Coelho-Souza S.A.,Institute Ciencias do Mar Almirante Paulo Moreira IEAPM |
Coelho-Souza S.A.,Federal University of Rio de Janeiro |
Coelho-Souza S.A.,Institute Biofisica Carlos Chagas Filho |
Miranda M.R.,Federal University of Rio de Janeiro |
And 3 more authors.
Microbial Ecology | Year: 2013
The ecological interaction between microorganisms and seaweeds depends on the production of secondary compounds that can influence microbial diversity in the water column and the composition of reef environments. We adapted the 3H-leucine incorporation technique to measure bacterial activity in biofilms associated with the blades of the macroalgae Sargassum spp. We evaluated (1) if the epiphytic bacteria on the blades were more active in detritus or in the biofilm, (2) substrate saturation and linearity of 3H-leucine incorporation, (3) the influence of specific metabolic inhibitors during 3H-leucine incorporation under the presence or absence of natural and artificial light, and (4) the efficiency of radiolabeled protein extraction. Scanning electron microscopy showed heterogeneous distribution of bacteria, diatoms, and polymeric extracellular secretions. Active bacteria were present in both biofilm and detritus on the blades. The highest 3H-leucine incorporation was obtained when incubating blades not colonized by macroepibionts. Incubations done under field conditions reported higher 3H-leucine incorporation than in the laboratory. Light quality and sampling manipulation seemed to be the main factors behind this difference. The use of specific metabolic inhibitors confirmed that bacteria are the main group incorporating 3H-leucine but their association with primary production suggested a symbiotic relationship between bacteria, diatoms, and the seaweed. © 2012 Springer Science+Business Media, LLC.
Gevu K.V.,Federal Rural University of Rio de Janeiro |
Gevu K.V.,State University of Rio de Janeiro |
Da Cunha M.,State University of Norte Fluminense |
Barros C.F.,Institute Pesquisas Jardim Botanico Do Rio Of Janeiro Ipjbrj |
And 2 more authors.
Plant Systematics and Evolution | Year: 2014
Xylem structural characteristics are rarely used to separate tribes. This study aims to characterize the morphology of subterranean organs and determine both the occurrence and distribution of the vessel elements in ten species, belonging to Alpinieae and Zingibereae tribes. Species were from protected areas in the Brazilian Atlantic Rain Forest or from private cultivation areas. Roots and rhizomes processing followed both light and scanning electron microscopy procedures. According to morphological and anatomical characteristics, Euclidean distances among taxa were calculated, resulting in a dendrogram based on the unweighted pair group method with arithmetic mean (UPGMA) clustering. Despite our results indicating a similarity among all the species studied, they could be segregated by using qualitative and special metabolites characters in both the rhizome and the root. Vessel elements were found to be confined to the roots, presenting several basal characters in most of species, except for Alpinia purpurata and Etlingera species. © 2013 Springer-Verlag Wien.
Paradas W.C.,Institute Pesquisas Jardim Botanico Do Rio Of Janeiro Ipjbrj |
Andrade L.R.,Federal University of Rio de Janeiro |
Salgado L.T.,Institute Pesquisas Jardim Botanico Do Rio Of Janeiro Ipjbrj |
Collado-Vides L.,Florida International University |
And 2 more authors.
Algae | Year: 2015
The present study described with different microscopy approaches chloroplasts lobes in laurenciasensu latu (Rhodophyta) species and found inter-specific differences among them. Chloroplasts were investigated using confocal laser scanning microscopy (LSM), transmission electron microscopy (TEM) and high resolution scanning electron microscopy (HRSEM). Using and TEM and HRSEM images we distinguished chloroplasts with lobes than chloroplasts without lobes in Yuzurua poiteauivar. gemmifera(Harvey) M. J. Wynne and laurencia dendroideaJ. Agardh cortical cells. The LSM images showed chloroplasts lobes (CLs) with different morphologies, varying from thicker and longer undulated projections in Y. poiteauivar. and I. dendroideato very small and thin tubules as in laurencia translucidaFujii & Cordeiro-Marino. The diameter and length of CLs from Y. poiteauivar. and I. dendroideawere significantly higher than I. translucidaCLs (p < 0.01). Based on LSM observations, we suggest that lobes morphology has a taxonomic validity only to characterize I. translucidaspecies. © 2015 The Korean Society of Phycology.
De Lourdes Longo L.,Institute Pesquisas Jardim Botanico Do Rio Of Janeiro Ipjbrj |
Menezes Amado Filho G.,Laboratorio Of Macroalgas Marinhas
Historia, Ciencias, Saude - Manguinhos | Year: 2014
The ecosystems of Brazil’s continental shelf and oceanic islands comprise a variety of environments that display unique geomorphological and geophysical features and biotic components. The quest to attain knowledge of Brazilian marine fauna is hampered by coastline length, biodiversity, a high rate of endemism, and a shortage of specialized researchers. Based on a systematic bibliographic review, the article offers an overview of the history, current knowledge, and outlook for the field of marine biodiversity in Brazil. Our findings show that government initiatives have afforded greater knowledge of Brazilian marine fauna species and opened new perspectives, including reliance on complex tools to describe benthic marine habitats in terms of their geological, geophysical, and biotic composition. © 2014, Fundacao Oswaldo Cruz, All right reserved.