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Bottino C.G.,Institute Pesquisa e Pericias em Genetica Forense IPPGF | Bottino C.G.,National Institute of Metrology of Brazil | Chang C.W.,Thermo Fisher Scientific | Wootton S.,Thermo Fisher Scientific | And 6 more authors.
Forensic Science International: Genetics Supplement Series | Year: 2015

Massively parallel sequencing (MPS) platforms allow the simultaneous analysis of thousands to millions of DNA fragments, generating large amounts of data in a relatively short time frame compared with traditional sequencing methods. The aim of this study is to examine parameters for data interpretation generated for STR genotyping with a prototype 24-plex panel using Ion Torrent PGM. One male DNA reference sample was amplified with a 24-plex STR panel (kindly provided by Thermo Fisher Scientific) and sequenced in Ion Torrent PGM™. It was considered the numbers of alleles discriminated and the number of background reads from the number of total reads. Also parameters such as the percentage of stutter peaks, heterozygote peak high ratio (PHR) and sequence variants were evaluated. The full DNA profiles, with DNA input as low as 0.037. ng, corresponded to previous capillary electrophoresis (CE) results. Deep sequencing reads of 10,000 allowed the observation of intra-allelic variation on 5 loci. This study shows that MPS applied to STR genotyping improves accuracy and mixture interpretation. © 2015 Elsevier Ireland Ltd. Source


Brito F.C.A.,Faculdades Sao Jose | Brito F.C.A.,Institute Pesquisa e Pericias em Genetica Forense IPPGF | Prata D.R.B.M.,Institute Pesquisa e Pericias em Genetica Forense IPPGF | Martha S.F.P.,Institute Pesquisa e Pericias em Genetica Forense IPPGF | Bottino C.G.,Institute Pesquisa e Pericias em Genetica Forense IPPGF
Forensic Science International: Genetics Supplement Series | Year: 2015

Identification by DNA offers the ability to identify and compare individuals, including determining whether or not there is a genetic link between them. In events involving disaster victim identification (DVI), several biological samples can be used as source of DNA for genetic analyzes. However, many factors may affect the integrity of the genetic material obtained. The aim of this study was to verify the viability of using swabs from the inner mucosa of urinary bladder as a source of DNA to the identification of bodies without evident signs of decomposition by comparing two different methods of DNA extraction (organic versus Chelex® resin). Our results showed the viability of using swabs from the inner mucosa of the urinary bladder as DNA source of human identification, because besides showing reproducible and reliable results, this type of sample allows significant reductions in time and cost required for analysis. © 2015. Source

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