Li F.-J.,Tianjin Polytechnic University |
Li F.-J.,Tianjin Union Institute of Biotechnology Development |
Feng J.,Tianjin Union Institute of Biotechnology Development |
Li S.,Chinese Academy of Sciences |
And 2 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2014
Background: Pasteurization is a perfect method for albumin virus inactivation, which may not be required for virus inactivation validation. However, there are no systematical reports concerning virus inactivation of hemoglobin blood substitutes. Objective: To explore the effects of pasteurization on the physicochemical properties and biological function of hemoglobin blood substitutes. METHODS: Appropriate cord blood samples were taken followed by centrifugation, washing blood, rupture of membranes, stabilizer treatment. In the control group, the samples were placed in 55 °C water bath, and when the temperature of hemoglobin solution reached (55±1) °C, a heat treatment began and lasted for 2 hours. In the pasteurization group, the samples were placed in 60 °C water bath, and when the temperature of hemoglobin solution reached (60±1) °C, a heat treatment began and lasted for 10 hours. The heating process was under continues nitrogen protection. Then, the hemoglobin solution was placed in ice bath and cooled to below 4 °C followed by low-speed centrifugation and filtration via microporous membrane, purification and viral inactivation thereby to obtain cord blood hemoglobin. Results And Conclusion: The products in the pasteurization group were all red clear liquid. There was no significant difference between the two groups in the yield, methemoglobin concentration, and oxygen-carrying capacity. The purification of the two groups was more than 98%. Two kinds of purification methods had no effects on the oxygen-carrying capacity of hemoglobin. Therefore, pasteurization method can replace thermosensitive purification method of 55 °C, 2 hours. The pasteurization method will not only ensure the physicochemical and biological properties of hemoglobin, but also achieve the purpose of virus inactivation.