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do Nascimento E.S.P.,Federal University of São Carlos | de Oliveira C.R.,Federal University of São Carlos | de Souza Goncalves P.,Instituto Agronomico Campinas IAC | da Costa R.B.,Federal University of Mato Grosso | And 3 more authors.
Crop Breeding and Applied Biotechnology | Year: 2011

In this study, the effect of rootstock on grafting through metabolomic analysis of latex (Hevea brasiliensis) samples was verified by 1 H nuclear magnetic resonance (NMR) and multivariate data analysis. Sixteen metabolites present in the latex cytosol were characterized by NMR. PCA analysis showed that the latex samples of the RR and GR groups can be differentiated. The GR group samples present a metabolic profile similar to the RR group samples, while the RG group is in an intermediate position between RR and GG groups. Sucrose and formate contributed greatly to the separation obtained by PCA, presenting a good correlation between the results. 1H NMR was an efficient technique to differentiate latex samples from different types of rootstocks and grafting and in the future could be used to predict rubber production by latex analysis.

Pinto-Maglio C.A.F.,Instituto Agronomico Campinas IAC | Pommer C.V.,State University of Norte Fluminense | Pierozzi N.I.,Instituto Agronomico Campinas IAC
Caryologia | Year: 2010

Giemsa staining technique and fl uorescent chromosome banding with CMA3 and DAPI were applied to the mitotic chromosomes of Vitis champinii, V. cinerea, V. girdiana, V. labrusca, V. rotundifolia, V. rupestris and V. vinifera for the purpose of chromosome measurement and constitutive heterochromatin characterization at the cytochemical level, respectively. Both fl uorescent CMA and conventional Giemsa staining constituted a valuable tool for chromosome characterization. Karyomorphometric data obtained after Giemsa staining allowed for an average ideogram and karyotype formulae based on chromosome length for the species. V. champinii and V. girdiana distinguished from the other five species by means of the total haploid chromosome length, by the longest chromosome length and by the average chromosome length. The seven grape species have moderate chromosome asymmetry values and were classified on 2A Stebbins' category. Positive CMA bands were seen in all species. V. girdiana distinguished from the other species solely by the presence of two CMA+ bands, while V. champinii, V. cinerea, V. labrusca, V. rotundifolia and V. rupestris had four bands. V. vinifera showed chromosome heteromorphism for CMA bands. No clearly visible DAPI+ band was seen in the species. According to the present observations, it seems that the evolutionary process of speciation involving North American and European Euvitis species studied, resulted in some discrete changes in chromosome measurements and also in heterochromatin base composition of at least one species. These data enlarge the chromosomal information of the genus Vitis and make possible further comparative studies into the Vitaceae family.

Pierozzi N.I.,Instituto Agronomico Campinas IAC | Baroni R.M.,University of Campinas
Cytologia | Year: 2014

Acetic orcein as well as C- and NOR-banding (silver staining) techniques were applied to the mitotic chromosomes of ramie Boehmeria nivea var. nivea (L.) Gaudisch cv. Miyasaki for chromosome characterization. C-banding was also applied in pachytene and diakinesis meiotic phases for heterochromatin localization. The species showed a predominance of submetacentric chromosomes as observed in karyotype formula: 1m + 12sm + 1sms , as well as a moderate karyotype asymmetry as revealed by the Stebbins criteria and Romero-Zarco and Paszko indices. C-bands were preferentially localized in the chromosome short arms of the mitotic chromosomes. Large blocks of pericentromeric heterochromatin C-bands were seen at pachytene meiotic phase, while at diakinesis phase, C-bands were located on the chromosome ends as well as nearby or on the chiasmata. The interphase nuclei of mitotic cells were classified as prochromosome type, which is the same as S2 type. One pair of chromosomes with satellite segment was seen in the genotype studied after acetic orcein and silver staining (NOR-band). Chromosome morphometric data along with NOR localization were suitable to characterize the Miyasaki cultivar.

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