Janeth Colina R.,Central University of Venezuela |
Adriana Mendez O.,Central University of Venezuela |
Humberto Araque M.,Central University of Venezuela |
Humberto Araque M.,Institute Produccion Animal |
And 5 more authors.
Revista MVZ Cordoba | Year: 2011
Objetive. Two experiments were designed to evaluate the effect of peach palm meal and synthetic lysine on blood lipids of growing and finishing pigs. Materials and methods. In experiment 1, 72 barrows of 30 ± 0.5 kg were randomly allotted into a 2x3 factorial design: being the main factors levels of synthetic lysine (0 and 2.70 g/kg) and levels of peach palm meal (0, 160 and 320 g/kg). In experiment 2, 16 finishing pigs of 67.25 ± 1.17 kg were used and allotted in a 2x2 factorial design arrangement of treatments: with two levels of synthetic lysine (0 and 2.70 g/kg) and two levels of peach palm meal (0 and 175 g/kg). Serum triglycerides, total cholesterol and fatty acid profile were determined. Results. In experiment 1, growing pigs fed with 160 and 320 g/kg of peach palm showed lower (p<0.001) cholesterol (2.27 and 2.23 mmol/l, respectively) than the control (2.56 mmol/l). The levels of triglycerides were also lower in pigs fed with 160 and 320 g/kg (0.34 and 0.28 mml/l) than control group (0.42 mmol/l). Oleic acid concentration increased (p<0.01) in pigs that received the highest level of peach palm (320 g/kg) with respect to the control group (20.78% to 28.84%) and synthetic lysine increased (p<0.05) linoleic acid (27.83% to 31.29%). Pigs fed peach palm and lysine had lower (p<0.001) palmitic acid concentration than the group fed pijiguao without lysine (0.23% and 0.19% vs 0.45% and 0.62%, respectively). In experiment 2, triglycerides decreased (p<0.05) in pigs that received lysine and peach palm (0.46 to 0.36 mmol/l). Pigs fed with peach palm had lower linoleic acid and higher oleic acid (p<0.001). Conclusions. Diets with peach palm and synthetic lysine do not elicit detrimental effects on blood lipid profile in pigs.
Martinez A.M.,University of Cordoba, Spain |
Martinez A.M.,Northeast National University |
Periati V.L.,University of Cordoba, Spain |
Bermejo J.V.D.,University of Cordoba, Spain |
And 31 more authors.
Journal of Animal Science | Year: 2014
Little is known about local Criollo pig genetic resources and relationships among the various populations. In this paper, genetic diversity and relationships among 17 Criollo pig populations from 11 American countries were assessed with 24 microsatellite markers. Heterozygosities, F-statistics, and genetic distances were estimated, and multivariate, genetic structure and admixture analyses were performed. The overall means for genetic variability parameters based on the 24 microsatellite markers were the following: mean number of alleles per locus of 6.25 ± 2.3; effective number of alleles per locus of 3.33 ± 1.56; allelic richness per locus of 4.61 ± 1.37; expected and observed heterozygosity of 0.62 ± 0.04 and 0.57 ± 0.02, respectively; within-population inbreeding coefficient of 0.089; and proportion of genetic variability accounted for by differences among breeds of 0.11 ± 0.01. Genetic differences were not significantly associated with the geographical location to which breeds were assigned or their country of origin. Still, the NeighborNet dendrogram depicted the clustering by geographic origin of several South American breeds (Criollo Boliviano, Criollo of northeastern Argentina wet, and Criollo of northeastern Argentina dry), but some unexpected results were also observed, such as the grouping of breeds from countries as distant as El Salvador, Mexico, Ecuador, and Cuba. The results of genetic structure and admixture analyses indicated that the most likely number of ancestral populations was 11, and most breeds clustered separately when this was the number of predefined populations, with the exception of some closely related breeds that shared the same cluster and others that were admixed. These results indicate that Criollo pigs represent important reservoirs of pig genetic diversity useful for local development as well as for the pig industry. © 2014 American Society of Animal Science. All rights reserved.
Gil J.,Institute Produccion Animal |
Theriogenology | Year: 2011
Two experiments were conducted to determine the effects of prostaglandin administration on ovarian follicular dynamics, conception, prolificacy, and fecundity in sheep. During the breeding season, multiparous Corriedale ewes were randomly allocated to two groups: 1) PG group (n = 15 and n = 135 in Experiments I and II, respectively): synchronized with two injections of DL-Cloprostenol (125 μg) given 7 d apart, and inseminated at a fixed time (Day 0), 48 h after the second injection; and 2) Control group (n = 15 and n = 73 in Experiments I and II): ewes in spontaneous estrus inseminated at detected estrus. Ewes received 100 10 6 sperm by intrauterine AI. Ultrasonography was used to evaluate growth of the ovulatory follicle, ovulation rate (OR), conception rate, and prolificacy on Days 30 and 60. Ewes from the group PG had a larger (4.8 0.5 mm, mean SEM; P < 0.05) ovulatory follicle that grew faster (1.2 0.3 mm/d, P = 0.08), and a lower OR (1.37 0.1, P < 0.05), compared to ewes from the Control group (3.9 0.2 mm, 0.7 0.2 mm/d, and 1.61 0.1 respectively). Plasma progesterone concentrations from Days -6 to 1 were lower in the PG group (P < 0.05), but plasma estradiol concentrations were similar between groups (P > 0.05). Progesterone concentrations were similar between groups during the early luteal phase and on Days 12 and 17 (P > 0.05). The embryo recovery rate (Day 7) tended to be lower in the PG group (39 vs 64%, P = 0.08), but embryo quality did not differ between groups. Conception, prolificacy and fecundity, were lower in the PG than in the Control group (P < 0.05). Cumulative reproductive losses were similar between groups, but more twins were lost in the PG group (P < 0.05). We concluded that in ewes synchronized with PGF 2α given twice, 7 d apart, lower reproductive performance was associated with an environment dominated by lower progesterone concentrations that stimulated the preovulatory follicle to grow faster and become larger; this was associated with lower rates of ovulation, conception, prolificacy, and fecundity. © 2011 Elsevier Inc.
Gruas C.,University of Zaragoza |
Llambi S.,Institute Produccion Animal |
Arruga M.V.,University of Zaragoza
Archives of Microbiology | Year: 2014
Legionella pneumophila is the primary cause of the legionellosis diseases (90 %) (Yu et al. in J Infect Dis 186:127-128, 2002; Doleans et al. in J Clin Microbiol 42:458-460, 2004; Den Boer et al. in Clin Microbiol Infect 14:459-466, 2008). In this study, methodologies based on molecular biology were developed in order to provide a quick diagnosis of the bacterial presence in water samples of Spain. Multiplex real-time polymerase chain reaction assays were realized to target the 16S rRNA and macrophage infectivity potentiator (mip) genes of, respectively, Legionella spp. and L. pneumophila including in the design of an internal control. The results obtained by the culture and the gene amplification methods agreed in 94.44 % for the 16S rRNA gene, and a concordance of 66.67 % of the cases was obtained for the mip gene. © 2013 Springer-Verlag Berlin Heidelberg.
De Basilio V.,Institute Produccion Animal |
Lovera M.,Institute Produccion Animal |
Tepper E.,Institute Produccion Animal |
Becerra A.,Institute Produccion Animal |
And 2 more authors.
Revista Cientifica de la Facultad de Ciencias Veterinarias de la Universidad del Zulia | Year: 2010
The effects of both food restriction (FR) and non food restriction (NFR) in Ross broilers under commercial conditions in Cagua, Venezuela, were evaluated. At 28d of age broilers, circular fences were placed in the shed and a total of 264 broilers, half males (M), half females (F) was randomly selected and allocated into two groups that underwent two experiments (EXP): Experiment 1 (EXP1): 120 broilers; Experiment 2 (EXP2): 144 broilers. The FR lasted 7h (9:00 to 16:00h) in both EXP. The following were measured: environmental temperature (ET); body temperature (BT), hyperventilation level (HL), food consumption (FC), weight gain (WG), food conversion index (FCI), and mortality. In EXP1, BT and HL were measured in an alternate way from 30 to 40d of age. In EXP2, they were measured in parallel between 29 and 38d of age. The average ET within the shed was 29.4°C (EXP1) y 30.9deg;C (EXP2), 1.4deg;C to 2deg;C higher than outside it. The FC was superior in M in both EXP, but the differences were lower among sexes in broilers under FR (EXP1), although non significant. No effect on WG and FCI was found in EXP2. In EXP2, the mortality reached 24.3% (35/144) at d 35 (alleged heat stress), with 75% more in FR than in NFR broilers; with a 50% more in M compared to F with FR. In EXP2, M had a higher (0.22deg;C) BT than F, being the most important differences in broilers with NFR food. In general, FR reduced HL in 19.8 insp/min in EXP1 and 12.3 insp/min in EXP2, respectively. The sex did not have any effect on HL in EXP2, although it was more important for F (29.1 insp/min) than for M (10.6 insp/min) in EXP1. The reduction in the risk of mortality due to heat stress was greater in F.