Jin Z.Q.,Chinese Academy of Agricultural Sciences |
Jin Z.Q.,Institute of Tropical Bioscience and Biotechnology ITBB |
Xu B.Y.,Institute of Tropical Bioscience and Biotechnology ITBB |
Liu J.H.,Institute of Tropical Bioscience and Biotechnology ITBB |
And 2 more authors.
Acta Horticulturae | Year: 2011
The isolation of mRNA transcripts encoding proteins associated with physiological processes is a powerful tool for better understanding the mechanisms behind the processes. In a previous study, a forward suppression subtractive hybridisation (SSH) cDNA library was constructed to isolate differentially expressed genes at early stage of post-harvest banana ripening. SSH was performed with cDNA from banana fruit on the day of harvest as the "driver" and cDNA from banana fruit 2 days post harvest (DPH) as the "tester." A total of 289 clones in the SSH library were sequenced. BLASTX results revealed that 191 cDNAs had significant sequence homologies with known sequences in the NCBI database (2006). Of the 191 cDNAs, 138 were singletons and 53 belonged to divergent clusters containing 2-8 sequences. The identified cDNAs encoding proteins are involved in cellular processes such as metabolism, protein destination and storage, protein synthesis, signal transduction, transport and intracellular traffic, cell structure, growth and division, transcription and post-transcription, and disease and defence. To characterise differentially expressed cDNAs in the SSH library, cDNA microarray analysis was conducted. A total of 26 cDNAs in the 2-DPH banana fruit were found to be up-regulated, and these results were confirmed by using reverse transcriptase-polymerase chain reaction (RT-PCR). The involvement of some of the genes in fruit ripening needs to be further confirmed.