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Ferreira S.T.G.,Institute Pesquisa Of Dna Forense | Garrido R.G.,Institute Pesquisa e Pericias em Genetica Forense | Paula K.A.,Institute Pesquisa Of Dna Forense | Nogueira R.C.,Institute Medicina Legal | And 2 more authors.
Forensic Science International: Genetics Supplement Series | Year: 2013

In disaster victim identification (DVI), the quality and kind of source of samples along with the speed of sample collection and the preservation of samples play a decisive role in DNA typing and, thus, in the identification of the victims. In this study, we analyzed twenty cartilage samples from knees and twenty bone samples (phalanges from hallux) collected from the same victims of the biggest natural disaster in Brazil, in which there were 918 deaths. This incident occurred due to floods and mudslides in the mountainous region of the State of Rio de Janeiro in January 2011. The results of the amount of DNA recovered from the samples analyzed and the quality of the STRs profiles obtained demonstrated that cartilage and phalanges are excellent sources of samples for DNA typing in DVI. © 2013 Elsevier Ireland Ltd.


Ferreira S.T.G.,Forca Nacional | Ferreira S.T.G.,Institute Pesquisa Of Dna Forense | Kuser H.H.,Forca Nacional | Garrido R.G.,Institute Pesquisa e Pericias em Genetica Forense | And 4 more authors.
Forensic Science International: Genetics Supplement Series | Year: 2011

In mass fatality incidents there are critical variables that will shape the response to the events. These variables will determine different strategies of action and will require specific approaches for the appropriate disaster management and identification of victims. Magnitude and nature of the disaster, number of victims, if it is an open or closed event, degree of fragmentation and decomposition of bodies, accessibility of ante-mortem data, availability of DNA reference samples and kinds of post-mortem samples for DNA testing are some critical variables in disaster victim identification (DVI). In this study, we will discuss how some of these variables shaped the response and the results of the methods of identification by DNA, fingerprint and dental analysis in two different disasters that occurred in Brazil: Floods and mudslides in the mountainous region of the State of Rio de Janeiro, in January 2011, in which 895 people died, and a plane crash in the Brazilian Amazon rainforest, with 154 fatal victims, in September 2006. © 2011 Elsevier Ireland Ltd.


Netto M.S.C.,Institute Pesquisa e Pericias em Genetica Forense | Alves A.L.C.,Institute Pesquisa e Pericias em Genetica Forense | Carvalho E.F.,State University of Rio de Janeiro | Garrido R.G.,Institute Pesquisa e Pericias em Genetica Forense | Silva D.A.,State University of Rio de Janeiro
Forensic Science International: Genetics Supplement Series | Year: 2015

Forensic evidence from tissues embedded in paraffin archived for long periods and safeguarded in Pathology Institutes are, in some cases, the only sample available for DNA analysis. However, recovering DNA from this material can be challenging. The aim of this study is to evaluate methods of extraction and purification of DNA, using biological archival materials, in order to determine a protocol that is easy use and efficiency to adapt to the Institute of Research and Expertise in Forensic Genetics (IPPGF). Twenty-two samples from cadaver tissues included in paraffin blocks were submitted to two pre-treatments: deparaffinization using xylene and heating in microwave. After that, four methods were used for DNA extraction: phenol-chloroform-isoamyl alcohol (PCI), Chelex 100®, Purification on membranes (NucleoSpin) and the Resin purification method (DNA IQ). The quality and quantity of the DNA extracted was compared using RT-PCR. Additionally, PCR reactions were performed using the MiniFiler™ Kit. The methodology that presented better DNA recovery and more loci amplified in relation to the age of the tissues and also for the different tissues embedded in paraffin was PCI. These results reinforce and validate the maintenance of Standard Operating Procedure based on PCI used in IPPGF. © 2015 Elsevier Ireland Ltd.

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