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Medellín, Colombia

Liu Y.,Imperial College London | Childs R.A.,Imperial College London | Palma A.S.,Imperial College London | Palma A.S.,New University of Lisbon | And 5 more authors.
Methods in Molecular Biology

Carbohydrate microarrays, since their advent in 2002, are revolutionizing studies of the molecular basis of protein-carbohydrate interactions both in endogenous recognition systems and pathogen-host interactions. We have developed a unique carbohydrate microarray system based on the neoglycolipid (NGL) technology, a well-validated microscale approach for generating lipid-tagged oligosaccharide probes for use in carbohydrate recognition studies. This chapter provides an overview of the principles and key features of the NGL-based oligosaccharide microarrays, and describes in detail the basic techniques - from the preparation of NGL probes to the generation of microarrays using robotic arraying hardware, as well as a general protocol for probing the microarrays with carbohydrate-binding proteins. © 2012 Springer Science+Business Media, LLC. Source

Torres-Palma R.A.,Institute Quimica | Torres-Palma R.A.,University of Savoy | Torres-Palma R.A.,Ecole Polytechnique Federale de Lausanne | Nieto J.I.,Ecole Polytechnique Federale de Lausanne | And 3 more authors.
Water Research

This paper explores the degradation of a model pollutant, bisphenol A, by an advanced oxidation process that combines sonolysis, Fe2+, and TiO2 in a photoassisted process. Experiments were done under saturated oxygen conditions. The effect of different Fe2+ (0.56 and 5.6 mg/L) and TiO2 (10 and 50 mg/L) concentrations was investigated on both the elimination and mineralization of the pollutant. A pronounced synergistic effect that led to the complete and rapid elimination of dissolved organic carbon (DOC) was observed even at low catalyst loadings. In this system, almost a complete removal of DOC (93%) was observed after 4 h using 10 and 5.6 mg/L of TiO2 and Fe2+, respectively, whereas at the same time, only 5, 6, and 22% of DOC was removed by an individual process alone (TiO2 photocatalysis, ultrasound, and photo-Fenton, respectively). In this system, ultrasound has the principal role of eliminating the initial substrate and providing hydrogen peroxide for the photocatalytic systems, while photo-Fenton and TiO2 photocatalysis are mainly responsible for the transformation of the intermediates in CO2 and H2O. The role of H2O2 generated from the sonochemical process is also discussed. © 2010 Elsevier Ltd. All rights reserved. Source

Minutolo P.,CNR Institute for Research on Combustion | Commodo M.,CNR Institute for Research on Combustion | Santamaria A.,CNR Institute for Research on Combustion | Santamaria A.,Institute Quimica | And 2 more authors.

Two-dimensional carbon nanostructures have been produced via flame synthesis. Flame conditions and sampling methods have been optimized for the collection of the carbon nanostructures and for their characterization. Atomic Force Microscopy, Differential Mobility Analysis and Raman spectroscopy have been used to characterize the morphology and the chemical composition of these carbon nanostructures. Results show that a network of aromatic compounds connected by non-aromatic bonding arrange to form bi-dimensional structures that assume an atomically thin disk-like shape when deposited on a substrate. The in plane lateral dimension is of the order of tens of nanometers, and the height of about 3 Å. In spite of a significant amount of disorder, Raman spectroscopy evidences that lattice distortion is not very large in these flame nano-disks. © 2013 Elsevier Ltd. All rights reserved. Source

Filho C.V.S.M.,Federal University of Bahia | Dos Santos W.P.C.,Federal University of Bahia | De Kikuchi R.K.P.,Federal University of Bahia | Hatje V.,Institute Quimica
Microchemical Journal

The three most used decontamination procedures for coral samples were evaluated in order to subsidize the development of a simpler, faster and more efficient cleaning procedure for decontaminating coral skeleton for trace element determinations using ICP OES. The procedures tested involved a sequence of ultrasonic cleaning with deionized water, 0.2mol.L -1 HNO 3 and/or an oxidizing mixture of 30% H 2O 2 and 0.2mol.L -1 NaOH in an ultrasonic bath to remove particles and residues from saw blades used during the collection and pretreatment of coral samples. The main contaminants identified were Fe, Cu and Zn. The three decontamination procedures tested were efficient, but indicated that the decontamination steps with deionized water are unnecessary. The procedure proposed in this study proved to be more efficient, as only one extracting agent was used, the number of steps required to decontaminate the coral samples was reduced, consequently saving time and increasing analytical frequency. © 2011 Elsevier B.V. Source

Martinez-Vazquez M.,Institute Quimica
Biochemical Systematics and Ecology

A chemical analysis of Ibervillea lindheimeri (A. Gray) Greene led to the isolation of 23,24-dihydrocucurbitacin F (1), 23,24-dihydrocucurbitacin D (2) and 2-O-β-D-glucopyranosyl 23,24-dihydrocucurbitacin D (3). Their structures were identified by spectroscopic analyses and by comparison of their spectral data with those reported in the literature. In addition, 10 compounds were detected by GC-MS analyses from the hexane extract. To the best of our knowledge, this is the first report examining the chemical composition of I. lindheimeri. © 2014 Elsevier Ltd. Source

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