Institute Tecnologia Agraria

Valladolid, Spain

Institute Tecnologia Agraria

Valladolid, Spain
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Yahiaoui S.,CSIC - Aula Dei Experimental Station | Yahiaoui S.,French National Institute for Agricultural Research | Cuesta-Marcos A.,CSIC - Aula Dei Experimental Station | Cuesta-Marcos A.,Oregon State University | And 9 more authors.
Plant Breeding | Year: 2014

Barley landraces from the western Mediterranean area have not been thoroughly exploited by modern breeding. This study aims at assessing the agronomic value of a core collection of lines derived from landraces of Spanish origin and to compare them with sets of successful old and modern cultivars. The agronomic performance of a set of 175 barley genotypes, comprising 159 landrace-derived lines and 26 cultivars, was evaluated in a series of 10 field trials, carried out over 3 years and several locations. The most relevant trait of the landraces was higher grain yield at low production sites than cultivars, which may be related with better ability to fill the grain under stressful conditions. On the other hlateness, excessive plant height and lodging were negative traits frequently found in the landraces. Large genotype-by-environment interaction (GEI) for grain yield was detected, related partly with differences between germplasm groups, probably indicating local adaptation. GEI was also associated with the interaction of heading time and powdery mildew resistance with temperature. © 2014 Blackwell Verlag GmbH.

Casas A.M.,CSIC - Aula Dei Experimental Station | Djemel A.,CSIC - Aula Dei Experimental Station | Ciudad F.J.,Institute Tecnologia Agraria | Yahiaoui S.,CSIC - Aula Dei Experimental Station | And 6 more authors.
Theoretical and Applied Genetics | Year: 2011

In barley, three genes are responsible for thevernalization requirement: VrnH1, VrnH2 and VrnH3. Thewinter growth habit of barley requires the presence of arecessive VrnH1 allele, together with an active VrnH2allele. The candidate for VrnH3 (HvFT1) has been recentlyidentiWed, with evidences pointing at a central role in theintegration of the vernalization and photoperiod pathways.Functional polymorphisms have been proposed, but experimentalevidence of their role on agronomic performanceand adaptation is needed. We examined allelic variation atthe promoter and intron 1 of the HvFT1 gene in a landracecollection of barley, Wnding a high diversity level, with itsgeographic distribution correlated with latitude. Focusingon genotypes with winter alleles in VrnH1 and VrnH2, anassociation analysis of the four main HvFT1 haplotypesfound in the landrace collection detected diVerences in timeto Xowering. Landraces with the intron 1 TC allele, prevalentin the south, Xowered 6-7 days earlier than those withthe AG allele, under natural conditions. These results werevalidated in an independent F2 population. In both data sets,the eVect found was similar, but in opposite direction tothat described in literature. The polymorphism reported atintron 1 contributes to variation in Xowering time underWeld conditions. We have found that polymorphisms at thepromoter also contribute to the eVect of the gene on Xoweringtime under Weld and controlled conditions. The varietyof HvFT1 alleles described constitutes an allelic series thatmay have been a factor in agro-ecological adaptation ofbarley. © Springer-Verlag 2011.

Silvar C.,CSIC - Aula Dei Experimental Station | Casas A.M.,CSIC - Aula Dei Experimental Station | Kopahnke D.,Julius Kuhn Institute | Habekuss A.,Julius Kuhn Institute | And 7 more authors.
Plant Breeding | Year: 2010

The Spanish Barley Core Collection comprises 159 landrace-derived inbred lines and 16 cultivars adapted to Southern European conditions. The collection was screened for resistance to powdery mildew (Blumeria graminis), scald (Rhynchosporium secalis), leaf rust (Puccinia hordei), net blotch (Pyrenophora teres f. teres), Barley yellow dwarf virus (BYDV) and Barley mild mosaic virus (BaMMV). Resistance to powdery mildew was outstanding, with 58 lines presenting mean overall resistance, among them seven landrace-derived lines resistant to all seven isolates tested. About 26% of the Spanish lines were resistant to scald. Resistance to leaf rust and to net blotch was scarce, though a few accessions showed resistance levels as good as the checks. Thirteen accessions (12 Spanish) were totally resistant to BaMMV and c. 20% of accessions showed moderate tolerance to BYDV. Landrace-derived lines from the Mediterranean Coast and Southern regions of Spain were the most resistant to powdery mildew and leaf rust, but the most susceptible to viruses. Potential sources of resistance might be preserved in some accessions subjected to selective pressure in the region of origin. © 2009 Blackwell Verlag GmbH.

Igartua E.,CSIC - Aula Dei Experimental Station | Mansour E.,CSIC - Aula Dei Experimental Station | Mansour E.,Zagazig University | Cantalapiedra C.P.,CSIC - Aula Dei Experimental Station | And 10 more authors.
Molecular Breeding | Year: 2015

This study is a retrospective analysis of an elite cross from the Spanish National Barley Breeding Program. This was the most successful cross produced in the breeding program in the past 20 years. The progeny from this cross has been investigated at two points in the program, before and after selection, through the analysis of allelic frequencies at a number of genetic loci with molecular markers. Shifts in allelic frequencies after selection allowed the identification of genomic regions with selection footprints likely due to the breeding process. The cross was replicated in three different years, and therefore, the three progenies represent different selection histories but, in all cases, were preferentially selected compared to the lines from other crosses used in the program. The progenies were sampled at two generations, before conscious selection (F2) and after six generations of selection (F8). The F2 plants were genotyped with microsatellites, whereas 31 F8 lines were surveyed for SNP and presence/absence variation polymorphisms using a genotyping-by-sequencing system (DArTseq). The DArTseq markers were aligned to the barley physical map, and, after curation, over 3,000 were still available for the analysis. Overall, 15 genomic regions in the F8 lines had allele frequencies beyond chosen thresholds, indicating selection, eight toward parent Orria and seven toward Plaisant. These selection footprints partially validated QTLs detected through classical linkage mapping in a RIL population of the same cross. These validated selection footprints convey useful information for barley breeding, either through marker-assisted selection or through genomic selection. © 2015, Springer Science+Business Media Dordrecht.

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