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Kosmann C.,University of Brasilia | Macedo M.P.,Institute Pesquisa Of Dna Forense | Barbosa T.A.F.,Primeira Delegacia Regional de Policia Civil Unai | Roberto J.R.P.,University of Brasilia
Revista Brasileira de Entomologia | Year: 2011

Chrysomya albiceps (Wiedemann) and Hemilucilia segmentaria (Fabricius) (Diptera, Calliphoridae) used to estimate the postmortem interval in a forensic case in Minas Gerais, Brazil. The corpse of a man was found in a Brazilian highland savanna (cerrado) in the state of Minas Gerais. Fly larvae were collected at the crime scene and arrived at the laboratory three days afterwards. From the eight pre-pupae, seven adults of Chrysomya albiceps (Wiedemann, 1819) emerged and, from the two larvae, two adults of Hemilucilia segmentaria (Fabricius, 1805) were obtained. As necrophagous insects use corpses as a feeding resource, their development rate can be used as a tool to estimate the postmortem interval. The post-embryonary development stage of the immature collected on the body was estimated as the difference between the total development time and the time required for them to become adults in the lab. The estimated age of the maggots from both species and the minimum postmortem interval were four days. This is the first time that H. segmentaria is used to estimate the postmortem interval in a forensic case. Source

Macedo M.P.,Institute Pesquisa Of Dna Forense | Macedo M.P.,University of Brasilia | Kosmann C.,University of Brasilia | Pujol-Luz J.R.,University of Brasilia
Revista Brasileira de Entomologia | Year: 2013

Origin of samples of Cannabis sativa through insect fragments associated with compacted hemp drug in South America. Insects associated with a seizure of Cannabis sativa L. may indicate the origin of the illicit drug. Nevertheless, no work regarding this subject has been previously published for South America. In the present investigation, seven kilograms of vegetal material (C. sativa) were inspected for insect fragments. Three species were identified and used to test the origin of the seizure of cannabis plant material: Euschistus heros (Fabricius, 1794), Thyanta perditor (Fabricius, 1794) (Heteroptera, Pentatomidae), and Cephalotes pusillus (Klug, 1824) (Hymenoptera, Formicidae). These insect species restricted the geographic origin of the drug to the Neotropical region, and their distribution patterns showed an overlap of the State of Mato Grosso (Brazil), Argentina, and Paraguay. Based on this information, two of the three major C. sativa growing areas in South America were excluded: (1) the Colombian territory and (2) northeastern Brazil. Source

Trindade-Filho A.,Institute Medicina Legal | Trindade-Filho A.,University of Brasilia | Ferreira S.,Institute Pesquisa Of Dna Forense | Oliveira S.F.,University of Brasilia
Genetics and Molecular Biology | Year: 2013

Deficiency paternity cases, characterized by the absence of the alleged father, are a challenge for forensic genetics. Here we present four cases with a female child and a deceased alleged father in which the analysis of a set of 21 or 22 autosomal STRs (AS STRs) produced results within a range of doubt when genotyping relatives of the alleged father. Aiming to increase the Paternity Index (PI) and obtain more reliable results, a set of 10 X-linked STR markers, developed by the Spanish and Portuguese Group of the International Society for Forensic Genetics (ISFG), was then added. Statistical analysis substantially shifted the results towards the alleged fatherhood in all four cases, with more dramatic changes when the supposed half-sister and respective mother were the relatives tested. © 2013, Sociedade Brasileira de Genética. Printed in Brazil. Source

Ferreira S.T.G.,Institute Pesquisa Of Dna Forense | Garrido R.G.,Institute Pesquisa e Pericias em Genetica Forense | Paula K.A.,Institute Pesquisa Of Dna Forense | Nogueira R.C.,Institute Medicina Legal | And 2 more authors.
Forensic Science International: Genetics Supplement Series | Year: 2013

In disaster victim identification (DVI), the quality and kind of source of samples along with the speed of sample collection and the preservation of samples play a decisive role in DNA typing and, thus, in the identification of the victims. In this study, we analyzed twenty cartilage samples from knees and twenty bone samples (phalanges from hallux) collected from the same victims of the biggest natural disaster in Brazil, in which there were 918 deaths. This incident occurred due to floods and mudslides in the mountainous region of the State of Rio de Janeiro in January 2011. The results of the amount of DNA recovered from the samples analyzed and the quality of the STRs profiles obtained demonstrated that cartilage and phalanges are excellent sources of samples for DNA typing in DVI. © 2013 Elsevier Ireland Ltd. Source

Ferreira S.T.G.,Forca Nacional | Ferreira S.T.G.,Institute Pesquisa Of Dna Forense | Kuser H.H.,Forca Nacional | Garrido R.G.,Institute Pesquisa e Pericias em Genetica Forense | And 4 more authors.
Forensic Science International: Genetics Supplement Series | Year: 2011

In mass fatality incidents there are critical variables that will shape the response to the events. These variables will determine different strategies of action and will require specific approaches for the appropriate disaster management and identification of victims. Magnitude and nature of the disaster, number of victims, if it is an open or closed event, degree of fragmentation and decomposition of bodies, accessibility of ante-mortem data, availability of DNA reference samples and kinds of post-mortem samples for DNA testing are some critical variables in disaster victim identification (DVI). In this study, we will discuss how some of these variables shaped the response and the results of the methods of identification by DNA, fingerprint and dental analysis in two different disasters that occurred in Brazil: Floods and mudslides in the mountainous region of the State of Rio de Janeiro, in January 2011, in which 895 people died, and a plane crash in the Brazilian Amazon rainforest, with 154 fatal victims, in September 2006. © 2011 Elsevier Ireland Ltd. Source

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