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Ben Ghnaya A.,University of Tunis | Ben Ghnaya A.,University of Carthage | Amri I.,University of Carthage | Hanana M.,Center de Biotechnologie de la Technopole de Borj-Cedria | And 4 more authors.
Industrial Crops and Products | Year: 2016

Our study is in line with the valorization of Tunisian medicinal and aromatic plants in order to discover new bioactive natural products. So the aim was to characterized the physico-chemical properties and yield of the essential oil obtained by hydrodistillation from the leaves of Tunisian Tetraclinis articulata (Vahl.) Masters. Thirty one compounds were identified using gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS): they accounted for 91% of the essential oil composition with a majority of monoterpenes hydrocarbons (62.80%), in particular α-pinene (56.21%) and β-myrcene (3.08%). Further investigations allowed the identification of oxygenated monoterpenes, which represented 18.98%, with 1,8-cineole as the major compound of this fraction. The oil antifungal activity was assessed towards five phytopathogenic fungi. The strongest activity was observed against Botrytis cinerea with 71.17% inhibition. The oil herbicidal properties were tested on Sinapis arvensis L. and Phalaris canariensis L. Results indicated that T. articulata essential oil completely inhibited the seed germination of S. arvensis L. at high concentration (4. μl/ml), while at low doses (1 and 2. μl/ml), it delayed the germination and reduced the seedling growth of both weeds tested. Indeed, the use of these essential oils causes inhibition of weeds germination by setting their vegetative growth. © 2016.


Ben Ghnaya A.,University of Tunis | Hanana M.,Center de Biotechnologie de la Technopole de Borj-Cedria | Amri I.,Institute National Of Recherches En Genie Rural | Balti H.,Institute National Of Recherches En Genie Rural | And 3 more authors.
Journal of Pest Science | Year: 2013

The chemical composition of essential oil isolated by hydrodistillation from the leaves of Tunisian Eucalyptus erythrocorys Linn. was analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS). Fifteen compounds were identified, representing 97 % of total oil, which was found to be rich in oxygenated monoterpenes (66.7 %), particularly in 1,8-cineole (54.8 %). The yield and the physico-chemical properties of oil were determined. The study of antifungal activity revealed that E. erythrocorys essential oil significantly inhibited the growth of five plant pathogenic fungi especially Bipolaris sorikiniana and Botritys cinerea. Moreover, herbicidal properties of the oil, tested on Sinapis arvensis L. and Phalaris canariensis L. indicated that the E. erythrocorys oil completely inhibited the seed germination at high concentration (1.5 μl/ml), while at low doses (0.25, 0.5, 0.75, 1 μl/ml) the oil acted by decreasing and delaying the germination and inhibiting the seedling growth of all tested weeds. © 2013 Springer-Verlag Berlin Heidelberg.


Amri I.,Zarzouna | Hanana M.,Center de Biotechnologie de la Technopole de Borj-Cedria | Gargouri S.,French National Institute for Agricultural Research | Jamoussi B.,Institute Superieur Deducation Et Of Formation Continue | Hamrouni L.,Institute National Of Recherches En Genie Rural
Chilean Journal of Agricultural Research | Year: 2013

Maritime pine (Pinus pinaster Aiton) and Saharan cypress (Cupressus sempervirens L. var. dupreziana [A. Camus] Silba) are two cone-bearing seed coniferous woody plants. The chemical composition of their essential oils, isolated from needles and leaves by hydrodistillation, was analyzed with gas chromatography (GC) and gas chromatography mass spectrometry (GC/MS). A total of 66 and 28 compounds were identifed, which represented 99.5% and 98.9% of total pine and cypress oils, respectively. Pinus pinaster oil was found to be rich in α-pinene (31.4%), (Z)-caryophyllene (28%), and α-humulene (6.7%); it was characterized by relatively high amounts of monoterpene and sesquiterpene hydrocarbons (44.5% and 46.3%, respectively). The major components identified in cypress oil were manoyl oxide (34.7%), α-pinene (31.8%), α-humulene (9%), and δ-3-carene (8.7%). Results of in vitro antifungal test assays showed that both oils signifcantly inhibit the growth of 10 plant pathogenic fungi. Herbicidal effects of the oils on seed germination, seed vigor, and seedling growth of three common crop weeds Sinapis arvensis L., Phalaris paradoxa L., and Raphanus raphanistrum L. were also determined; the oils completely inhibited seed germination and seedling growth of all the weeds.


Amri I.,University of Carthage | Amri I.,Institute National Of Recherches En Genie Rural | Amri I.,Center de Biotechnologie de la Technopole de Borj-Cedria | Amri I.,Institute Superieur Deducation Et Of Formation Continue | And 2 more authors.
Chilean Journal of Agricultural Research | Year: 2014

The aims of this research were to determine the chemical composition of the essential oil of three Tunisian plants and to evaluate their biological activity against eggs, larvae, and adult insects of Ectomyelois ceratoniae Zeller. The essential oils extracted from leaves of Thymus capitatus (L.) Hoffmanns. & Link, Rosmarinus officinalis L. and needles of Pinus halepensis Mill. were analyzed by gas chromatography-mass spectrometry; 34, 16, and 56 constituents were identified, respectively. The major constituents were (Z)-caryophyllene (23.8%), β-myrcene (20.5%) and α-pinene (13.3%) in P. halepensis oil, carvacrol (66.9%), p-cymene (9.1%), and δ-terpinene (6.2%) in T. capitatus oil and 1, 8-cineole (47.5%), camphor (14.9%), α-pinene (14.1%), and borneol (13.1%) in R. officinalis oil. The insecticidal effects of essential oils on eggs, larvae, and adults of E. ceratoniae were investigated. Ovicidal activity of oils was studied by spray on eggs while larvicidal and adulticidal activities were assessed by fumigation and spray. Number of hatched eggs was verified after 10 d, larva and adult mortalities were observed after 6, 12, and 24 h. Globally, eggs and larvae were the most resistant to the three different oils, needing higher doses to obtain a higher mortality. The spray method was most effective than fumigation. Essential oil extracted from T. capitatus proved to be very toxic towards E. ceratoniae on all three phases at the dose of 20 μL mL-1 (100% inhibition), followed by the oil from R. officinalis (90-100% inhibition), nevertheless, weak activity was obtained with P. halepensis oil (68.3-85% inhibition). Results obtained may suggest that the essential oils of T. capitatus and R. officinalis possess high insecticidal activity and therefore, can be used in biotechnological application as natural preservative in stored dates and could be useful in managing populations of E. ceratoniae in field. ©2014, Instituto de Investigaciones Agropecuarias, INIA. All rights reserved.


Metoui N.,Center technique des agrumes | Gargouri S.,French National Institute for Agricultural Research | Amri I.,Institute National Of Recherches En Genie Rural | Fezzani T.,Institute National Of Recherches En Genie Rural | And 2 more authors.
Natural Product Research | Year: 2015

Our study is about the essential oil of Citrus aurantium L. in Tunisia and its plant extract. The yield of this essential oil is 0, 56% but the yield of the extract of plant was 17.1% for the aqueous extract ant 18.3% for the ethanolic extract. The analysis of chemical composition by using GC and GC/MS showed the essential oil of C. aurantium L. species to be rich in monoterpenes such as α-terpineol, lianolyl acetate, linalool and limonene. The antifungal activity of this oil showed us an inhibition of the germination of mushrooms, in the same way we could note that the biologic activities are generally assigned to the chemotypes high content in oxygenated monoterpene. © 2015 Taylor & Francis


Amri I.,University of Carthage | Amri I.,Institute National Of Recherches En Genie Rural Eaux Et Forets | Lamia H.,Institute National Of Recherches En Genie Rural Eaux Et Forets | Gargouri S.,French National Institute for Agricultural Research | And 5 more authors.
Natural Product Communications | Year: 2011

Essential oils isolated from needles of Pinus patula by hydrodistillation were analyzed by gas chromatography-flame ionization detection (GC-FID) and gas chromatography mass spectrometry (GC-MS). Thirty-eight compounds were identified, representing 98.3% of the total oil. The oil was rich in monoterpene hydrocarbons (62.4%), particularly α-pinene (35.2%) and β-phellandrene (19.5%). The in vitro antifungal assay showed that P. patula oil significantly inhibited the growth of 9 plant pathogenic fungi. The oil, when tested on Sinapis arvensis, Lolium rigidum, Phalaris canariensis and Trifolium campestre, completely inhibited seed germination and seedling growth of all species. Our preliminary results showed that P. patula essential oil could be valorized for the control of weeds and fungal plant diseases.

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