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Miguel M.P.,Federal University of Goais | Sales T.P.,Federal University of Goais | de Menezes L.B.,Institute Patologia Tropical e Saude Publica IPTSP | Moraes J.M.,Federal University of Goais | And 3 more authors.
Ciencia Rural | Year: 2010

Mammary glands are the most usual sites of tumors in bitches, representing 25 to 30% of all types of cancer. During cancer genesis, several genetic changes alter the expression of different proteins like Cyr61, a peptide involved in cell proliferation and angiogenesis. This work aimed to determine through immunohistochemistry the expression profile of Cyr61 in normal and neoplastic mammary glands from bitches. So, 10 cases of each diagnose were selected: simple adenoma, complex carcinoma, simple solid carcinoma and fragments of normal mammary glands, in a total of 40 cases. Sections were subjected to immunohistochemistry, employing primary antibodies directed Cyr61. Cyr61 labeling of normal mammary epithelial cells is probably linked to its role in apoptosis and cellular proliferation. The labeling was more intense in normal and adenoma fragments and was discrete in the labeling malignant tumor sections, a different feature of expression from previously reports in human breast cancer. Immunohistochemical analysis with polyclonal antibody Cyr61 demonstrated the constitutive expression of the protein and contributed to neoplastic research alternative for tumors in bitches.

Luz C.,Institute Patologia Tropical e Saude Publica IPTSP | D'Alessandro W.B.,Institute Patologia Tropical e Saude Publica IPTSP | Rodrigues J.,Institute Patologia Tropical e Saude Publica IPTSP | Fernandes E.K.K.,Institute Patologia Tropical e Saude Publica IPTSP
Parasitology Research | Year: 2015

Conidia of the entomopathogenic fungus Metarhizium anisopliae (Ascomycota: Clavicipitaceae) were assessed against Rhipicephalus sanguineus (Arachnida: Ixodidae) eggs under laboratory conditions. Clusters of 25 eggs were applied either directly with the fungal conidial formulations or set on previously fungus-treated filter paper. Treatments consisted of conidia formulated in water or an oil-in-water emulsion at final concentrations of 3.3 × 103, 104, 3.3 × 104, 105, or 3.3 × 105 conidia/cm2. The development of mycelium and new conidia on egg clusters incubated at 25 °C and humidity close to saturation depended on conidial concentration, formulation, and application technique. No larvae eclosed from eggs after direct applications of conidia regardless of the formulation. The eclosion and survival of larvae from indirectly treated egg clusters depended on the type of formulation and conidial concentration applied. Oil-in-water formulations of conidia demonstrated the highest activity against eggs of R. sanguineus. © 2015 Springer-Verlag Berlin Heidelberg

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