Institute Pasteur dAlger

Algiers, Algeria

Institute Pasteur dAlger

Algiers, Algeria
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Salah R.,Polytechnic School of Algiers | Michaud P.,University Blaise Pascal | Mati F.,Mouloud Mammeri University | Harrat Z.,Institute Pasteur dAlger | And 3 more authors.
International Journal of Biological Macromolecules | Year: 2013

In the present study, anticancer activities of chitin, chitosan and low molecular weight chitin were evaluated using a human tumour cell line, THP-1. A molecular weight-activity relationship and an electrostatic interaction-activity relationship were determined. The cytotoxic effects of chitin and derivatives were also evaluated using a normal human foetal lung fibroblastic cell line, MRC-5 and the specific cytotoxicity of chitin and derivatives to tumour cell lines was demonstrated. The high antitumour effect of low molecular weight of chitin was established. © 2012 Elsevier B.V.


Kernif T.,Institute National dAgronomie | Kernif T.,Institute Pasteur dAlger | Aissi M.,Ecole Nationale Veterinaire dEl Harrach | Doumandji S.-E.,Institute National dAgronomie | And 3 more authors.
American Journal of Tropical Medicine and Hygiene | Year: 2010

Bartonella species are being recognized as important bacterial human and canine pathogens, and are associated with multiple arthropod vectors. Bartonella DNA extracted from blood samples was obtained from domestic dogs in Algiers, Algeria. Polymerase chain reaction (PCR) and DNA sequence analyses of the ftsZ gene and the 16S-23S intergenic spacer region (ITS) were performed. Three Bartonella species: Bartonella vinsonii subsp. berkhoffii, Bartonella clarridgeiae, and Bartonells elizabethae were detected infecting Algerian dogs. To our knowledge, this study is the first report of detection by PCR amplification of Bartonella in dogs in North Africa. Copyright © 2010 by The American Society of Tropical Medicine and Hygiene.


Mammeria A.B.,University dEl Tarf | Bitam I.,Institute Pasteur dAlger | Houhamdp M.,University Of Guelma
Bulletin de la Societe Zoologique de France | Year: 2012

Breeding pairs of the White Stork, Ciconia ciconia L. 1758, were monitored from 2007 to 2011 in the wetlands of El Tarf, northeastern Algeria, where they are numerous. The purpose was to define their demographic strategies and explain the functioning of populations, which had not been studied previously in this region. Nest locations were determined with a GPS receiver. The numbers of breeding pairs increased from 174 in 1996 to 475 in 2007 and to 634 in 2011; nest density increased from 25.22/100 km2 in 1996 to 64.76/100 km2 in 2011. These values vary within the area, with 61.42% of the breeding pairs being established in zone I (El Tarf and surroundings), and 35.57% in zone II (El Kala and surroundings). This variation is related to the extension of farmlands and to climatic conditions, which provide the species with good food resources, thus enhancing the survival rate of the fledglings and favouring sedentarily in some groups. In 2011,99% of the pairs produced fledglings, whereas in 1996 this proportion did not exceed 86% (MOALA GRINE, 1996). Changes related to local climatic conditions might constrain the development of this species.


Izri A.,Parasitologie Mycologie | Bitam I.,Institute Pasteur dAlger | Charrel R.N.,Institut Universitaire de France
Clinical Microbiology and Infection | Year: 2011

In August 2010, during an entomological programme targeting sandflies, in the region of Larbaa-Nath-Iraten, Wilaya of Tizi-Ouzou (Algeria), a female Aedes albopictus was trapped alive and partially engorged. To our knowledge, this is the first report of Ae. albopictus in Algeria and more widely in the Maghreb. © 2011 The Authors. Clinical Microbiology and Infection © 2011 European Society of Clinical Microbiology and Infectious Diseases.


Amraoui F.,Institute Pasteur du Maroc | Amraoui F.,Université Ibn Tofail | Krida G.,Tunis el Manar University | Krida G.,University of Carthage | And 8 more authors.
PLoS ONE | Year: 2012

West Nile fever (WNF) and Rift Valley fever (RVF) are emerging diseases causing epidemics outside their natural range of distribution. West Nile virus (WNV) circulates widely and harmlessly in the old world among birds as amplifying hosts, and horses and humans as accidental dead-end hosts. Rift Valley fever virus (RVFV) re-emerges periodically in Africa causing massive outbreaks. In the Maghreb, eco-climatic and entomologic conditions are favourable for WNV and RVFV emergence. Both viruses are transmitted by mosquitoes belonging to the Culex pipiens complex. We evaluated the ability of different populations of Cx. pipiens from North Africa to transmit WNV and the avirulent RVFV Clone 13 strain. Mosquitoes collected in Algeria, Morocco, and Tunisia during the summer 2010 were experimentally infected with WNV and RVFV Clone 13 strain at titers of 107.8 and 108.5 plaque forming units/mL, respectively. Disseminated infection and transmission rates were estimated 14-21 days following the exposure to the infectious blood-meal. We show that 14 days after exposure to WNV, all mosquito st developed a high disseminated infection and were able to excrete infectious saliva. However, only 69.2% of mosquito strains developed a disseminated infection with RVFV Clone 13 strain, and among them, 77.8% were able to deliver virus through saliva. Thus, Cx. pipiens from the Maghreb are efficient experimental vectors to transmit WNV and to a lesser extent, RVFV Clone 13 strain. The epidemiologic importance of our findings should be considered in the light of other parameters related to mosquito ecology and biology. © 2012 Amraoui et al.


Malek M.A.,Aix - Marseille University | Malek M.A.,University of Boumerdès | Hammani A.,Mouloud Mammeri University | Beneldjouzi A.,Institute Pasteur dAlger | Bitam I.,University of Boumerdès
New Microbes and New Infections | Year: 2015

In Algeria, PCR sequencing of pla, glpD and rpoB genes found Yersinia pestis in 18/237 (8%) rodents of five species, including Apodemus sylvaticus, previously undescribed as pestiferous; and disclosed three new plague foci. Multiple spacer typing confirmed a new Orientalis variant. Rodent survey should be reinforced in this country hosting reemerging plague. © 2014 The Authors.


PubMed | Aix - Marseille University, Institute Pasteur dAlger, University of Boumerdès and Mouloud Mammeri University
Type: | Journal: New microbes and new infections | Year: 2015

In Algeria, PCR sequencing of pla, glpD and rpoB genes found Yersinia pestis in 18/237 (8%) rodents of five species, including Apodemus sylvaticus, previously undescribed as pestiferous; and disclosed three new plague foci. Multiple spacer typing confirmed a new Orientalis variant. Rodent survey should be reinforced in this country hosting reemerging plague.


Aissi W.,Institute Pasteur Of Tunis | Ben Hellel K.,Service de pediatrie | Habboul Z.,Institute Pasteur Of Tunis | Ben Sghaier I.,Institute Pasteur Of Tunis | And 3 more authors.
Bulletin de la Societe de Pathologie Exotique | Year: 2015

Visceral leishmaniasis (VL) is an important health problem in Tunisia. It is most common in children under five years of age. The governorate of Kairouan (central Tunisia) is one of the most affected foci. The aim of this study was to update the epidemiological, clinical and biological features of the disease. The study concerned all VL cases admitted in the pediatric department of Kairouan hospital during 10 years (from 2004 to 2013). For every patient included in this study and when available, data such as sex, age, geographical origin and the condition of the patient at admission (clinical and biological findings) were collected. The myelogram results were also exploited as well as results of serology, culture, Real-Time polymerase chain reaction (PCR) and isoenzymatic typing of Leishmania isolates. Two hundred and forty cases were recorded. Rural cases (87.1%) were more prevalent than urban ones (12.9%). Age ranged from 2 months to 13 years (median, 18 months). The female/male sex ratio was 1.03. The diagnosis delays ranged from 1 day to 8 months (median, 15 days). The most common clinical symptoms at admission were splenomegaly (97.9%), fever (79.9%) and hepatomegaly (47.3%). The principal biological disturbances were anemia (91.7%), thrombocytopenia (83.9%) and leucopenia (56.1%). Among the different biological tools used for diagnosis confirmation, PCR was the most sensitive (100%). All 43 typed stocks corresponded to Leishmania (L.) infantum species. Although zymodeme MON-1 was predictably the most frequent (27 cases), L. infantum MON-24 and MON-80 were responsible of no negligible numbers of cases (11 and 5 cases respectively). The present study gave an updated epidemiological, clinical and biological profile of infantile VL in Tunisia. The diagnosis delays were considerably shortened compared to previous reports. However, an even earlier diagnosis of cases is needed to improve the disease prognosis. Real-Time PCR showed to be helpful in VL management. © 2015, Springer-Verlag France.


Berdjane-Brouk Z.,University of Paris 13 | Charrel R.N.,Institut Universitaire de France | Bitam I.,Institute Pasteur DAlger | Hamrioui B.,Laboratoire Of Parasitologie | Izri A.,University of Paris 13
Parasite | Year: 2011

We report for the first time the presence of Phlebotomus mascittii and the female of Phlebotomus chadlii in Algeria. These two species were collected during an entomological study conducted in endemic visceral leishmaniasis focus from the north part of the country, Kabylia.


PubMed | Institute Pasteur dAlger
Type: Journal Article | Journal: Ticks and tick-borne diseases | Year: 2012

We collected ticks from camels in 4 regions of southern Algeria (El Oued, Bechar, Ghardia, and Adrar) from February to October in 2008 and in April of 2011. A total of 307 ticks representing multiple species (including Hyalomma dromedarii, H. marginatum rufipes, H. impeltatum, and H. impressum), was tested for the presence of spotted fever group rickettsia DNA using gltA real-time quantitative PCR (qPCR). The presence of Rickettsia aeschlimannii was confirmed with a new qPCR using species-specific primers and Taqman probes based on the sca2 genes. The R. aeschlimannii sequence was further confirmed by detecting the gltA and outer membrane protein (ompA) genes in H. m. rufipes, H. impeltatum, and H. dromedarii ticks. These findings represent the first report of the detection of R. aeschlimannii in ticks collected from camels from southern Algeria.

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