhibitors. The study, carried out in a pilot plant scale rotational digester, using a 3 2 experimental design at temperatures of 150-190°C and times of 60-90 min, showed that it is possible to perform the hot water prehydrolysis process between 180-190°C in times of 60-82 min, yielding concentrations of xylose ≥ 35 g/L, furfural ≤ 2.5 g/L, phenols from soluble lignin ≤ 1.5 g/L, and concentrations ≤ 3.0 g/L of hemicelluloses in the cellolignin residue. These parameters of temperature and prehydrolysis time could be used for the study of the later hydrolysis and fermentation stages of ethanol production from sugar cane bagasse.


Otero M.A.,Instituto Cubano Of Investigaciones Of Los Derivados Of La Cana Of Azucar | Guerrero I.,National University of Quilmes | Wagner J.R.,San Rafael Atlixco 186 | Cabello A.J.,Instituto Cubano Of Investigaciones Of Los Derivados Of La Cana Of Azucar | And 6 more authors.
Biotecnologia Aplicada | Year: 2011

In the last 200 years, and still today, yeast is well known for its application in brewing, alcohol fermentation and wine and bread making. They are an endless source of new food ingredients and additives with excellent functional and nutritional properties, now through the use of innovative elaboration and fractionation techniques that come mainly from biotechnology. The book reviewed here contains fourteen chapters in 246 pages that deal with yeasts employed as food ingredients and their potential as Nutraceutics. It compiles the expertise of three Latin American institutions that have given priority to the generation of basic knowledge on yeast and set the grounds for the development of new technologies based on these microorganisms. This is a sample of the alternatives offered by yeast in the fields of food science and technology.


Coelho L.F.,Paulista University | de Lima C.J.B.,Paulista University | Bernardo M.P.,Paulista University | Alvarez G.M.,Instituto Cubano Of Investigaciones Of Los Derivados Of La Cana Of Azucar | Contiero J.,Paulista University
Journal of the Science of Food and Agriculture | Year: 2010

BACKGROUND: L(+)-Lactic acid is used in the pharmaceutical, textile and food industries as well as in the synthesis of biodegradable plastics. The aim of this study was to investigate the effects of different medium components added in cassava wastewater for the production of L(+)-lactic acid by Lactobacillus rhamnosus B 103. RESULTS: The use of cassava wastewater (50 g L -1 of reducing sugar) with Tween 80 and corn steep liquor, at concentrations (v/v) of 1.27 mL L -1 and 65.4 mL L -1 respectively led to a lactic acid concentration of 41.65 g L -1 after 48 h of fermentation. The maximum lactic acid concentration produced in the reactor after 36 h of fermentation was 39.00 g L -1 using the same medium, but the pH was controlled by addition of 10 mol L -1 NaOH. CONCLUSION: The use of cassava wastewater for cultivation of L. rhamnosus is feasible, with a considerable production of lactic acid. Furthermore, it is an innovative proposal, as no references were found in the scientific literature on the use of this substrate for lactic acid production. © 2010 Society of Chemical Industry.


Mendoza S.,Federal University of Itajubá | Almazan O.,Instituto Cubano Of Investigaciones Of Los Derivados Of La Cana Of Azucar | Silva E.,Federal University of Itajubá | Cobas V.,Federal University of Itajubá | Guillen R.,University of the East of Cuba
World Renewable Energy Forum, WREF 2012, Including World Renewable Energy Congress XII and Colorado Renewable Energy Society (CRES) Annual Conferen | Year: 2012

For the first time a model structured according (Duffie J. and Beckman W., 1980), (Gaven H. and Bannerot R. 1984), (Kongtragool, B. and Wongwises S., 2005), is presented, that describes the behavior of an electric power generation system, based on a solar energy technology using a Dish/Stirling system coupled to an electric generator. The first part of the model determines the characteristic of the earth movement related to the Sun, obtaining the follow-up angles to maximize the temperature in the receiver at any geographical location. The efficiencies for every stage of the system were also calculated, based on the reports of the Meteorological Station of the University Itajubá-Brazil. Also, using the model, the optical-geometric variables were calculated, which allows optimizing the behavior of the system for any geometric dimensions and types of materials of the collector/receiver. In addition, the model gives the heat balance calculation evaluating the efficiencies of the manifold, the receiver, the Stirling motor and the whole system. Using MATLAB a simulation was done and the results validated by specialized publications. For a collector of 7,5 m of diameter, having a constant radiation of 1000 W/m2, the optimum temperature for the higher efficiency of the system (67%), was 1551 K. For the maximum power condition, the model shows that the temperature must be 1664 K, but with an overall efficiency of only 26 %.


Veana F.,Autonomous University of Coahuila | Martinez-Hernandez J.L.,Autonomous University of Coahuila | Aguilar C.N.,Autonomous University of Coahuila | Rodriguez-Herrera R.,Autonomous University of Coahuila | Michelena G.,Instituto Cubano Of Investigaciones Of Los Derivados Of La Cana Of Azucar
Brazilian Journal of Microbiology | Year: 2014

Agro-industrial wastes have been used as substrate-support in solid state fermentation for enzyme production. Molasses and sugarcane bagasse are by-products of sugar industry and can be employed as substrates for invertase production. Invertase is an important enzyme for sweeteners development. In this study, a xerophilic fungus Aspergillus niger GH1 isolated of the Mexican semi-desert, previously reported as an invertase over-producer strain was used. Molasses from Mexico and Cuba were chemically analyzed (total and reducer sugars, nitrogen and phosphorous contents); the last one was selected based on chemical composition. Fermentations were performed using virgin and hydrolyzate bagasse (treatment with concentrated sulfuric acid). Results indicated that, the enzymatic yield (5231 U/L) is higher than those reported by other A. niger strains under solid state fermentation, using hydrolyzate bagasse. The acid hydrolysis promotes availability of fermentable sugars. In addition, maximum invertase activity was detected at 24 h using low substrate concentration, which may reduce production costs. This study presents an alternative method for invertase production using a xerophilic fungus isolated from Mexican semi-desert and inexpensive substrates (molasses and sugarcane bagasse). © 2014, Sociedade Brasileira de Microbiologia. All rights reserved.

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