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The Chlamydophila abortus was previously known as Chlamydia psittaci sorovar 1, it is a Gram negative and obligate intracellular bacteria. This microorganism is frequently related with reproductive manifestation in ovines, goats and bovines. The major manifestation are Enzootic abortion in bovines and enzootic abortion in small ruminants. There are few literary material about clamidofilosis in Brazil, so the present research had the objective to determine the presence of complement fixing antibodies anti-Chlamydophila abortus, correlating the results with the clinical examination and historical of the animals, besides variation in the zootecnic index, especially in the reproductive characters like high index of cio repetition, high abortion number, high neonatal mortality number etc. It was tested for the complement fixation test 220 serum ovine samples, from 26 properties, distributed in 19 cities, with historical of reproductive manifestation. It was found 19.55% (43/220) of positive tests to Chlamydophila abortus, obtaining 61.53% of focus occurrence. In the study low antibodies titers were found, not superior of 64. Abortion was the major reproductive manifestation observed, expressing 65.12% (28/43) of the total number animals serum positive, followed by estrus repetition and weak lamb born, with a frequency of 6.98% (3/43) and, lastly, neonatal mortality death with 4.65% (2/43). No significant association was observed between serum positivity and these factors.

Rodrigues M.M.,Federal University of Grande Dourados | Uchoa M.A.,Federal University of Grande Dourados | Ide S.,Instituto Biologico Of Sao Paulo
Brazilian Journal of Biology | Year: 2013

Dung beetles (Coleoptera: Scarabaeoidea) in three landscapes in Mato Grosso do Sul, Brazil. Dung Beetles are important for biological control of intestinal worms and dipterans of economic importance to cattle, because they feed and breed in dung, killing parasites inside it. They are also very useful as bioindicators of species diversity in agricultural or natural environments. The aims of this paper were to study the species richness, and abundance of dung beetles, helping to answer the question: are there differences in the patterns of dung beetle diversity in three environments (pasture, agriculture and forest) in the municipality of Dourados, in the state of Mato Grosso do Sul. A total of 105 samplings were carried out weekly, from November 2005 to November 2007, using three pitfall traps in each environment. The traps were baited with fresh bovine dung, and 44,355 adult dung beetles from 54 species were captured: two from Hyborosidae and 52 from Scarabaeidae. Five species were constant, very abundant and dominant on the pasture, two in the agricultural environment, and two in the environment of Semideciduous forest. Most of the species were characterised as accessories, common and not-dominant. The species with higher abundance was Ataenius platensis Blanchard, 1844. The indexes of Shannon-Wiener diversity were: 2.90 in the pasture, 2.84 in the agricultural environment and 2.66 in the area of native forest. The medium positive presence of dung beetles in the traps in each environment were: 36.88, 42.73 and 20.18 individuals per trap, in the pasture, agricultural environment and in the native forest, respectively. The pasture environment presented a higher diversity index. The species diversity of dung beetles was superior where there was higher abundance and regularity of resource (bovine dung).

Dos Santos A.F.,Brazilian Agriculture Research Corporation Embrapa Florestas | Tessmann D.J.,State University of Maringa | Alves T.C.A.,State University of Maringa | Vida J.B.,State University of Maringa | Harakava R.,Instituto Biologico Of Sao Paulo
Journal of Phytopathology | Year: 2011

In an area reforested with Brazilian pine (Araucaria angustifolia) located in Paraná State, southern Brazil, 20- to 40-year-old trees representing 0.2% of the surveyed area had symptoms of root and crown rot, yellowing and browning of leaves from the uppermost branches and death. Three Phytophthora isolates obtained from diseased plant tissue were tested against 1-year-old Brazilian pine seedlings and found to display positive pathogenicity. Based on their morphological and physiological characteristics, the isolates were identified as Phytophthora cinnamomi. A GenBank BLAST search of partial sequences from the β-tubulin and elongation factor-1α genes, as well as the ITS regions and 5.8S gene of rDNA, confirmed the species identification. This is the first report of the involvement of this pathogen on the aetiology of Brazilian pine root and crown rot. © 2010 Blackwell Verlag GmbH.

Correa M.F.,University of Sao Paulo | Pinto A.P.C.,University of Sao Paulo | Rezende J.A.M.,University of Sao Paulo | Harakava R.,Instituto Biologico Of Sao Paulo | Mendes B.M.J.,University of Sao Paulo
European Journal of Plant Pathology | Year: 2015

Passion fruit woodiness disease, which is caused by the potyvirus Cowpea aphid borne mosaic virus (CABMV), is the primary constraint for passion fruit production in Brazil. Transgenic Passiflora alata lines that contain a CABMV-derived coat protein gene fragment in a hairpin configuration were obtained via Agrobacterium tumefaciens-mediated transformation. The plants were propagated and the reaction to CABMV infection was evaluated after three mechanical and one viruliferous vector inoculations. After three mechanical inoculations, two lines from a total of 21 transgenic lines tested maintained all four propagated clones symptomless. After the fourth inoculation, all transgenic lines presented at least one propagated clone infected with CABMV. However, 20 propagated clones from different transgenic lines remained asymptomatic. These asymptomatic plants were analyzed by RT-PCR and CABMV was detected in 17 plants. The estimated viral titers in these plants, which were determined by RT-qPCR, were consistently low compared with those of the positive control (non-transgenic inoculated plants). A biological virus recovery test was performed using leaf extracts from the three RT-PCR negative propagated clones and the absence of the CABMV was confirmed. The results of the present study indicate that the incorporation of CABMV-gene fragments into the Passiflora genome may influence the resistance of these plants to the pathogen. © 2015, Koninklijke Nederlandse Planteziektenkundige Vereniging.

Araujo C.P.,Federal University of Mato Grosso | Osorio A.L.A.R.,Federal University of Mato Grosso | Jorge K.S.G.,Federal University of Mato Grosso | Ramos C.A.N.,Brazilian National Council for Scientific and Technological Development | And 11 more authors.
PLoS ONE | Year: 2014

In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. © 2014 Araújo et al.

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