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Djeddi S.,Annaba University | Karioti A.,University of Florence | Yannakopoulou E.,Institute of Physical Chemistry | Papadopoulos K.,Institute of Physical Chemistry | Chatter R.,Institute Pasteur Of Tunis 13
Records of Natural Products | Year: 2013

Part of this work deals with the isolation and structure elucidation of the main polar secondary metabolites of the aerial parts of Retama raetam (Forssk.) Webb & Berthel, as well as the evaluation of their potential analgesic properties, while the rest deals with the antioxidant activities of the aqueous extracts of roots, stem, fruits and flowers of the plant. It was found that the isoflavones genistein 1, 6-hydroxygenistein 2, 3'-Omethylorobol 3, pratensein 4, biochanin A 8, the flavones 6-hydroxyapigenin 7 and luteolin 5, the flavonol kaempferol 6, as well as the phenolic compound p-coumaric acid 9 reduce significantly the pain at a concentration dose of 1 mg/kg. The most active compounds were 3 and 8 (86.19% and 75.23%, respectively). The obtained aqueous extracts of R. raetam were also evaluated for their antioxidant activities using two different photometric methods; the results revealed that all extracts exerted very low free radical scavenging activity compared to the well-known butylated hydroxytoluene (BHT) and lower hydrogen peroxide blocking activity than positive control gallic acid.

Selmi S.,Laboratory of Aggression Physiology and Endocrine Metabolic Studies | Tounsi H.,Institute Pasteur Of Tunis 13 | Safra I.,Laboratory of Aggression Physiology and Endocrine Metabolic Studies | Abdellaoui A.,Laboratory of Aggression Physiology and Endocrine Metabolic Studies | And 3 more authors.
RSC Advances | Year: 2015

We aimed in the present work to evaluate the implication of oxidative stress in the toxicological effects of subchronic malathion exposure on reproductive function in mice. In this respect, we used prepubertal male mice separated into two groups: a control and a malathion treated group. Animals were treated by gavage (per orally, p.o.) with malathion at 200 mg kg-1, body weight (b.w.) during thirty days. We found that malathion treatment leads to the alteration of semen parameters such as a decrease of testosterone level and acetylcholinesterase activity, an induction of apoptosis and necrosis in spermatozoa as well as a decrease of reproductive performance of male mice. The histopathological examination showed a marked change in the testis tissue. Malathion intoxication was by an increase of malondialdehyde (MDA) level, a decrease of sulfhydril groups (-SH) content, as well as a depletion of antioxidant enzyme activities such as catalase (CAT), total superoxide dismutase (SOD), Cu/Zn-SOD and Mn-SOD in testis and epididymis. More importantly, malathion treatment clearly induced a decrease in mRNA expression of COX isoenzyme in cauda and epididymis as well as GPx-4 in testis and GPx-5 in epididymis. These data suggest that a marked deregulation of reproductive function in prepubertal male mice exposed to malathion might be partly due to pro-oxidant properties of the examined compound. This journal is © The Royal Society of Chemistry 2015.

Selectins belong to the family of adhesion molecules that recognize sugars as ligands through their Carbohydrate Recognition Domain (CRD). There are three types of selectin: the L-selectin (CD62L), which is constitutively expressed by most leukocyte populations, the P-selectin (CD62P) is found on activated platelets and endothelial cells, and the E-selectin (CD62E) expressed by activated endothelial cells. These three molecules exhibit high homology in their structures. Selectin-ligand interactions are among the most studied protein-glycan interactions in biology. The selectins and theirs ligands are involved in regulating inflammatory and immunological events that occur at the interface of the bloodstream and vessel walls. Their molecular partners are surface glycoconjugates harboring groups of the sialyl-Lewis antigens. This review presents an inventory of our current knowledge on the structures and functions of selectins and their ligands. We also provide an update on their involvement in pathophysiological processes, especially during inflammation and tumor development.

Ayari-Fakhfakh E.,Institute Pasteur Of Tunis 13 | Ghram A.,Institute Pasteur Of Tunis 13 | Bouattour A.,Institute Pasteur Of Tunis 13 | Larbi I.,Institute Pasteur Of Tunis 13 | And 6 more authors.
Veterinary Journal | Year: 2011

This study, carried out between September 2006 and January 2007, is the first cross-sectional serological investigation of peste-des-petits-ruminants (PPR) and Rift Valley fever (RVF) in Tunisia. The objective was to assess the potential need to develop a dual, recombinant PPR-RVF vaccine and how such a vaccine might be utilised in Tunisia. An overall PPR seroprevalence of 7.45% was determined, a finding supported by the high specificity (99.4%) and sensitivity (94.5%) of the ELISA used. On assessment of the diversity and density of mosquitoes in the sampling area, four species of RVF-vectors of the genus Aedes and Culex were identified. However, no serological evidence of RVF was found despite the use of a highly sensitive ELISA (99-100%). Larger scale investigations are underway to confirm these findings and the continuation of the emergency vaccination program against these two diseases remains valid. © 2010 Elsevier Ltd.

Rourou S.,Institute Pasteur Of Tunis 13 | Riahi N.,Institute Pasteur Of Tunis 13 | Majoul S.,Institute Pasteur Of Tunis 13 | Trabelsi K.,Institute Pasteur Of Tunis 13 | Kallel H.,Institute Pasteur Of Tunis 13
Applied Biochemistry and Biotechnology | Year: 2013

Subcultivation of Vero cells grown in a proprietary animal component-free medium named IPT-AFM, on microcarriers, was studied. TrypLE Select, a non-animal-derived protease, was used as an alternative to trypsin for cell passaging. We first studied the effect of increasing concentrations of TrypLE Select toward cell growth and then studied the inactivation of the protease using either soybean trypsin inhibitor (STI) or the soy hydrolysate Hypep 1510, in six-well plates. Data showed that cell growth was impaired by residual level of TrypLE Select; STI was identified as an efficient agent to neutralize this effect. To restore cell growth and inactivate TrypLE Select, STI should be added to the medium at least at 0.2 g L-1. Cells were also grown in spinner flask on 2 g L-1 Cytodex1 in IPT-AFM. In these conditions, the cell detachment yield was equal to 78 ± 8 %. Furthermore, cells exhibited a typical growth profile when using the dislodged cells to seed a new culture. A cell detachment yield of 70 ± 19 % was also achieved when the cells were grown in a 2-L stirred bioreactor in IPT-AFM, on 3 g L-1 Cytodex1. This protocol can be of great interest to scale-up the process of Vero cells cultivation in IPT-AFM on Cytodex1 from one stirred bioreactor culture to another. © 2013 Springer Science+Business Media New York.

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