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Hasegawa M.Y.,University of Sao Paulo | Custodio de Souza Hunold Lara M.D.C.,Instituto Biologico APTA SAA | Monteforte Cassaro Villa Lobos E.,Instituto Biologico APTA SAA | Carrillo Gaeta N.,University of Sao Paulo | And 4 more authors.
Small Ruminant Research | Year: 2017

Caprine Arthritis Encephalitis is a cosmopolitan disease that affects small ruminant production and causes economic losses. Although the virus has been identified in different parts of the female reproductive tract, including the uterus, there is a lack of studies that discussed its vertical transmission. The aim of this study was to evaluate the transmission of caprine arthritis encephalitis virus (CAEV) from naturally infected females to their offspring. Five known positive goats for CAEV were artificially inseminated with fresh semen obtained from a known negative male for CAEV. After parturition, twelve kids were followed for 12 months, and the vertical transmission of CAEV was studied by applying agar gel immunodiffusion test (AGID), competitive enzyme linked immunosorbent assay (cELISA) and nested-polimerase chain reaction (nested-PCR). From a total of 12 kids, six were positive in nested-PCR. None of them was positive in cELISA and AGID. This study demonstrated the vertical transmission of caprine arthritis encephalitis virus to the kids. The presence of infected females must be avoided due to the rapid spread of the virus and the economic losses associated with the infection. © 2017

Hasegawa M.Y.,University of Sao Paulo | Meira E.B.S.,University of Sao Paulo | Lara M.C.C.S.H.,Instituto Biologico APTA SAA | Castro R.S.,University of Pernambuco | And 10 more authors.
Small Ruminant Research | Year: 2016

Caprine arthritis encephalitis (CAE) is a multisystem infectious disease caused by a small ruminant lentivirus that is widely disseminated in the goat flock of southeastern Brazil. Little information about its variants is available in Brazil. Phylogenetic analysis was performed on the sequences of 14 clinical isolates of CAEV that obtained from mammary glands, lung, brain, milk and blood of goats with any clinical form of the disease in Sao Paulo State, Brazil. Part of the pol gene of CAEV was amplified by nested-PCR and sequenced. These sequences were compared with 42 reference strains previously described and commonly used in phylogenies. The sequences derived from this study were more related to caprine strains than to ovine strains. Additionally, subtype B of the CAEV group was detected in different clinical samples with distinct clinical symptoms. Subgroup B1 was the predominant genotype. A subgroup C-like was isolated from animals that presented interstitial indurative mastitis. Animals from which the sequences belonged to this subgroup presented interstitial mastitis induration, chronic arthritis and chronic interstitial pneumonia. Subgroup C sequences have not been reported in Brazil previously, while circulating subgroup B1 variants were already known to be present in the country. This study provides new information about CAEV variants in Brazil, since we isolated and reported the subgroup C-like. A variant from B1 subgroup was the predominant genotype. More studies are necessary in order to confirm the presence of CAEV genotype C or even a new genotype. CAEV is an important disease of goat production and all knowledge is necessary to understand its epidemiology and, prevent this illness. © 2016 Elsevier B.V.

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