Acosta G.,Area de Quimica Analitica |
Acosta G.,Institute Quimica Of San Luis Cct San Luis |
Silva R.,National University of San Luis |
Gil R.A.,Area de Quimica Analitica |
And 5 more authors.
Talanta | Year: 2013
Multiwalled carbon nanotubes are evaluated here as solid phase extraction (SPE) sorbent aiming to (±)-chlorpheniramine (CPA) enantioresolution with fluorimetric detection. β-cyclodextrin (CD) was added to the racemate and solutions with HCl and sodium dodecyl sulfate (SDS) in different proportions were assayed as eluents to achieve the separation between both enantiomers. The overall methodology involved a flow injection (FI) strategy enabling high sample throughput and low reagents consumption making it suitable for drug routine quality control. An adequate enantioresolution (2.08) with satisfactory responses for both (R)-CPA (peak area=285) and (S)-CPA (peak area=380) was achieved applying the proposed FI-SPE strategy under the optimized conditions [β-CD]=1.0 mmol L-1, [HCl]=1.0×10-2 mol L -1, [SDS]=4.0×10-4 mol L-1 and eluent flow rate=8.0 rpm. © 2012 Published by Elsevier B.V.
Verni E.R.,Institute Quimica Of San Luis Cct San Luis |
Moyano F.,Institute Quimica Of San Luis Cct San Luis |
Martinez L.D.,Institute Quimica Of San Luis Cct San Luis |
Lapierre A.V.,Institute Quimica Of San Luis Cct San Luis |
Gil R.A.,Institute Quimica Of San Luis Cct San Luis
Journal of Analytical Atomic Spectrometry | Year: 2013
Inductively coupled plasma mass spectrometry instrumentation along with the dynamic reaction cell (DRC) technology has been increasingly used in the last decade for multielemental analysis of biological samples. This work reports the development of a method to assess concentrations of Li, Be, B, Sc, Mn, Fe, Co, Ni, Cu, Zn, Ga, Ge, As, Se, Rb, Cd, Sr, Mo, Pd, Ag, Sb, Te, Cs, Ba, Os, Tl, Pb, and Bi in human serum samples treated with formic acid. This is a single-step procedure that minimizes sample handling and avoids contamination risks and analyte losses. Towards this aim, several experimental conditions such as sample pretreatment with formic acid and instrumental operating parameters were optimized. Interferences due to polyatomic ions were eliminated using DRC with oxygen as reaction gas through the formation of MO+ ions. Our method involved low dilution factors, and appropriate limits of detection in the low μg L-1 range for all elements. The accuracy was evaluated through the analysis of a standard reference material (Inorganic Constituents in Animal Serum, NIST 1598a) and spiked samples, with satisfactory recoveries. © The Royal Society of Chemistry 2013.