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Limanjoob Reza S.,Islamic Azad University | Elham R.,Shiraz University | Nahid S.,Shiraz University | Seyed Hamid B.,Institution of Supreme Education and Industry of Maragheh | And 2 more authors.
Advances in Environmental Biology | Year: 2014

Background and objective: Providing healthy food and required for increasing population of the country, that their health is important for future physical and mental health, Including responsibilities that it should be considered by wise strategy. Among these, marines have multiple capabilities for preventing diseases and providing health and in comparison with other proteins are more completely nutrients. In this study pathogenicity of virus of white spot disease that is the cause of the death and destruction of shrimps, in the Litopenaeus vannamei have been studied experimentally. Materials and methods: In this research, 200 Litopenaeus vannamei weighing approximately 10-12 gr were randomly selected,and Infected animals being tested, and by using diagnostic kits IQ2000 PCR have been confirmed. From the frozen body of Indian white shrimps that previously have shown symptoms of white spot disease, with using Nested Polymerase chain reaction (Nested-PCR) method, the virus existence was proven, that was used as virus source, and during a day in several meals along with food was given to Litopenaeus vannamei. After 3 days, clinical symptoms including: rapid separation of the cuticle from the epidermis, empty stomach and intestines, yellow and brittle of hepatopancreas, and red moving limbs was observed. Samples of tissues from treatment groups for study PCR and next steps were selected. Results: samples with Nested-PCR method, according to instructions of diagnostic special kit of white spot disease, multiple reaction steps passed and Finally, by ultraviolet rays were observed and recorded, of which 90 samples, in 62 cases positive reactions were observed. © 2014 AENSI Publisher All rights reserved. Source


Hossein K.J.,Jahrom University of Medical Sciences | Hossein K.J.,Islamic Azad University at Jahrom | Hamid B.S.,Institution of Supreme Education and Industry of Maragheh | Roza S.,Islamic Azad University at Jahrom | And 3 more authors.
Advances in Environmental Biology | Year: 2013

Introduction: Leptin is a peptide hormone which is primarily secreted by adipose tissue, regulating the body weight, metabolism and reproductive system functioning. Stress activates hypothalamic- pituitary adrenocortical (HPA) axis that is responsible for the maintenance of homeostasis during stress. Recently, it has been found that leptin has an important role in these stress responses. Therefore, the purpose of the present investigation was to determine the effect of chronic swimming stress on plasma leptin concentratin and evaluate the role of adrenal glands in the level of leptin. Material and methods: Sixty adult male rats were enrolled in this experimental study. The rats were randomly assigned to six groups. Adrenaletomy was done in the adrenalectomized groups. Chronic stress was administered by water immersion for seven days (10 min/day). Results: Swimming Chronic stress and surgical stress cause increase and adrenalectomy causes decrease of the plasma leptin level (P<0.001). Stress had no significant effect on the plasma leptin concentration in the adrenalectomized rats. Conclusion: Chronic swimming stress activates HPA axis and, in turn, HPA activity probably stimulates leptin secretion. © 2013 AENSI Publisher All rights reserved. Source


Limanjoob Reza S.,Islamic Azad University | Jahromi Hossein K.,Jahrom University of Medical Sciences | Jahromi Hossein K.,Islamic Azad University at Jahrom | Seyed Amine D.,The Departmant General of Yasouj Province Education | And 4 more authors.
Advances in Environmental Biology | Year: 2014

Introduction: A Lierne, a Sypryns has a problem in growth carp was known for hundreds of years and are commonly called hookworm. Has a very broad area that host many members of fish has invaded freshwater eat. Surangle penetrate the fish's skin, causing a long metamorphose is a parasitic organism. The purpose of this study was to investigate the effect of hookworm on histo-pathological changes of silver carp fish and the fish is Amor. Methods: In this study on 30 samples of fish (silver carp Affairs) took 30 fish from three farms and randomly (10 examples from every field) fish weighing over 150 grams it is using inkjet net (vetch) were caught. The fish were caught in summer and the city Branch. Skin of infected fish isolated in 10% formalin buffered to a Lierne saved. To prepare slides of tissue samples for histopathology department of the Central Hospital was sent to Shiraz. The sections of providing pathology of parasitic junction Lierne A Fish specimens, the sections were studied. The results indicate that the inflammatory cell infiltration influence mononuclear cells, the formation of new fibroblasts, increased melanin pigment, muscle necrosis, congestion, de- vein, was bleeding. © 2014 AENSI Publisher All rights reserved. Source


Limanjoob Reza S.,Islamic Azad University | Roza S.,Islamic Azad University at Jahrom | Elham R.,Islamic Azad University at Jahrom | Seyed Hamid B.,Institution of Supreme Education and Industry of Maragheh | And 3 more authors.
Advances in Environmental Biology | Year: 2014

Background and objective: Providing healthy food and required for increasing population and its role in the future of a healthy physical and mental development of human society, and also new requirements of propagation and breeding shrimp by using the latest technology, new methods for researchers and experts and even authorities have opened. In this study pathogenicity of virus of white spot disease that is the cause of the death and destruction of shrimps, in the Litopenaeus vannamei have been studied experimentally. Materials and methods: In this research, 200 Litopenaeus vannamei weighing approximately 10-12 gr were randomly selected,and Infected shrimps being tested, and by using diagnostic kits IQ2000 PCR have been confirmed. From the frozen body of Indian white shrimps that previously have shown symptoms of white spot disease, with using Nested Polymerase chain reaction (Nested-PCR) method, the virus existence was proven, that was used as virus source, and during a day in several meals along with food was given to Litopenaeus vannamei. After 3 days, clinical symptoms including: rapid separation of the cuticle from the epidermis, empty stomach and intestines, yellow and brittle of hepatopancreas, and red moving limbs was observed. Samples of treatment group tissues for histopathological studies fixed in Davidson's solution, and microscopic sections prepared and by hematoxylin - eosin stained, and white spot lesions in target tissues of virus, were studied comprehensively using light microscopy. Results: From the total samples, 32 pieces showed clinical symptom of grade 1 disease severity, and 20 pieces showed grade 2 disease severity, and 14 pieces showed grade 3 disease severity, and 5 pieces showed grade 4 disease severity. In Litopenaeus vannamei, there was grade 1 and 2 of disease severity. Rate of ROI was 68/8%. © 2014 AENSI Publisher All rights reserved. Source


Sheyda A.,Islamic Azad University | Koshkaki Elham R.,Shiraz University | Houshang J.,Islamic Azad University at Jahrom | Jahromi Hossein K.,Jahrom University of Medical Sciences | And 3 more authors.
Advances in Environmental Biology | Year: 2014

Background & Aim: Based on pharmacologic classification, vitamin C is of vitamins that are solved in water. Based on treatment classification, vitamin C is of vitamins and its consumption group during pregnancy is of A. malathion is a contact insecticide that effects on pests by respiration and digestion. The aim of this study is to investigate the effect of vitamin C anti oxidant on malathion toxic effects on oogenesis and pituitary ovary hormonal axis in rats. Methods: sixty mature female rats, of wistar race were divided into six groups of ten including sham, control, vitamin C, experimental groups 1, 2, 3. The average of their weights was 200± 15 and their age was 100-120 days. Malathion poison by daily insulin syringe for 14 days in 10, 20, 40 mg/kg body weight doses and vitamin C, 20 mg/kg were injected to rats intra peritoneumly and hormonal changes LH and FSH,estrogen and progestron was studied by ELISA test. Results: the results indicated that there was no significant difference in hormone LH in groups as compared with control group and just had significant in group vitamin C (P<0.05). There was significant increase in hormone FSH in group vitamin C as compared with control group. Estrogen hormone had significant increase in group vitamin C. progestron hormone had significant decreasw in all of experimental groups and group vitamin C (P<0.05). Conclusion: vitamin C had little neutralizing effect as compared with malathion toxic effects. © 2014 AENSI Publisher All rights reserved. Source

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