Yu N.,Xinjiang Institute of MeteriaMedica |
He C.-H.,Xinjiang Institute of MeteriaMedica |
Xing J.-G.,Xinjiang Institute of MeteriaMedica |
Zeng C.,Xinjiang Institute of MeteriaMedica |
Zhao J.,Xinjiang Institute of MeteriaMedica
Chinese Traditional and Herbal Drugs | Year: 2016
Objective: To investigate the technology for the separation and purification of extract in Dracocephalum moldevica (EDM) by macroporous resin. Methods: Static and dynamic adsorption-desorption were used to select the best one from seven different type macroporous resins; With the content of total flavonoids, tilianin, luteolin-7-O-β-D-glucuronide, and rosmarinic acid as indexes, the purification technology parameters of EDM were optimized. Results: HPD600 resin showed the best purifying profile, its optimum technology conditions were as follows: The optimum concentration of the sample liquid was 0.08 g/mL equivalent to raw material, the resin column diameter-height ratio was 1:9, the amount of used adsorption was 0.32 g dried medicinal herb/mL resin, sample flow rate was 1.5 BV/h, and adsorption time was 12 h. In the course of elution, the resin column chromatography was eluted with 6 BV of 70% ethanol after removing impurities with 4 BV of water by flow rate of 1.5 BV/h. The contents of total flavonoids, tilianin, luteolin-7-O-β-D-glucuronide, and rosmarinic acid were more than 53%, 5.5%, 4.7%, and 2.5%. Conclusion: Macroporous resin HPD600 is suitable to separate and purify EDM. © 2016, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.