Instituto Biologico IB

São Paulo, Brazil

Instituto Biologico IB

São Paulo, Brazil
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Fiorini L.C.,Instituto Biologico IB | Craveiro A.B.,Federal University of Amazonas | Mendes M.C.,Instituto Biologico IB | Neto L.C.,Refugio da Vida Silvestre Sauim Castanheiras | Da Silveira R.,Federal University of Amazonas
Revista Brasileira de Parasitologia Veterinaria | Year: 2014

The Boa constrictor is one of the world’s largest vertebrate carnivores and is often found in urban areas in the city of Manaus, Brazil. The morphological identification of ticks collected from 27 snakes indicated the occurrence of Amblyomma dissimile Koch 1844 on all individuals sampled. In contrast, Amblyomma rotundatum Koch was found on only two snakes. An analysis of the 16S rRNA molecular marker confirmed the morphological identification of these ectoparasites. © 2014, Brazilain Coll Veterinary Parasitology. All rights reserved.


Torres L.C.,Catholic University Dom Bosco | Lourencao A.L.,Instituto Agronomico IAC | Costa V.A.,Instituto Biologico IB | Souza B.,Federal University of Lavras | And 2 more authors.
Neotropical Entomology | Year: 2014

Collections of natural enemies of Bemisia tabaci biotype B (Genn.) (Hemiptera: Aleyrodidae) were made in Lavras, state of Minas Gerais, Brazil. In the greenhouse, 6,495 predators and 16,628 parasitoids belonging to three families were collected. In the field, 267 predators and 344 parasitoids belonging to five families were found. For the first time in Brazil, five species of predators associated with this whitefly were reported. Because of the diversity of natural enemies of B. tabaci biotype B recorded, this study points out the importance of these data for studies on integrated pest management. © 2013 Sociedade Entomológica do Brasil.


PubMed | Refugio da Vida Silvestre Sauim Castanheiras, Federal University of Amazonas and Instituto Biologico IB
Type: Journal Article | Journal: Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria | Year: 2014

The Boa constrictor is one of the worlds largest vertebrate carnivores and is often found in urban areas in the city of Manaus, Brazil. The morphological identification of ticks collected from 27 snakes indicated the occurrence of Amblyomma dissimile Koch 1844 on all individuals sampled. In contrast, Amblyomma rotundatum Koch was found on only two snakes. An analysis of the 16S rRNA molecular marker confirmed the morphological identification of these ectoparasites.


PubMed | Instituto Agronomico IAC, EMBRAPA - Empresa Brasileira de Pesquisa Agropecuária, Catholic University Dom Bosco, Federal University of Lavras and Instituto Biologico IB
Type: Journal Article | Journal: Neotropical entomology | Year: 2016

Collections of natural enemies of Bemisia tabaci biotype B (Genn.) (Hemiptera: Aleyrodidae) were made in Lavras, state of Minas Gerais, Brazil. In the greenhouse, 6,495 predators and 16,628 parasitoids belonging to three families were collected. In the field, 267 predators and 344 parasitoids belonging to five families were found. For the first time in Brazil, five species of predators associated with this whitefly were reported. Because of the diversity of natural enemies of B. tabaci biotype B recorded, this study points out the importance of these data for studies on integrated pest management.


Duarte L.M.L.,Instituto Biologico IB | Alexandre M.A.V.,Instituto Biologico IB | Gobatto D.,Instituto Biologico IB | Kitajima E.W.,University of Sao Paulo | Harakava R.,Laboratory Of Bioquimica
Plant Disease | Year: 2014

In November 2012, plants of Russell prairie gentian (Eustoma grandiflorum, Lisianthus russellianus) were collected from a commercial greenhouse in Atibaia, SP, Brazil, displaying necrotic spots on leaves and necrosis on stems, followed by generalized systemic necrosis. Disease symptom incidence was estimated at 10%. Preliminary electron microscopy observations of negatively stained leaf extracts prepared from those lesions revealed the presence of a large number of spherical tospovirus-like, approximately 100 nm in diameter. Samples of infected leaves were ground in 0.01 M phosphate buffer containing 0.5% sodium sulphide and mechanically inoculated in six plants of each species of Nicotiana glutinosa, N. tabacum cv. White Burley, N. megalosiphon, N. debneyii, Datura stramonium, Chenopodium amaranticolor, C. quinoa, and E. grandiflorum. All inoculated plants displayed local lesions 4 to 5 days after inoculation, while N. debneyii and D. stramonium showed systemic symptoms, typical of Tospovirus infection. In addition, E. grandiflorum reproduced the original symptoms. Total RNA was extracted from infected E. grandiflorum and D. stramonium, and reverse transcription (RT)-PCR was performed using universal primers BR60 and BR65 (2) targeting conserved regions of the nucleocapsid gene (N). The amplification products of approximately 450 bp were purified, cloned, and sequenced. The unknown virus was identified as Chrysanthemum stem necrosis virus (CSNV-Lis) based on host range and nucleotide sequence (Genbank Accession No. KC894721) and showed 99% identity with a CSNV chrysanthemum isolate from Japan (AB600872). Maximum likelihood phylogenetic analysis using nine homologous CSNV sequences available in GenBank classified CSNV-Lis into a monophyletic group formed by chrysanthemum isolates from Japan and China while a Japanese lisianthus isolate was separately clustered. CSNV is a member of the genus Tospovirus (Bunyaviridae) and was first reported on chrysanthemum in Brazil (1) and later in the Netherlands, Slovenia, United Kingdom, and Japan (3). Despite scattered recent reports of CSNV, the simultaneous production of chrysanthemum and lisianthus crops along the year by Brazilian farmers has contributed to the virus maintenance in the field. The high identity between Brazilian and Japanese isolates of CSNV suggest a possible reintroduction of the virus through exchange of vegetative propagating material.


Rivas E.B.,Instituto Biologico IB | Bodi E.C.A.,Rua Esteves Junior | Harakava R.,University of Sao Paulo | Gregori F.,University of Sao Paulo | Goncalves M.C.,University of Sao Paulo
Pesquisa Agropecuaria Brasileira | Year: 2016

The objective of this work was to describe the occurrence of quarantine Tulip breaking virus (TBV, synonym Lily mottle virus - LMoV) and Lily symptomless virus (LSV), and their respective molecular analyses, to provide data for supporting TBV removal from the Brazilian A1 quarantine pest list, since this virus has spread among the main commercial lily crops in Brazil. The occurrence of these viruses was detected in 12 cultivation areas through multiplex reverse transcription (RT-PCR), using specific primers to genes encoding the respective coat proteins (CP). Eight fragments of 800 nucleotides (nt) obtained from the LMoV-infected lilies and nine fragments of 600 nt from LSV-infected lilies were sequenced. Phylogenetic tree reconstruction showed a robust branch containing the LMoV Brazilian sequences, other LMoV isolates, TBV, and Tulip band breaking virus, suggesting that all are LMoV isolates, although they are clustered into two subgroups. Phylogenetic analysis also showed a robust branch supporting all Brazilian and other LSV sequences, except for an LSV Japanese isolate. Recombination analyses also showed an LMoV recombinant isolate, whereas no recombination events were found among LSV isolates. Lily mottle virus is the prevalent virus in lily crops in Brazil, in single and mixed infections with LSV or Cucumber mosaic virus (CMV).


Bernardes F.S.,University of Sao Paulo | Patricio F.R.A.,Instituto Agronomico IAC | Santos A.S.,Instituto Biologico IB | dos Freitas S.S.,Embrapa Tabuleiros Costeiros
Summa Phytopathologica | Year: 2010

Rhizobacteria of the fluorescent group of Pseudomonas spp. Were selected for both their capacity in the growth promotion of lettuce seedlings (Lactuca sativa L.) and antagonistic activity against Pythium aphanidermatum from test in vitro. Then, in hydroponic system was assayed the previous application of the Pseudomonas strains in order to reduce damages caused by P. aphanidermatum in cucumber (Cucumis sativus) for induced systemic resistance (ISR), using a technical splitroot. The variables shoots and roots dry weight and root length were used in evaluation. The production of antagonistic and/or growth promoters compounds by rhizobacteria strains was observed to associate its activity with the results obtained in the tests under hydroponic system and in vitro conditions. In the in vitro experiment, strains Ps 140B and Ps 140C provided the largest development of plants (root and hypocotyls) in the treatments with and without pathogen. Some strains tested indicating a possible expression of ISR in in vivo experiments. No correspondence among the compounds produced by rhizobacteria, plant growth promotion and bioprotection was detected.


Batista I.C.,Instituto Biologico IB | Eiras M.,Instituto Biologico IB | Harakava R.,Instituto Biologico IB | Colariccio A.,Instituto Biologico IB
Bragantia | Year: 2012

A Tymovirus isolated from seeds of tomato (Solanum lycopersicum) cv. 'Paronset' with necrosis, named TyToRS08 isolate, was characterized by its host range, physical-chemical, serological and molecular properties. Firstly, it was successfully transmitted by mechanical inoculation by infected seeds, and the host range was restricted to species of Amaranthaceae, Chenopodiaceae and Solanaceae. The symptoms induced by the virus in tomatoes 'Alambra', 'Thomas', 'Diva', 'Romana' and 'Graziela' were mainly mosaic and white necrosis. Nicotiana sylvestris, N. tabacum 'Samsun', 'White Burley' and 'Xanthi' were symptomless. The virus was detected in seeds from five tomato cultivars, but it was not detected in seedlings from these infected seeds. The purified preparation had a high concentration of 1.04 mg mL-1, and the title of the polyclonal antiserum produced, evaluated in PTA-ELISA, was 1/32.768. RT-PCR was performed with degenerated primers directed to the ORF 1 of the tymoviruses, resulting in a DNA fragment of 700 bp. The sequence analysis revealed that TyToRS08 isolate presents higher values of percentage of identity with other species of Tymovirus. The highest identity values were under 70%, indicating that TyToRS08 should be a distinct species of the genus Tymovirus, with great epidemiological importance due to its high stability and potential to be spread by tomato seeds.


De Souza A.D.,Instituto Biologico IB | De Souza A.D.,Laboratorio Of Fitossanidade | Aoyama E.M.,Centro Universitario Norte do Espirito Santo | Aoyama E.M.,Federal University of Espirito Santo | Furlan M.R.,São Paulo State University
Revista em Agronegocio e Meio Ambiente | Year: 2015

Passiflora ligularis Juss. is highly consumed by people, especially in natura, as juice and ice-cream. However, no successful research is extant on its development and adaptation in Brazil. The high costs of granadilla and the difficulty to buy it, even in supermarkets, justify analysis to verify the adaptability of the species, with production potential, at least in some regions of the country. Current assay is a contribution for research on the behavior of granadilla in Brazil so that information on the effect of time and storage conditions may be obtained for seed germination and the early development stages of the seedlings. Granadilla seeds came from Colombian fruit and the assay focused on the evaluation of seed quality with three different storage periods, with emergence velocity index (EVI) and percentage of seedlings' germination and development. Treatments comprised Treatment A - seeds stored for 24 months; Treatment B - seeds stored for 3 months; Treatment C - seeds without storage. Randomized design with four replications of 50 seeds was employed and comparisons made by Tukey's test at 1% probability. Seedlings should be obtained from seeds kept in cold chambers for a period of more than 101 days. Seeds which had been only recently removed from the fruit have low germination percentage and small height growth when compared with plants from seeds stored for 24 months. © 2015, University Center of Maringa. All rights reserved.


PubMed | Instituto Agronomico Of Campinas Iac, Agencia Paulista de Tecnologia dos Agronegocios APTA and Instituto Biologico IB
Type: | Journal: SpringerPlus | Year: 2015

Passion fruit woodiness may be caused by Cowpea aphid-borne mosaic virus (CABMV) and is currently the major passion fruit disease in Brazil. To assess the virus-vector-host interactions, a newly introduced golden passion fruit plantation located in eastern region of So Paulo State, Brazil, was monitored.Dissemination of CABMV was determined analyzing golden passion fruit plants monthly for 18months by PTA-ELISA. Seasonality and aphid fauna diversity was determined by identification of the captured species using yellow sticky, yellow water-pan and green tile traps. Population composition of the aphid species was determined using the descriptive index of occurrence, dominance and general classification and overlap of species in the R program.Analyses of species grouping afforded to recognize 14 aphid species. The genus Aphis represented 55.42% of the species captured. Aphid species formed two distinct clusters, one of which was characterized by the diversity of polyphagous species that presented high potential to spread CABMV.The low abundance and diversity of aphid species did not interfere negatively in the CABMV epidemiology. The genus Aphis, particularly Aphis fabae/solanella and A. gossypii, was crucial in the spread of CABMV in passion fruit orchards in the eastern State of So Paulo.

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