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Algiers, Algeria

Aissi W.,Institute Pasteur Of Tunis | Ben Hellel K.,Service de pediatrie | Habboul Z.,Institute Pasteur Of Tunis | Ben Sghaier I.,Institute Pasteur Of Tunis | And 3 more authors.
Bulletin de la Societe de Pathologie Exotique | Year: 2015

Visceral leishmaniasis (VL) is an important health problem in Tunisia. It is most common in children under five years of age. The governorate of Kairouan (central Tunisia) is one of the most affected foci. The aim of this study was to update the epidemiological, clinical and biological features of the disease. The study concerned all VL cases admitted in the pediatric department of Kairouan hospital during 10 years (from 2004 to 2013). For every patient included in this study and when available, data such as sex, age, geographical origin and the condition of the patient at admission (clinical and biological findings) were collected. The myelogram results were also exploited as well as results of serology, culture, Real-Time polymerase chain reaction (PCR) and isoenzymatic typing of Leishmania isolates. Two hundred and forty cases were recorded. Rural cases (87.1%) were more prevalent than urban ones (12.9%). Age ranged from 2 months to 13 years (median, 18 months). The female/male sex ratio was 1.03. The diagnosis delays ranged from 1 day to 8 months (median, 15 days). The most common clinical symptoms at admission were splenomegaly (97.9%), fever (79.9%) and hepatomegaly (47.3%). The principal biological disturbances were anemia (91.7%), thrombocytopenia (83.9%) and leucopenia (56.1%). Among the different biological tools used for diagnosis confirmation, PCR was the most sensitive (100%). All 43 typed stocks corresponded to Leishmania (L.) infantum species. Although zymodeme MON-1 was predictably the most frequent (27 cases), L. infantum MON-24 and MON-80 were responsible of no negligible numbers of cases (11 and 5 cases respectively). The present study gave an updated epidemiological, clinical and biological profile of infantile VL in Tunisia. The diagnosis delays were considerably shortened compared to previous reports. However, an even earlier diagnosis of cases is needed to improve the disease prognosis. Real-Time PCR showed to be helpful in VL management. © 2015, Springer-Verlag France.


Mammeria A.B.,University dEl Tarf | Bitam I.,Institute Pasteur Dalger | Houhamdp M.,University of Guelma
Bulletin de la Societe Zoologique de France | Year: 2012

Breeding pairs of the White Stork, Ciconia ciconia L. 1758, were monitored from 2007 to 2011 in the wetlands of El Tarf, northeastern Algeria, where they are numerous. The purpose was to define their demographic strategies and explain the functioning of populations, which had not been studied previously in this region. Nest locations were determined with a GPS receiver. The numbers of breeding pairs increased from 174 in 1996 to 475 in 2007 and to 634 in 2011; nest density increased from 25.22/100 km2 in 1996 to 64.76/100 km2 in 2011. These values vary within the area, with 61.42% of the breeding pairs being established in zone I (El Tarf and surroundings), and 35.57% in zone II (El Kala and surroundings). This variation is related to the extension of farmlands and to climatic conditions, which provide the species with good food resources, thus enhancing the survival rate of the fledglings and favouring sedentarily in some groups. In 2011,99% of the pairs produced fledglings, whereas in 1996 this proportion did not exceed 86% (MOALA GRINE, 1996). Changes related to local climatic conditions might constrain the development of this species.


Salah R.,Polytechnic School of Algiers | Michaud P.,University Blaise Pascal | Mati F.,Mouloud Mammeri University | Harrat Z.,Institute Pasteur Dalger | And 3 more authors.
International Journal of Biological Macromolecules | Year: 2013

In the present study, anticancer activities of chitin, chitosan and low molecular weight chitin were evaluated using a human tumour cell line, THP-1. A molecular weight-activity relationship and an electrostatic interaction-activity relationship were determined. The cytotoxic effects of chitin and derivatives were also evaluated using a normal human foetal lung fibroblastic cell line, MRC-5 and the specific cytotoxicity of chitin and derivatives to tumour cell lines was demonstrated. The high antitumour effect of low molecular weight of chitin was established. © 2012 Elsevier B.V.


Malek M.A.,Aix - Marseille University | Malek M.A.,University of Boumerdes | Hammani A.,Mouloud Mammeri University | Beneldjouzi A.,Institute Pasteur Dalger | Bitam I.,University of Boumerdes
New Microbes and New Infections | Year: 2015

In Algeria, PCR sequencing of pla, glpD and rpoB genes found Yersinia pestis in 18/237 (8%) rodents of five species, including Apodemus sylvaticus, previously undescribed as pestiferous; and disclosed three new plague foci. Multiple spacer typing confirmed a new Orientalis variant. Rodent survey should be reinforced in this country hosting reemerging plague. © 2014 The Authors.


Kernif T.,Institute National dAgronomie | Kernif T.,Institute Pasteur Dalger | Aissi M.,Ecole Nationale Veterinaire dEl Harrach | Doumandji S.-E.,Institute National dAgronomie | And 3 more authors.
American Journal of Tropical Medicine and Hygiene | Year: 2010

Bartonella species are being recognized as important bacterial human and canine pathogens, and are associated with multiple arthropod vectors. Bartonella DNA extracted from blood samples was obtained from domestic dogs in Algiers, Algeria. Polymerase chain reaction (PCR) and DNA sequence analyses of the ftsZ gene and the 16S-23S intergenic spacer region (ITS) were performed. Three Bartonella species: Bartonella vinsonii subsp. berkhoffii, Bartonella clarridgeiae, and Bartonells elizabethae were detected infecting Algerian dogs. To our knowledge, this study is the first report of detection by PCR amplification of Bartonella in dogs in North Africa. Copyright © 2010 by The American Society of Tropical Medicine and Hygiene.

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