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Sobreira F.M.,Instituto Capixaba Of Pesquisa Assistencia Tecnica E Extensao Rural Incaper | de Oliveira A.C.B.,Embrapa Cafe Empresa de Pesquisa Agropecuaria de Minas Gerais EPAMIG | Pereira A.A.,Embrapa Cafe Empresa de Pesquisa Agropecuaria de Minas Gerais EPAMIG | Sakyiama N.S.,Federal University of Vicosa
Australian Journal of Crop Science | Year: 2015

This work evaluated the Híbrido de Timor germplasm and the potential of its derived progenies as genetic resources to improve coffee quality, with focus on the specialty coffee market. Thirty eight Coffea arabica genotypes, comprising Híbrido de Timor genotypes, their progenies and traditional cultivars were studied. The study was conducted in a non-replicated design with intercalated checks where coffee cherry fruits were selectively handpicked and washed. Bean quality (shape and size) was assessed by a set of coffee test screens, while the sensory quality was measured using the attributes of the Specialty Coffee Association of America (SCAA) Cupping Protocols. Simple correlations and principal component analyses (PCA) were applied to analyze the data. There was high correlation for most of the sensory attributes, mainly between flavor and the final score (0.96). Regarding bean quality variables, the bean sieve sizes 19 and 18 correlated positively with the average sieve size (0.84 to 0.93) and negatively with the peaberry sieve (-0.55 to -0.69). The two first principal components illustrated genetic diversity for beans and cup quality. Some Híbrido de Timor and Catimor accessions, including UFV 454-43 and UFV 390-52, respectively, presented greater quality and may meet the interests of coffee breeders. For these, tasters described special nuances related to chocolaty, caramelly, fruity and flowery tastes. The Híbrido de Timor germplasm and its derivatives show potential to be used in coffee breeding programs which seek quality for the specialty coffee market.

de Almeida E.J.,Sao Paulo State University | Scaloppi E.M.T.,Sao Paulo State University | de Jesus N.,BASF | Benassi A.C.,Instituto Capixaba Of Pesquisa Assistencia Tecnica E Extensao Rural Incaper | And 2 more authors.
Ciencia e Agrotecnologia | Year: 2010

The Malay Apple [Syzygium malaccense (L.) Merr. & L.M. Perry] is an option for the producer as its fruits are attractive and well accepted by the consumer. The difficulty of culture of this species is that the plant is very tall and has a long juvenile period when propagated by seed, making its vegetative propagation necessary to anticipate the productive period and decrease its size. The possibility of vegetative propagation of this species was studied by cutting, layering, and grafting. In the cutting experiment, three doses of indolbutyric acid (IBA) (0, 1,000, 3,000, and 5,000 mg L-1) were tested in two lengths of herbaceous cuttings (15 and 25 cm). In the layering experiment, two periods of layering (summer and autumn) and four doses of IBA (0, 1,000, 4,000, 7,000, and 10,000 mg kg-1) were evaluated. For grafting, the compatibility between S. malaccense and S. jambos (L.) Alston as rootstock was studied with two diameters (0.5 and 0.8 cm) and in two periods (winter and summer), by method of full graft. For cuttings, the percentage of rooting was 20%, independently of the IBA doses, except for 5,000 mg L-1 that showed negative effect on 15 cm cuttings; layering and grafting were not successful in the studied conditions.

Sales E.F.,Instituto Capixaba Of Pesquisa Assistencia Tecnica E Extensao Rural Incaper | Sales E.F.,University of Cordoba, Spain | Ernesto Mendez V.,University of Vermont | Caporal F.R.,University of Pernambuco | Faria J.C.,University Estadual Of Santa Cruz
Agroecology and Sustainable Food Systems | Year: 2013

Coffee is a very important product in the State of Espírito Santo, Brazil, and most of it is planted as unshaded coffee monocultures, with few growers managing shaded coffee agroforestry systems (AFS). To analyze the opportunities and challenges associated with coffee agroforestry management, we conducted 58 semistructured interviews with coffee growers. In addition, we conducted a field investigation that tested production of Coffea canephora with the shade trees Australian Cedar (Toona ciliata), Jequitibá (Cariniana legalis), and Teak (Tectona grandis). Of the 58 interviewed farmers, 64% (37) were satisfied with the AFS. One of the main factors that caused satisfaction was obtaining income from sources other than coffee. Unsatisfied farmers mentioned the competition between shade trees and coffee shrubs. Cedar was the shade tree that grew most and reduced coffee production, while the combination with Jequitibá maintained more stable yields.We conclude that the higher the growth rate of trees, the higher the negative impact on the coffee production in the study areas. © Taylor & Francis Group, LLC.

Costa H.B.,Federal University of Espirito Santo | Fernandes P.M.B.,Federal University of Espirito Santo | Romao W.,Federal University of Espirito Santo | Romao W.,Instituto Federal Of Educacao | And 2 more authors.
Industrial Crops and Products | Year: 2014

Bioproducts separation and purification processes are an important segment of the biotechnical industry. Bromelain is an enzyme which has great commercial value and is of wide interest in the pharmaceutical, alimentary, and textile industries, among others. The goal of this study was to develop a new method for bromelain purification from the stem residues resulting from agricultural processing of the pineapple plant. Bromelain was purified using two liquid chromatography steps, ion exchange plus gel filtration chromatography. Use of the methodology which was developed produced an enzyme with a molecular weight of 30. kDa (confirmed by SDS-PAGE), high recovery of enzymatic activity (89%), and with a purification factor of 16.93, a result superior to the methodologies described in the literature. HPLC showed the presence of two peaks in the ion exchange chromatogram and only one protein in the gel filtration chromatogram. Results indicate that, depending on the destination of the bromelain, the process can be stopped after the first purification step. The MALDI-TOF MS provided the peptide mass fingerprint of bromelain and MALDI-MS/MS the fragmentation profile and sequencing of the ions of m/z 951 and 1584. Thus, the connectivity and chemical structure of bromelain was confirmed. Moreover, besides its superiority to other methodologies, it can be applied to take advantage of the agricultural and industrial pineapple plant residues. © 2014 Elsevier B.V.

Prezotti L.C.,Instituto Capixaba Of Pesquisa Assistencia Tecnica E Extensao Rural Incaper | Braganca S.M.,Instituto Capixaba Of Pesquisa Assistencia Tecnica E Extensao Rural Incaper
Coffee Science | Year: 2013

To expand the knowledge base concerning nutrition of conilon coffee, this work was developed to determine the dry mass and macro and micronutrient accumulation of different genetic sources of conilon coffee. The genetic sources used for the study were: EMCAPER 8151 (a cultivar propagated by seed) and clones 02, 03, 14, 23 and 120. During the frst fve years after planting, entire plants of each genetic material were collected monthly, divided into parts (leaves, branches, stem, root, and fruit), and the amount of dry matter (biomass) and the nutrient content were determined. On the basis of these data the nutrient content in each part of the plants as a function of age was estimated. The 02 and 23 clones produced the largest amount of dry mass of leaves, and clone 23 showed greater accumulation of dry mass of vegetative part. The largest fruit dry mass production was the clone 02, followed by clone 120. The ratios of the averages of the dry mass of leaf, twig, stem, root and fruit of genetic material were 19%, 12%, 42%, 15% and 12%, respectively. Contents of N, P and K in all parts of the plants declines over the months after flowering. Clone 23 showed the highest rate of accumulation of N (0.09298 g1 plant1 day1) and clone 14 presented the lower rate of accumulation (0.04696 g plant1 day1). Clone 02 showed the highest rate of accumulation of P (0.01483 g plant1 day1) and the EMCAPER 8151 showed the highest rate of accumulation of K (0.04919 g plant1 day1). The accumulated nutrients content in various parts of plants genetic material is atributed mainly to differences in dry mass production than to differences in nutrient levels.

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