Institute Superieur Of Biotechnologie Of Monastir Isbm

Monastir, Tunisia

Institute Superieur Of Biotechnologie Of Monastir Isbm

Monastir, Tunisia

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Nabli R.,Institute Superieur Of Biotechnologie Of Monastir Isbm | Nabli R.,Center de Biotechnologie de la Technopole de Borj-Cedria | Achour S.,Institute Superieur Of Biotechnologie Of Monastir Isbm | Jourdes M.,CNRS Oenologie Research Unit | And 5 more authors.
Journal International des Sciences de la Vigne et du Vin | Year: 2012

Aim: Anthocyanins are water soluble pigments mainly located in grape skin; however, these phenolic compounds are also located in vine leaves. The aim of this work was to identify, quantify and determine for the first time the anthocyanin composition in Grenache noir (Vitis vinifera) leaves. Methods and results: Five anthocyanins were identified and quantified in Grenache noir leaves by HPLC-UV-MS. Of these, cyanidin-3-O-glucoside and peonidin-3-O- glucoside were the main anthocyanins and represented 44 and 37%, respectively, while delphinidin-3-O-glucoside, petunidin-3-O-glucoside and malvidin-3-O-glucoside accounted for only 6,5 and 8%, respectively. A multi-factorial design experiment was used to build a mathematical model to estimate the best extraction condition (highest anthocyanin extraction yield from leaf extract) for both SO2 and ethanol in the aqueous extraction solvent For SO2, the optimal extraction parameters were an extraction time ranging between 5.77 and 6 h, a temperature ranging between 20 and 23.7°C and a concentration of 500 ppm of SO2 in the aqueous extraction solvent Using the hydro-alcoholic extraction solvent, the optimal extraction parameters were an extraction time ranging between 3 and 4.37 h, a temperature set at 20°C and an ethanol concentration in the extraction solvent ranging between 32 and 40%. Conclusion: Only the five mono-glucoside anthocyanins usually detected in grapes have been detected, indentified and quantified in Grenache noir leaves by HPLC-UV-MS. Using a multi-factorial design experiment the optimum conditions for the extraction of these anthocyanins were obtained in hydro-alcoholic solution (i.e., extraction time ranging between 3 and 431 h, a temperature set at 20°C and an ethanol concentration ranging between 32 and 40%) and in water with SO 2 (i.e., extraction time ranging between 5.77 and 6 h, a temperature ranging between 20 and 23.7°C and a concentration of500 ppm of SO2 in water). Significance and impact of the study: Five anthocyanins were detected, identified and quantified in Grenache noir leaves by HPLC-UV-MS and the main anthocyanin were with cyanidin-3-O-glucoside and peonidin-3-O-glucoside. The optimum conditions for the extraction of these anthocyanins were estimated in hydro-alcoholic solution and in water solvent with SO2 which will allow further investigation on these anthocyanins to be used as natural pigment for the food as well as for the pharmaceutical and cosmetic industry. © Vigne et Vin Publications Internationales (Bordeaux, France).


Nabli R.,Institute Superieur Of Biotechnologie Of Monastir Isbm | Nabli R.,Center de Biotechnologie de la Technopole de Borj-Cedria | Achour S.,Institute Superieur Of Biotechnologie Of Monastir Isbm | Jourdes M.,CNRS Oenologie Research Unit | And 5 more authors.
Journal International des Sciences de la Vigne et du Vin | Year: 2013

Aim : Anthocyanins are water soluble pigments located in grape skin as well as in vine leaves. The aim of this work was to identify, and determine the anthocyanin composition in Grenache noir (Vitis vinifera) leaves as well as to estimate the optimum conditions leading to the highest anthocyanin extraction yield. Methods and results : Five anthocyanins were identified and quantified in Grenache noir leaves by HPLC-DAD-MS. Among these anthocyanins, cyanidin-3-O-glucoside and peonidin-3-O-glucoside were the main anthocyanins, representing 43 and 38%, respectively, while delphinidin-3-O-glucoside, petunidin-3-O-glucoside and malvidin-3-O-glucoside accounted for only 6, 5 and 8%, respectively. To estimate the highest anthocyanin extraction yield, a multi-factorial design experiment was used to build a mathematical model. The extraction conditions tested were time, temperature and solvent composition (i. e., ethanol or SO2 level in acidified water). Conclusion : Only the five mono-glucoside anthocyanins usually detected in grapes have been detected, identified and quantified in Grenache noir leaves. The optimum extraction conditions of these anthocyanins were obtained for acidified hydro-alcoholic solution (i. e., pH 2, 3 h, temperature ranging between 36.5 and 40 °C and an ethanol concentration ranging between 36.8 and 40%) and for acidified water with SO2 (i. e., pH 2, between 5.8 and 6 h, a temperature ranging between 38.6 and 40 °C and a concentration of 500 ppm of SO2). Significance and impact of the study : Five mono-glucoside anthocyanins were identified and quantified in Grenache noir leaves. The optimum extraction conditions for of these anthocyanins were estimated in acidified hydro-alcoholic solution and in acidified water with SO2. These extraction procedures will allow further investigation of the potential use of these anthocyanins as natural pigment for food as well as for the pharmaceutical and cosmetic industry. ©Vigne et Vin Publications Internationales (Bordeaux, France).


Zouiten D.,Institute National Des Science Et Technologies Of La Mer Instm | Ben Khemis I.,Institute National Des Science Et Technologies Of La Mer Instm | Slaheddin Masmoudi A.,Institute Superieur Of Biotechnologie Of Monastir Isbm | Huelvan C.,French Research Institute for Exploitation of the Sea | Cahu C.,French Research Institute for Exploitation of the Sea
Aquaculture Research | Year: 2011

The quality of development in intensive or mesocosm hatchery-reared Dicentrarchus labrax larvae was investigated using physiological indicators assessing ontogeny. Larvae were reared in intensive (120L tanks) and in mesocosm systems (20m3 enclosures) with the same feeding sequence, excluding the wild zooplankton naturally available in mesocosms. Faster growth was recorded since early development [16 day after hatching (DAH)] in the mesocosm. Maturation of the digestive system also occurred earlier as indicated by the higher amylase secretion ratios, the intestinal maturation index (alkaline phosphatase/leucine-alanine peptidase and aminopeptidase-N/leucine-alanine peptidase ratios) and the more developed intestinal epithelium at 23DAH. Nevertheless, the delay in digestive maturation in the intensive system seemed retrieved within few days. In both the groups, the number of vertebrae ranged between 24 and 26, with the dominant class being 25 vertebrae. However, the distributions differed between treatments for meristic characteristics, ossification stages and incidence of malformation types. Loss of a vertebra was more frequent in the intensive system, while the appearance of an additional vertebra was more frequent in the mesocosm. Ossification at 37DAH was also more advanced in the mesocosm in addition to a lesser rate and severity of skeletal malformations. It is suggested that the early nutritional contribution of mesocosm wild zooplankton, yet at densities of 0.2-0.7preymL-1, had key effects on larvae development since the early stages. © 2011 Blackwell Publishing Ltd.


Kabbage M.,University of Monastir | Trimeche M.,University of Sousse | Bergaoui S.,University of Monastir | Hammann P.,French National Center for Scientific Research | And 6 more authors.
Tumor Biology | Year: 2013

The aim of this study was to evaluate calreticulin expression in infiltrating ductal breast carcinomas (IDCAs), as well as its relationships with clinicopathological parameters of the disease. Using a two-dimensional gel electrophoresis/matrix-assisted laser desorption ionization time of flight mass spectrometry investigation coupled to an immunohistochemical approach, we have assessed the expression of calreticulin in IDCAs, as well as in other types of breast tumors. The humoral immune response against calreticulin was estimated using a serological proteomics-based strategy. Proteomic analyses revealed an increased expression of calreticulin in IDCA tumors. Using immunohistochemistry, overexpression of calreticulin was confirmed in 51 additional tumor specimens. Statistical analyses revealed, however, no significant correlations between calreticulin expression and clinicopathological parameters of the disease including tumor stage, patient age, SBR grade, and lymph node metastasis occurrence. A significant association was found, however, with estrogen receptor status. This study demonstrates the upregulation of calreticulin in IDCA tissues which may highlight its involvement in breast cancer development. Our findings also support a link between calreticulin expression and estrogen transduction pathways. Our results do not, however, support the involvement of calreticulin in the development of a humoral immune response in IDCAs. © 2013 International Society of Oncology and BioMarkers (ISOBM).

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