Institute of Transplantology and Artificial Organs

Moscow, Russia

Institute of Transplantology and Artificial Organs

Moscow, Russia
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Dolgikh M.S.,Institute of Transplantology and Artificial Organs | Livak D.N.,Institute of Transplantology and Artificial Organs | Krasheninnikov M.E.,Institute of Transplantology and Artificial Organs | Onishchenko N.A.,Institute of Transplantology and Artificial Organs
Biomeditsinskaya Khimiya | Year: 2011

The cultivation of multipotent mesenchymal stromal bone marrow cells and cells of A-431, MDCK, Vero, 3T3 and Hep-G2 was performed on polymeric films (PVA) with different hydrophobic fatty acid residues. The cells of different types grew on these films with different intensity, but in the most cases comparable with the cultivation control on usual plastic. The examined films were nontoxic to cells and sufficiently adhesive. They did not changed pH of cultural media, were optically transparent under microscope and comfortable in the experimental work. These films can be used as a model for the artificial organ construction. The covalent binding of different fatty acids to PVA shows possibility of the adaptable changes of films properties (hydrophobity and adhesiveness), and therefore possibility of the creation of optimal conditions for different cell types attachement and growth.


Dolgikh M.S.,Institute of Transplantology and Artificial Organs
Biomeditsinskaya Khimiya | Year: 2010

The liver transplantation is the most effective method for treating severe liver disease. The hepatocytes transplantation may serve as the perspective means for treating liver failure. This review analyzes the experimental approaches and perspectives on the adult hepatocytes use for the creation of implanting bioartificial liver module for hepatic failure treatment.


Dolgikh M.S.,Institute of Transplantology and Artificial Organs
Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry | Year: 2010

The liver transplantation is the most effective method for treating severe acute liver diseases. The hepatocytes transplantation may serve as the perspective means for treating liver failure. This review analyzes the experimental approaches and perspectives on the use of adult hepatocytes for the creation of implanting bioartificial liver module for treatment of hepatic failure. © 2010 Pleiades Publishing, Ltd.


Dolgikh M.S.,Institute of Transplantology and Artificial Organs | Livak D.N.,Institute of Transplantology and Artificial Organs | Krasheninnikov M.E.,Institute of Transplantology and Artificial Organs | Onishchenko N.A.,Institute of Transplantology and Artificial Organs
Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry | Year: 2011

The cultivation of multipotent mesenchymal stromal bone marrow cells (BM-MSC) and A-431, MDCK, Vero, 3T3 and Hep-G2 lines of cells was performed in DMEM medium using polyvinyl alcohol (PVA) polymer films as scaffolds. PVA was modified by covalent linking of various hydrophobic fatty acids. Different cell types exhibited different growth intensity on these films, which, however, in most cases was comparable with control cultivations on plastics. © 2011 Pleiades Publishing, Ltd.

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