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Yoshioka C.R.M.,University of Sao Paulo | Martinez M.B.,University of Sao Paulo | Brandileone M.C.C.,Federal University of Sao Paulo | Brandileone M.C.C.,Instituto Adolfo Lutz IAL | And 5 more authors.
Jornal de Pediatria | Year: 2011

Objectives: To identify the most common pneumococcal serotypes in children hospitalized with invasive pneumonia, correlate isolated serotypes with those included in conjugate vaccines, and ascertain the sensitivity of the isolated pneumococcal strains to penicillin and other antibiotics. Methods: From January 2003 to October 2008, a retrospective study of hospitalized children with a diagnosis of Streptococcus pneumoniae pneumonia was conducted at the university hospital of Universidade de São Paulo. Criteria for inclusion were: age greater than 29 days and less than 15 years, radiological and clinical diagnosis of pneumonia, and isolation of Streptococcus pneumoniae in blood cultures and/or pleural effusion. Results: The study included 107 children. The most common serotypes were 14 (36.5%), 1 (16%), 5 (14.6%), 6B (6.3%) and 3 (4.2%). The proportion of identified serotypes contained in the heptavalent, 10-valent and 13-valent conjugate vaccines was 53.1, 86.5, and 96.9%, respectively. Pneumococcal strains were sensitive to penicillin (minimum inhibitory concentration, MIC ≤ 2 ?g/mL) in 100 cases (93.5%) and displayed intermediate resistance (MIC = 4 μg/mL) in 7 cases (6.5%). No strains were penicillin-resistant (MIC ≥ 8 μg/mL) according to the Clinical and Laboratory Standards Institute 2008 standards. Tested isolates were highly sensitive to vancomycin, rifampicin, ceftriaxone, clindamycin, erythromycin, and chloramphenicol. Conclusions: Our results confirm a significant potential impact of conjugate vaccines, mainly 10-valent and 13-valent, on invasive pneumonia. Furthermore, susceptibility testing results show that penicillin is still the treatment of choice for invasive pneumonia in our setting. Copyright © 2011 by Sociedade Brasileira de Pediatria.

Santos S.V.,University of Sao Paulo | Strefezzi R.F.,University of Sao Paulo | Kanamura C.T.,Instituto Adolfo Lutz IAL | Takakura C.F.H.,University of Sao Paulo | And 2 more authors.
Journal of Medical Primatology | Year: 2014

Background: Toxoplasmosis led to the death of two Brachyteles arachnoides, an endangered atelid. Methods: The diagnosis was established by necropsy, histopathological, immunohistochemical and ultrastructural changes. Results: The analysis confirms the presence of Toxoplasma gondii. Conclusions: This report contributes to the development of protocols for health surveillance on maintenance and conservation of southern muriquis. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Santos S.,Northeast Brazil College | de Arauz L.J.,Instituto Adolfo Lutz IAL | Baruque-Ramos J.,University of Sao Paulo | Lebrun I.,Instituto Butantan IB | And 3 more authors.
Vaccine | Year: 2012

Serogroup B outer membrane vesicles (OMV) with iron regulated proteins (IRP) from Neisseria meningitidis constitute the antigen for the vaccine against the disease caused by this bacterium. Aiming to enhance final OMV concentration, seven batch experiments were carried out under four different conditions: (i) with original Catlin medium; (ii) with original Catlin medium and lactate and amino acids pulse at the 6th cultivation hour; (iii) with Catlin medium with double initial concentrations of lactate and amino acids and (iv) Catlin medium without glycerol and with double initial concentrations of lactate and amino acids. The cultivation experiments were carried out in a 7-L bioreactor under the following conditions: 36°C, 0.5atm, overlay air 1L/min, agitation: 250-850rpm, and O2 control at 10%, 20h. After lactate and amino acids exhaustion, cell growth reached stationary phase and a significant release increase of OMV was observed. According to the Luedeking & Piret model, OMV liberation is non-growth associated. Glycerol was not consumed during cultivation. The maximum OMV concentration value attained was 162mg/L with correspondent productivity of 8.1mg/(Lh) employing Catlin medium with double initial concentrations of lactate and amino acids. The obtained OMV satisfied constitution and protein pattern criteria and were suitable for vaccine production. © 2012 Elsevier Ltd.

Santos S.V.,University of Sao Paulo | Strefezzi R.F.,University of Sao Paulo | Takakura C.F.H.,University of Sao Paulo | Kanamura C.,Instituto Adolfo Lutz IAL | And 2 more authors.
Journal of Medical Primatology | Year: 2012

Background : An adult male Brachyteles arachanoides, kept in captivity since 1990, was found dead without apparent clinical evidence. Methods : Necropsy report, histopathology, immunohistochemistry, and ultrastructural examination were conducted. Results : Pulmonary syncytial cells were positive for respiratory syncytial virus (RSV), and ultrastructural examination revealed viral particles inside macrophages compatible with the Paramyxoviridae family. Conclusions: Muriquis are susceptible to RSV pneumonia followed by respiratory distress syndrome and death. © 2012 John Wiley & Sons A/S.

Castejon M.J.,Instituto Adolfo Lutz IAL | Yamashiro R.,Instituto Adolfo Lutz IAL | Oliveira C.A.F.,Instituto Adolfo Lutz IAL
Jornal Brasileiro de Patologia e Medicina Laboratorial | Year: 2015

Introduction: Blood samples collected on filter paper (dried blood spot [DBS]) is an immunoassay that has been used for antibodies screening. Objective: To evaluate the strategy of DBS blood collection for detection of HIV antibodies, evaluation of Q-Preven HIV 1 + 2 - DBS kit lot, and to analyze the stability of DBS samples. Method: Blood collection on DBS was performed according to World Health Organization (WHO) recommendations. The evaluation of the kit lot for HIV antibodies detection was performed using delta (d) values from the results of 774 DBS samples from volunteers men who have sex with men (MSM) recruited in the central region of São Paulo city, Brazil. Result: DBS blood collection was performed without complications. The positive (5.26) and negative (5.23) delta values allowed to clearly differentiate HIV antibodies reactive and non-reactive samples. We observed good performance of the kit lot and samples were stable on DBS form. Conclusion: Blood collection on DBS is feasible for the study of MSM population and is suitable for laboratory routine. The overall performance of Q Preven HIV-1 + 2 - DBS kit was satisfactory, having reached the quality levels required for the development of this study.

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