Ye Y.X.,Institute of Vascular Biology |
Qin Z.,Institute of Vascular Biology |
Li J.C.,Institute of Vascular Biology |
Yuan Q.B.,Institute of Vascular Biology |
And 3 more authors.
Chinese Journal of Cancer Biotherapy | Year: 2014
Objective: To modify the classical chick embryo chorioallantoic membrane (CAM) xenograft model of tumuorigenesis and evaluate the effectiveness and feasibility of the modified model in the screening of anti-angiogenesis drugs. Methods: Fertilized chicken eggs randomized into two groups. Eggs in the classical model group were grafted with U87 human glioma cells and the anti-angiogenic activity of test drugs, thalidomide (50, 100, 200 μg/ml), G3B6 and DMSO (the control) was detemined by following the well-established classical methods. In the modified model group, the CAM was given an additional adaptation period of 9-16 h and then grafted with U8 cells by inoculating the cells into a silicone ring and then placing the ring on the half of the grade 1 vessel of the CAM. The test drugs were added into the silicone ring where their anti-angiogenic activity was evaluated. For both groups, the tumor morphology and the microvessel density (MVD) in the tumor tissue were examined under a stereo microscope with photos taken. Changes in the tumor histology was assessed by H-E staining and the expression of VEGFR2, the marker of the angiogenesis, was assessed by in situ hybridization. Results: The success rate of the xenograft was increased significantly without any negative effect on angiogenesis in the CAM in the modified model as compared with the classical model ([70 ± 4. 226]% vs [41. 25 ± 5. 154]%; t = 4. 314, P =0. 000 7). The tumor volume as also significantly increased in the modified model group as compared with thecalissical model group ([60. 20 ±6. 012] vs [15.97 ±2.403] mm3; t =6.012, P <0.000 1). Both thalidomide and G3B6 effectively inhibited the angiogenesis and reduced the MVD and VEGFR2 expression in the tumor tissue. Conclusion: The modified xenograft CAM model of tumuorigenesis developed in this study seems superior over the classical CAM model in studying tumorigenesis and screening anti-angiogenesis drugs.