ITQB UNL Institute Tecnologia Quimica e Biologica

Oeiras, Portugal

ITQB UNL Institute Tecnologia Quimica e Biologica

Oeiras, Portugal
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Alves S.P.,Ciisa Centro Interdisciplinar Of Investigacao Em Sanidade Animal | Bessa R.J.B.,Ciisa Centro Interdisciplinar Of Investigacao Em Sanidade Animal | Quaresma M.A.G.,Ciisa Centro Interdisciplinar Of Investigacao Em Sanidade Animal | Milton J.,University of Western Australia | And 4 more authors.
PLoS ONE | Year: 2013

Fat tailed sheep breeds are known for their adaptation to nutritional stress, among other harsh production conditions. Damara sheep, native to Southern Africa, have recently been exported to other areas of the world, particularly Australia, aiming to produce lamb in semi-arid regions. Damaras have a unique hanging fat tail, a fat depot able to be mobilized under nutritional stress. In this article we perform an in-depth characterization of the fatty acid profiles of the fat tail in underfed and control Damara rams. Profiles were very similar between experimental groups, with the exception of palmitic acid (16:0) that was lower (P = 0.014) in underfed animals. However, the most striking result was the very high proportions of non-terminal branched chain fatty acids found in the fat tail adipose tissue, as well as the gastrocnemius muscle of Damara rams. The muscle of Dorper and Merino rams used in the same experiment did not present non-terminal branched chain fatty acids, suggesting that Damara rams have a unique lipid metabolism. Herein, we interpret this trait relating it to a higher ability of Damara sheep to digest fibrous fodder and to putative differences in the propionate metabolism by comparison to other sheep breeds. © 2013 Alves et al.


Paredi G.,University of Parma | Sentandreu M.-A.,CSIC - Institute of Agricultural Chemistry and Food Technology | Mozzarelli A.,University of Parma | Mozzarelli A.,Italian National Institute of Biosystems and Biostructures | And 4 more authors.
Journal of Proteomics | Year: 2013

Meat consumption is an important part of human diet with strong implications in health, economy and culture worldwide. Meat is a proteinaceous product and therefore proteomics holds a considerable value to the study of the protein events underlying meat production and processing. In this article we will review this subject in an integrated "farm to fork" perspective, i.e. focusing on all the major levels of the meat producing chain: farm, abattoir and transformation industry. We will focus on the use, importance and applications of proteomics, providing clear examples of the most relevant studies in the field. A special attention will be given to meat production, as well as quality control. In the latter, a particular emphasis will be given to microbial safety and the detection of frauds.This article is part of a Special Issue entitled: New Horizons and Applications for Proteomics [EuPA 2012]. © 2013 Elsevier B.V.


Hernandez-Castellano L.E.,University of Bern | Ferreira A.M.,ITQB UNL Institute Tecnologia Quimica e Biologica | Ferreira A.M.,ICAAM Institute Ciencias Agrarias e Ambientais Mediterranicas | Nanni P.,ETH Zurich | And 9 more authors.
Journal of Proteomics | Year: 2016

Seasonal weight loss (SWL) is a significant limitation to animal production. Breeds that have evolved in harsh climates have acquired tolerance to SWL through selection. Herein, label free proteomics was used to characterize the effects of SWL in two goat breeds with different levels of adaptation to nutritional stress: Majorera (tolerant) and Palmera (susceptible). Nine Majorera and 10 Palmera dairy goats were used, divided in 4 sets, 2 for each breed: underfed and a control group: Majorera Control (MC), Palmera Control (PC), Majorera Restricted (ME) and Palmera Restricted (PE). At day 22, mammary gland biopsy samples were obtained. Label free proteomic analysis enabled the identification of over 1000 proteins, of which 96 showed differential expression between two of the groups within studied comparisons. Majorera breed showed higher expression of immune system related proteins. In contrast, Palmera breed showed higher expression of proteins related to apoptosis. Results indicate that the two goat breeds have a distinct metabolism reaction to SWL, and that proteins related to the immune system and apoptosis such as cadherin-13, collagen alpha-1, nidogen-2, clusterin and protein s100-A8 could be considered putative candidates as markers of tolerance to SWL. Biological significance: Seasonal weight loss (SWL) is one of the major constraints to animal production in the tropics. We compared the proteomics profiles of two dairy goat breeds with different levels of tolerance to SWL under control and feed restriction conditions using label free proteomics. We have identified over 1000 proteins in the goat mammary gland, of which 96 showed differential expression. Despite the fact that both breeds showed a decrease in the number of proteins related to protein, carbohydrates and fat biosynthesis, the tolerant breed showed higher expression of immune system related proteins compared to the susceptible breed. On the contrary, the susceptible breed had higher expression of apoptosis related proteins indicating that both breeds reacted differently to weight loss and that proteins related to the immune system and apoptosis such as cadherin-13, collagen alpha 1, nidogen-2 or clusterin may be suggested as markers of tolerance to SWL. © 2016 Elsevier B.V.

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