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Vietia D.,Institute Oncologia y Hematologia MPPS | Liuzzi J.,Servicio de Cabeza Y Cuello del Hospital Oncologico Padre Machado IVSS | Avila M.,Institute Oncologia y Hematologia MPPS | De Guglielmo Z.,Institute Oncologia y Hematologia MPPS | And 2 more authors.
ecancermedicalscience | Year: 2014

Introduction: Human Papillomavirus (HPV) has been associated with benign and malignant lesions in different epitheliums. The relationship between specific genotypes of high-risk HPV and some human cancers is well established. The aim of this work was to detect the HPV genotypes present in head and neck squamous cell carcinoma (HNSCC). Methods: We evaluated 71 samples of patients with histopathological diagnosis of HNSCC. The DNA extraction was conducted with the QIAGEN commercial kit. HPV detection and genotyping were performed by reverse hybridisation (INNO-LiPA) following the commercial specifications. Results: The mean age of the patients evaluated was 60.7 ± 13.11 years. The distribution of the lesions included 25 (35.20%) cases of squamous cell carcinoma (SCC) of the oral cavity, 23 (32.39%) of larynx, 16 (22.50%) of the oropharynx, 4 (5.63%) of paranasal sinus, and 2 (2.80%) cases of SCC of the nostril. Of the patients, 78.9% were males, and of these 76% were tobacco users and 67.6% were alcohol consumers. The viral DNA was detected in 67.6% of the samples. The oral cavity and the larynx were the highest HPV-positivity sites with 35.40% and 29.10% respectively. The most frequent genotype was 16 as single infection (18.70%), or in combination with another HPV types. In the oral cavity and larynx the genotypes 16 or the combination 6 and 51 were present in 11.76% and 14.28%, respectively; and in the oropharynx the most frequent genotype was 16 in 22.50% of the cases, and in the paranasal sinus 50% presented infection with HPV-6. We observed that tumours with most advanced size and stage presented greater HPV positivity. Conclusions: This study shows a high percentage of HPV positivity in SCC is mainly associated with high-risk HPV. It is important to highlight that viral infection, especially HPV-16, could be a risk factor in HNSCC progression. © the authors. Source


De Guglielmo Z.,Institute Oncologia y Hematologia MPPS | Avila M.,Institute Oncologia y Hematologia MPPS | Mora A.,Ginecoobstetras de la Maternidad Concepcion Palacios | Melendez M.,Ginecoobstetras de la Maternidad Concepcion Palacios | Correnti M.,Institute Oncologia y Hematologia MPPS
Revista de Obstetricia y Ginecologia de Venezuela | Year: 2013

Objective: In this study was conducted subclinical human papillomavirus detection in samples from patients diagnosed with cervical ectropion to assess the prevalence of human papillomavirus infection associated with this condition. Methods: DNA was extracted with organic solvents (phenol /chloroform- isoamylic). Human papillomavirus detection was performed by PCR with generic primers MY09 and MY11 and the viral typing was performed using a commercial MPCR kit. Setting: Laboratorio de Genetica Molecular-Instituto de Oncologia y Hematologia; Results: The results showed that 26% of the evaluated sample (13/50) was positive for the presence of human papillomavirus genome. Viral typing test identified high- oncogenic risk human papillomavirus (types 16 or 18) in 38.45.% of the positives cases. Likewise, 38.45% was low oncogenic risk (types 6, 11 or mixed infection with 6/11 human papillomavirus) and 23.07% could not be typified with the used methodology. Conclusions: Although this result is not statistically significant, the virus latent presence highlights the need for greater medical surveillance for positive patients, especially in cases where detected 16 and 18 high-risk oncogenic human papillomavirus types, because they may have increased risk of cervical cancer. Source


Veitia D.,Institute Oncologia y Hematologia MPPS | Liuzzi J.,Institute Oncologia y Hematologia MPPS | Correnti M.,Institute Oncologia y Hematologia MPPS | Avila M.,Institute Oncologia y Hematologia MPPS | And 3 more authors.
Revista Venezolana de Oncologia | Year: 2015

SUMMARY OBJECTIVE: The first and the most important cause of cancer in upper aero digestive tract are the smoking and the risk is proportional to the intensity of the exposure to tobacco. Researchers have detected the Epstein Barr Virus in squamous cell carcinoma of the oral mucosa, the hypopharynx and the larynx tumors has suggested a possible role of this virus in the development of malignancy in the aero digestive tract in this study pose the presence of Epstein Barr Virus genome and the assess their possible association with the development of the squamous cell carcinoma of head and neck. METHOD: We evaluated 50 biopsies of patients with histological diagnosis of head and cancer most were male patients with a mean age of 61.43 ± 13.71 years old. We performed the DNA extraction using chloroform: phenol with ethanol precipitation. RESULTS: The presence of EBV was detected by nested PCR amplification products were observed in agarose gels stained with 3[%] SYBER safe. In 44.[%] of the biopsies studied they showed the Epstein Barr virus genome, where more positive anatomical location was the oral cavity (40.90[%]), followed by the larynx and the oropharynx. CONCLUSIONS: It is important to note that 68.18.[%] of the positive cases were in advanced stages of disease that might suggest a greater presence of the viral genome in lesions that have progressed and have a poorer prognosis. Source


De Guglielmo Z.,Institute Oncologia y Hematologia MPPS | Veitia D.,Institute Oncologia y Hematologia MPPS | Avila M.,Institute Oncologia y Hematologia MPPS | Fernandes A.,Institute Oncologia y Hematologia MPPS | And 4 more authors.
Revista Venezolana de Oncologia | Year: 2015

OBJECTIVE: The human papilloma virus is the etiologic agent of the cervical cancer and has been linked as a risk factor in the development of the head and neck cancer. This malignancy has also been associated with Epstein Barr virus and the herpes simplex virus. With the herpes simplex virus, the results are controversial, especially in the studies based on molecular biology. The strongest evidence suggesting that herpes simplex virus as a risk factor or cofactor in the development of head and neck cancer and was obtained in the serological studies. METHOD: This study evaluated the presence, by PCR, of HSV 1 and HSV 2 in 45 samples of head and neck cancer who previously tested positive for the detection of HPV. RESULTS: We obtained 37.7[%] and 26.6[%] of the samples were positive for HSV 1 and HSV 2, respectively. CONCLUSION: Although no relationship was found between the infection with this virus and the other risk factors in terms of characteristics of the disease, its presence in a high percentage of the samples support the results reported in other molecular and serological studies, which suggest the herpes simplex virus as a risk factor or cofactor in the development of this malignancy. © 2015, Sociedad Venezolana de Oncologia. All rights reserved. Source

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