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Córdoba, Argentina

Fernandez F.D.,Institute Patologia Vegetal | Conci V.C.,Institute Patologia Vegetal | Kirschbaum D.S.,Instituto Nacional de Tecnologia Agropecuaria | Conci L.R.,Institute Patologia Vegetal
European Journal of Plant Pathology | Year: 2013

Strawberry (Fragaria x ananassa Duch. cv. Camino Real) plants showing phyllody symptoms were detected in production fields in Bella Vista (Corrientes province, Argentina). DNA from all symptomatic samples analyzed yielded fragments of the expected size in PCR reactions using phytoplasma universal primers. DNA from asymptomatic plants yielded no products. The associated phytoplasma was designated as Argentinean Strawberry Phyllody (ASP). The ASP sequence of the 16S rRNA gene showed 99-98% homology with members of the 16SrVII ash yellows group. The putative RFLP profile was indistinguishable from the Argentinean alfalfa witches' broom (ArAWB) phytoplasma. The phylogenetic analysis of nearly full-length 16S rDNA sequence and 16S-23S spacer region yielded a consensus tree wherein ASP clustered into the ash yellows group (16Sr VII) with high confidence values (95), generating a separated branch (100 bootstrap value) together with ArAWB (16Sr VII-C). In Argentina, the 16Sr VII group was also detected in alfalfa (Medicago sativa L.) crops, and weeds [Artemisa annua L. and Conyza bonariensis (L.) Cronquist]. To our knowledge, this is the first report of a phytoplasma from the 16Sr VII group affecting strawberries in Argentina and worldwide. © 2012 KNPV. Source

Rodriguez Pardina P.E.,Institute Patologia Vegetal | Bejerman N.,Institute Patologia Vegetal | Luque A.V.,Institute Patologia Vegetal | Di Feo L.,Institute Patologia Vegetal
Virus Genes | Year: 2012

Sweet potato virus G belongs to the largest plant virus genus Potyvirus. This virus was detected for the first time in Argentina and then sequenced using the method of next-generation pyrosequencing. The complete genome was found to be 10,798 nucleotides excluding the poly-A tail with a predicted genome organization typical for a member of the genus Potyvirus. This is the first report of the complete genomic sequence of a SPVG isolated from South America. © 2012 Springer Science+Business Media, LLC. Source

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