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Wu X.Q.,Institute of Tuberculosis Research
Zhonghua nei ke za zhi [Chinese journal of internal medicine] | Year: 2012

To study the relationship between the genetic polymorphisms of carboxylesterase 1 gene (CES1) and the susceptibility to antituberculosis drug-induced hepatotoxicity (ATBDIH). Genetic polymorphisms of CES1 in 473 tuberculosis patients with or without hepatotoxicity (200:273) after antituberculosis chemotherapy were analyzed by PCR-MassArray. In 4 tags of CES1 single nucleotide polymorphism (SNP), the frequency of the rs1968753 allele had statistical difference between the hepatotoxicity group and the no-hepatotoxicity group(P = 0.0236). The characteristics of anti-hepatotoxicity had been shown relationship with rs8192950 (P = 0.044, OR = 0.649, 95%CI = 0.426 - 0.989, AC/AA) and rs1968753 (P = 0.048, OR = 0.556, 95%CI = 0.311 - 0.995, GG/AA). The diplotypes with 'CGC' haplotype exhibited significant protection against hepatotoxicity at one copy (P = 0.048, OR = 0.654, 95%CI = 0.430 - 0.996). The genetic polymorphisms of CES1 might have significant association with ATBDIH. SNP rs8192950 AC genotype and rs1968753 GG genotype might be the candidates for risk prediction of ATBDIH.

Guo H.B.,Northwest University, China | Cui X.M.,Wenshan Institute of Sanchi Ginseng | Cui X.M.,Yunnan University | An N.,Northwest University, China | Cai G.P.,Institute of Tuberculosis Research
Genetic Resources and Crop Evolution | Year: 2010

Sanchi ginseng (Panax notoginseng (Burkill) F. H. Chen) has been cultivated in China for more than 400 years, whose root is an important traditional Chinese medicine mainly used to stanch blood, to disperse gore and to reduce pain caused by injury due to falls. The cultivated populations of this crop are distributed in Wenshan mountain area of Yunnan with a narrow habitat, whose location is around N 23.5°, E 104° and altitude ranges from 1,200 to 2,000 m. Although its wild species has not been found, current cultivated populations show rich morphological variations in stem, leaf, root, flower and fruit. Recent studies exhibit that Sanchi root has more active compounds than the roots of P. ginseng and P. quinquefolius. Therefore, this crop needs further utilization. © Springer Science+Business Media B.V. 2010.

Liu Z.,Chongqing Medical University | Liu Z.,Institute of Tuberculosis Research | Xiao B.,Chongqing Medical University | Tang B.,Chongqing Medical University | And 12 more authors.
Microbes and Infection | Year: 2010

Helicobacter pylori (H. pylori) is a major human pathogenic bacterium in gastric mucosa. However, the regulatory mechanism of H. pylori-induced immune response is not clear. MicroRNAs (miRNAs) have recently emerged as key post-transcriptional regulators of gene expression, and their role in H. pylori infection is just beginning to be explored. Here, we first reported that H. pylori infection up-regulated the expression of miR-146a in gastric epithelial cells as well as in gastric mucosal tissues in NF-κB-dependent manner. In turn, miR-146a may downregulate the expression of target genes, interleukin-1 receptor-associated kinase 1 (IRAK1) and TNF receptor-associated factor 6 (TRAF6). Furthermore, miR-146a negatively regulated H. pylori-triggered interleukin (IL)-8, growth-related oncogene (GRO)-α, and macrophage inflammatory protein (MIP) -3α through diminishing NF-κB activity. In conclusion, H. pylori-induced miR-146a plays a potential role in a negative feedback loop to modulate the inflammation by targeting IRAK1 and TRAF6. © 2010 Institut Pasteur.

Song L.,Institute of Tuberculosis Research | Wu X.,Institute of Tuberculosis Research
International Journal of Antimicrobial Agents | Year: 2016

Resistance and tolerance to antituberculosis (anti-TB) drugs, especially the first-line drugs, has become a serious problem in anti-TB therapy. Efflux of antimicrobial agents via bacterial efflux pumps is one of the main reasons for drug resistance. Efflux pump inhibitors (EPIs) bind to efflux pumps to inhibit drug efflux and thus enhance the drug effect and reduce drug resistance. Studies on EPIs targeting the efflux pumps of Mycobacterium tuberculosis (Mtb) help to understand Mtb resistance and to identify the potential drug target and are of significance in guiding the development of new anti-TB drugs and optimal combinations. Currently, there are many potential EPIs under study, but none of them has been used clinically for anti-TB therapy. In this article, we will provide an overview on the current development of EPIs targeting the efflux pumps of Mtb and discuss their potential clinical applications. © 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

Zhang J.,Institute of Tuberculosis Research | Wu X.,Institute of Tuberculosis Research | Shi L.,CAS Institute of Biophysics | Liang Y.,Institute of Tuberculosis Research | And 5 more authors.
Clinica Chimica Acta | Year: 2012

Background: The diagnosis for smear-negative pulmonary tuberculosis (TB) is very difficult. Proteomic fingerprinting of sera is a potentially useful tool. Methods: This study analyzed the results of the proteomic fingerprinting in sera obtained from active TB patients and controls using the surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) and protein-chip technology. The peaks were detected and analyzed, and a diagnostic system was developed. The protein peak was identified using high performance liquid chromatography (HPLC)-tandem matrix-assisted laser desorption/ionization-TOF-MS (MALDI-TOF-MS). Results: Around 50 protein peaks were found significantly different between the TB patients and the controls (P<0.01). Three protein peaks at m/z 5643, 4486 and 4360 were selected for system classification and the development of a decision model. The model distinguished the TB patients from the controls with a sensitivity of 96.9% and a specificity of 97.8%, respectively. The diagnostic accuracy was up to 97.3%. The one most discrepant protein peak at m/z 5643 seen in sera of active TB patients was identified as orosomucoid. Conclusions: A diagnostic system for active TB was developed using mass spectrometry and protein chip technology and required only small-volume serum samples. One potential protein biomarker at m/z 5643 was identified as orosomucoid. © 2012.

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