Institutional Biotech Hub

Imphal, India

Institutional Biotech Hub

Imphal, India
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Bag G.C.,Imphal College | Grihanjali Devi P.,Imphal College | Bhaigyabati T.,Institutional Biotech Hub
International Journal of Pharmaceutical Sciences Review and Research | Year: 2016

Aqueous and methanol extracts of Meyna laxiflora leaf, fruit pulp and seed were screened for the presence of phytochemical constituents. Total phenolic content, total flavonoid content and antioxidant activity in the extracts were also determined. Phytochemicals were extracted using soxhlet extraction method. Phytochemical screening showed the presence of most of the phytoconstituents in aqueous and methanol extracts of various parts of M. laxiflora. Methanol was found to be a better solvent for extraction of phytochemicals from M. laxiflora. Total phenolic content is expressed in terms of gallic acid equivalent (GAE) and was found to be highest in methanolic fruit pulp extract (129.54 ± 0.25mg /g of extract). Total flavonoid content is expressed in terms of quercetin equivalent (QE) and methanolic leaf extract recorded the highest (80.45 μg ± 0.16 /100g of dried extract) followed by fruit pulp and seed extracts. Results indicate that aqueous and methanol extracts of various parts of M. laxiflora were found to possess DPPH scavenging activity, ferric reducing power and total antioxidant activity in dose dependent manner. In the entire antioxidant assay performed methanol extracts were found to have higher activity than aqueous extracts and among the various parts of M. laxiflora used for the study, fruit pulp was showing higher activity than leaf and seed which may be due to higher total phenolic content. © 2016, Global Research Online. All rights reserved.


Lemino Singh K.,Institutional Biotech Hub | Bag G.C.,Imphal College
International Journal of PharmTech Research | Year: 2013

Hedychium is a rhizomatous plant belonging to family Zingiberaceae. In this present study, three different species of genus Hedychium i.e. H. spicatum, H. coronarium and H. rubrum were analysed quantitatively for the presence of major phytochemical compounds. The total phenolic contents in extracts of the three different species were also analyzed. The dried and powdered rhizomes of each species were extracted with distilled water using Soxhlet extraction method. Phytochemical screening of the water extract of H. spicatum showed the presence of phenolic compounds, flavonoids, reducing sugar (carbohydrate), protein, steroids and triterpenoids, cardiac glycosides, tannin, saponin and oil. Phytochemical screening of the water extracts of both H. coronarium and H. rubrum also showed the presence of similar groups of compounds. H. spicatum, H. coronarium and H. rubrum tested negative for the presence of alkaloids. Both H. spicatum and H. coronarium showed negative test for phlobatannin but H.rubrum indicated the presence of phlobatannin. The total phenolic contents of the water extracts of each species were estimated by using UV Visible Spectrophotometer and expressed as gallic acid equivalents (GAE). The total phenolic contents of the water extracts of H. spicatum, H. coronarium and H. rubrum in terms of gallic acid equivalent were 29.39 ± 0.01, 34.93 ± 0.01 and 66.48 ± 0.01 mg /g of extract powder respectively.


Lemino Singh K.,Institutional Biotech Hub | Ranjit Singh L.,Imphal College | Grihanjali Devi P.,Imphal College | Romabati Devi N.,Imphal College | And 2 more authors.
International Journal of PharmTech Research | Year: 2013

Hedychium is a rhizomatous perennial plant which belongs to family Zingiberaceae with various ethnomedicinal and ornamental significance. In this study, three different species of genus Hedychium i.e. H. spicatum, H. coronarium and H. rubrum were screened for the presence of major phytochemical compounds. The total phenolic contents of the three different species were also analyzed. The dried and powdered rhizomes of each species were extracted with methanol using Soxhlet extraction method. Phytochemical screening of methanolic extracts of each species was performed qualitatively. Phytochemical screening of the methanolic extract of H. spicatum showed the presence of phenolic compounds, flavonoids, alkaloids, reducing sugar (carbohydrate), protein, steroids and triterpenoids, cardiac glycosides, diterpene, tannin, saponin and oil. Phytochemical screening of the methanolic extracts of both H. coronarium and H. rubrum also showed the presence of similar groups of compounds but tested negative for the presence of alkaloids. Both H. spicatum and H. rubrum showed negative test for phlobatannin but H. coronarium indicated the presence of phlobatannin. The total phenolic contents of the methanolic extracts of each species were estimated by using UV Visible Spectrophotometer and expressed as gallic acid equivalents (GAE). The total phenolic contents of the methanolic extracts of H. spicatum, H. coronarium and H. rubrum in terms of gallic acid equivalent were 19.48±0.82, 26.22±1.17 and 28.16±0.45 mg /g of extract powder respectively.


Singh H.R.,Tocklai Tea Research Institute | Singh H.R.,Institutional Biotech Hub | Deka M.,Gauhati University | Das S.,Tocklai Tea Research Institute | Das S.,Institute of Bioresources and Sustainable Development
Functional and Integrative Genomics | Year: 2015

Tea is the second most consumed beverage in the world. A crop loss of up to 43 % has been reported due to blister blight disease of tea caused by a fungus, Exobasidium vexans. Thus, it directly affects the tea industry qualitatively and quantitatively. Solanum tuberosum class I chitinase gene (AF153195) is a plant pathogenesis-related gene. It was introduced into tea genome via Agrobacterium-mediated transformation with hygromycin phosphotransferase (hpt) gene conferring hygromycin resistance as plant selectable marker. A total of 41 hygromycin resistant plantlets were obtained, and PCR analysis established 12 plantlets confirming about the stable integration of transgene in the plant genome. Real-time PCR detected transgene expression in four transgenic plantlets (T28, C57, C9, and T31). Resistance to biotrophic fungal pathogen, E. vexans, was tested by detached leaf infection assay of greenhouse acclimated plantlets. An inhibitory activity against the fungal pathogen was evident from the detached leaves from the transformants compared with the control. Fungal lesion formed on control plantlet whereas the transgenic plantlets showed resistance to inoculated fungal pathogen by the formation of hypersensitivity reaction area. This result suggests that constitutive expression of the potato class I chitinase gene can be exploited to improve resistance to fungal pathogen, E. vexans, in economical perennial plantation crop like tea. © 2015, Springer-Verlag Berlin Heidelberg.


Sarma A.,Institutional Biotech Hub | Sarmah P.,Institutional Biotech Hub | Kashyap D.,Institutional Biotech Hub | Dutta S.,Institutional Biotech Hub | Mahanta M.,Institutional Biotech Hub
Journal of Pharmaceutical Sciences and Research | Year: 2015

Ethno-medicinally important plants or their parts have been used as a supplementary dietary source of nutrition and play a vital role in improving our health. Antioxidant compounds in diet play an important role as a health protecting factor. Carissa carandas L., an ethno-medicinally important plant, belongs to the family Apocynaceae and is an evergreen shrub or small crooked tree. It has been used in many ethno medicines as stomachic, anti-diarrheal and anti-helmintic and used in skin infections, fevers, rheumatism and biliary dysfunction. The present study was designed to evaluate the nutraceutical properties and antioxidant activity of the fruits of C. carandas. The antioxidant activity was evaluated by using 1,1-diphenyl-2- picrylhydrazyl (DPPH) free radicals and the IC50 value of methanolic extract of C. carandas was found to be 27.45±0.43μg/ml. Among the nutraceutical properties carbohydrate (19.32 ± 0.69 mg/100g), protein (0.185 ± 0.011μg/g), crude fibre (15.64% ±0.64), total phenolic content (188.75 ± 1.42 μgGAE/g) and ascorbic acid (62.93 ± 0.35 mg/100g) were recorded. The result shows that C. carandas have a high nutraceutical value. The study has revealed that the fruits can be used as dietary supplement as well as therapeutic agent as they would exert several beneficial effects by virtue of their antioxidant activity. © 2009 PharmaInfo Publications. All rights reserved.


Deb M.,Assam University | Adhikary P.,Institutional Biotech Hub | Slama P.,Mendel University in Brno | Havlicek Z.,Mendel University in Brno | And 3 more authors.
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis | Year: 2015

Phayre's leaf-monkeys (Trachipithecus phayrei) or Phayre's langurs are old world monkeys that inhabit South-East Asian tropical forests. The species is under a severe threat due to large scale habitat destruction and disturbances by people living near the habitat of langurs. The present study recorded the aggressive behavior of male langurs towards domestic dogs in the Cachar district of Assam. Response of each member in the troop was observed. The sophisticated behavior of males in safeguarding the weaker members was observed. In conclusion, the harassment by domestic dogs may result in the expulsion of Phayre's langurs from their native habitat.


Malakar C.,Institutional Biotech Hub | Choudhury P.P.N.,Institutional Biotech Hub | Choudhury P.P.N.,S S College
Asian Journal of Pharmaceutical and Clinical Research | Year: 2015

It has been noteworthy from the ancient times; the human race is dependent on the use of traditional plant-based medicines as well as polyherbal preparations. And from the last few decades several research works are carried out which confirms the potentiality of these natural sources as a good source of medications. Ipomoea aquatica was among such plant having good nutraceutical applications and is commonly consumed as a vegetable and is commonly found in tropical Asia, India, Africa and Australia, etc. The plant is considered to be a good source of vitamins, minerals, plant proteins, fibers, etc. as well as the plant is supposed to have tremendous pharmacological importance. The present review aims to present a brief overview of the medicinal use as well pharmacological value of the plant. © 2015 Asian Journal of Pharmaceutical and Clinical Research. All rights reserved.


Bhaigyabati T.,Institutional Biotech Hub | Devi P.G.,Imphal College | Bag G.C.,Imphal College
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2014

Hedychium species is one of the important aromatic, ornamental medicinal plants of North east India. In the present study, total flavonoid content and antioxidant activity of aqueous rhizome extract of three Hedychium species namely H. rubrum, H. coronarium and H. spicatum were investigated. In antioxidant activity: free radical scavenging activity, reducing power and total antioxidant activity were determined and expressed in terms of standard used for the respective assay. Total flavonoid content of aqueous rhizome extract of H. rubrum, H. coronarium and H. spicatum in terms of quercetin equivalent (QE) was 3.22, 2.77 and 0.74 μg/100g of extract respectively. In DPPH assay H. rubrum (32.3%) showed the highest free radical scavenging activity followed by H. coronarium (21%) and lowest activity in H. Spicatum (5.76%). In reducing power method, both standard ascorbic acid and sample extracts were found to be increased in a dose dependent manner. Total antioxidant activity of aqueous rhizome extract of H. rubrum, H. coronarium and H. spicatum in terms of ascorbic acid equivalent (AAE) was 207.3, 157.5 and 102.6 μg/ml of extract. The present data indicates that aqueous rhizome extract of all the three Hedychium species are potential source of antioxidant which may be due to the presence of flavonoid in the extracts. Among the three Hedychium species, H. rubrum was found to have the highest total flavonoid content and antioxidant activity.


Kashyap D.,Institutional Biotech Hub | Sarmah P.,Institutional Biotech Hub | Sarma A.,Institutional Biotech Hub
Research Journal of Medicinal Plant | Year: 2015

Hibiscus sabdariffa L. (Roselle; Malvaceae) has been used traditionally as food, in herbal drinks, in hot and cold beverages, as flavouring agent in the food industry and as herbal medicine. The calyx of the roselle plant has long been recognised as a source of antioxidants. The objective of this study was to evaluate antioxidant activity, free radical-scavenging and total phenolic content in calyces and epi-calyces of the roselle plant. Methanol extracts showed a positive correlation between phenolic content and antioxidant activity, as with the increase of their phenolic content (134.32±1.82 μg GAE mg-1 for the red variant and 154.60±1.54 μg GAE mg-1 for the white variant), the antioxidant activity of the of the two variants also increases (118.66±0.96 and 102.54±1.08 μg mL-1, respectively). So have the potential to be used as food antioxidants. Moreover, nutritionally both the variants are rich in protein, crude fibre, ascorbic acid. © 2015 Academic Journals Inc.


Sarmah P.,Institutional Biotech Hub | Dutta S.,Pandu College | Sarma A.,Institutional Biotech Hub
Agroforestry Systems | Year: 2016

Momordica cochinchinensis Spreng., belonging to family cucurbitaceae, is a large climber generally found under wild growing condition in the Cachar hills region of Assam, India. The fruits of this tuberous plant are supposed to possess high nutritional and medicinal properties and have been widely used in many traditional medicine practices. The present study was carried out to scientifically validate the presence of nutritional property and antioxidant activity of Momordica cochinchinensis found in this part of India. The experimental result reveals that the fruits have a high nutraceutical property and a considerable amount of antioxidative activity as the IC50 value was found to be 294.13 ± 0.46 μg/ml. © 2016 Springer Science+Business Media Dordrecht

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