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Munkhjargal T.,Obihiro University of Agriculture and Veterinary Medicine | Munkhjargal T.,Institute of Veterinary Medicine | Yokoyama N.,Obihiro University of Agriculture and Veterinary Medicine | Igarashi I.,Obihiro University of Agriculture and Veterinary Medicine
Parasitology Research | Year: 2016

Human babesiosis is the most important zoonotic protozoan infection in the world. This is the first report of the cloning, expression, purification, and immunobiochemical characterization of a methionine aminopeptidase 1 (MetAP1) protein from Babesia microti (B. microti). The gene encodes a MetAP1 protein of B. microti (BmMetAP1) of approximately 66.8 kDa that includes glutathione S-transferase (GST) tag and shows MetAP activity. BmMetAP1 was detected in a lysate of B. microti and further localized in cytoplasm of the B. microti merozoite. rBmMetAP1 was found to be immunogenic, eliciting a high antibody titer in mice. Moreover, rBmMetAP1 stimulated the production of IFN-γ and IL-12 but not IL-4. Finally, rBmMetAP1 was able to provide considerable protection to mice against a B. microti challenge infection based on a reduction in peak parasitemia levels and earlier clearance of the parasite as compared with control mice. Taken together, these results suggest that rBmMetAP1 confers significant protection against experimental B. microti infection and might be considered a potential vaccine target against human babesiosis. © 2016 Springer-Verlag Berlin Heidelberg

Vukovic Z.,Vinca Institute of Nuclear Sciences | Radenkovic M.,Vinca Institute of Nuclear Sciences | Stankovic S.J.,Vinca Institute of Nuclear Sciences | Vukovic D.,Institute of Veterinary Medicine
Journal of the Serbian Chemical Society | Year: 2011

The distribution and accumulation of assorted heavy metals and a long-lived radionuclide (Cu, Zn, Pb, Cd, U, Th and 137Cs) in the water and sediment of the River Sava (in Serbia) were investigated at three locations in the vicinity of industrial and urban settlements (Sabac, Obrenovac, Belgrade). The concentrations of heavy metals in the sediment were found to be in the ranges (mg kg-1): 29.6-145.1 for Cu, 53.2-253.6 for Zn, 14.2-78.6 for Pb, 0.3- -24.6 for Cd, and 4.0-12.5 Bq l -1 for 137Cs. These values correlate to the concentrations in the river water if expressed by equilibrium distribution coefficients Kd (dm 3 g -1) between the solid and liquid phases. The degrees of accumulation and enrichment of tracer metals were determined.

Brenig B.,Institute of Veterinary Medicine | Beck J.,Chronix Biomedical GmbH | Floren C.,Institute of Veterinary Medicine | Bornemann-Kolatzki K.,Chronix Biomedical GmbH | And 3 more authors.
Animal Genetics | Year: 2013

White Galloway cattle exhibit three different white coat colour phenotypes, that is, well marked, strongly marked and mismarked. However, mating of individuals with the preferred well or strongly marked phenotype also results in offspring with the undesired mismarked and/or even fully black coat colour. To elucidate the genetic background of the coat colour variations in White Galloway cattle, we analysed four coat colour relevant genes: mast/stem cell growth factor receptor (KIT), KIT ligand (KITLG), melanocortin 1 receptor (MC1R) and tyrosinase (TYR). Here, we show that the coat colour variations in White Galloway cattle and White Park cattle are caused by a KIT gene (chromosome 6) duplication and aberrant insertion on chromosome 29 (Cs29) as recently described for colour-sided Belgian Blue. Homozygous (Cs 29/Cs29) White Galloway cattle and White Park cattle exhibit the mismarked phenotype, whereas heterozygous (Cs29/wt 29) individuals are either well or strongly marked. In contrast, fully black individuals are characterised by the wild-type chromosome 29. As known for other cattle breeds, mutations in the MC1R gene determine the red colouring. Our data suggest that the white coat colour variations in White Galloway cattle and White Park cattle are caused by a dose-dependent effect based on the ploidy of aberrant insertions and inheritance of the KIT gene on chromosome 29. © 2013 The Authors, Animal Genetics © 2013 Stichting International Foundation for Animal Genetics.

Kameyama K.,Obihiro University of Agriculture and Veterinary Medicine | Nishimura M.,Obihiro University of Agriculture and Veterinary Medicine | Punsantsogvoo M.,Obihiro University of Agriculture and Veterinary Medicine | Punsantsogvoo M.,Institute of Veterinary Medicine | And 5 more authors.
Parasitology | Year: 2012

Neospora caninum is an intracellular parasite that poses a unique ability to infect a variety of cell types by causing host cell migration. Although previous studies demonstrated that parasite-derived proteins could trigger host cell migration, the related molecules have yet to be determined. Our study aimed to investigate the relationship between Neospora-derived molecules and host cell migration using recombinant protein of N. caninum cyclophilin (NcCyp). Indirect fluorescent antibody test revealed that NcCyp was expressed in the tachyzoite cytosol. Furthermore, NcCyp release from extracellular parasites was detected by sandwich enzyme-linked immunosorbent assay in a time-dependent manner. Recombinant NcCyp caused the cysteine-cysteine chemokine receptor 5-dependent migration of murine and bovine cells. Furthermore, immunohistochemistry indicated that NcCyp was consistently detected in tachyzoites distributed within or around the brain lesions. In conclusion, N. caninum-derived cyclophilin appears to contribute to host cell migration, thereby maintaining parasite/host interactions. © Copyright 2012 Cambridge University Press.

Munkhjargal T.,Obihiro University of Agriculture and Veterinary Medicine | AbouLaila M.,Obihiro University of Agriculture and Veterinary Medicine | AbouLaila M.,Menoufia University | Terkawi M.A.,Obihiro University of Agriculture and Veterinary Medicine | And 7 more authors.
American Journal of Tropical Medicine and Hygiene | Year: 2012

We evaluated the inhibitory effects of pepstatin A and mefloquine on the in vitro and in vivo growths of Babesia parasites. The in vitro growth of Babesia bovis, B. bigemina, B. caballi, and B. equi was significantly inhibited (P < 0.05) by micromolar concentrations of pepstatin A (50% inhibitory concentrations = 38.5, 36.5, 17.6, and 18.1 μM, respectively) and mefloquine (50% inhibitory concentrations = 59.7, 56.7, 20.7, and 4 μM, respectively). Furthermore, both reagents either alone at a concentration of 5 mg/kg or in combinations (2.5/2.5 and 5/5 mg/kg) for 10 days significantly inhibited the in vivo growth of B. microti in mice. Mefloquine treatment was highly effective and the combination treatments were less effective than other treatments. Therefore, mefloquine may antagonize the actions of pepstatin A against babesiosis and aspartic proteases may play an important role in the asexual growth cycle of Babesia parasites. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene.

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