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Guibout L.,Paris-Sorbonne University | Guibout L.,Institute Biophytis | Mamadalieva N.,Institute of the Chemistry of Plant Substances | Balducci C.,Institute Biophytis | And 2 more authors.
Phytochemical Analysis | Year: 2015

Introduction Ajuga turkestanica is a plant used in traditional medicine for its high ecdysteroid content, including the presence of the particularly active turkesterone, which possess efficient anabolic activity. Objectives To isolate and identify minor ecdysteroids present in a semi-purified plant fraction containing ca. 70% turkesterone. Material and Methods Multi-step preparative HPLC (combining RP- and NP-HPLC systems) was used to purify the different components present in the turkesterone fraction. Isolated compounds were identified by high-resolution mass spectrometry and 2D-NMR. Results Fourteen ecdysteroids (including turkesterone and 20-hydroxyecdysone) were isolated. Seven of these, all bearing an 11α-hydroxy group, were previously unreported. Conclusion Ajuga turkestanica ecdysteroids are characterised by the abundance of 11α-hydroxylated compounds and by the simultaneous presence of 24C, 27C, 28C and 29C ecdysteroids. It is expected that even more ecdysteroids are to be found in this plant since the starting material for this study lacked the less polar ecdysteroids. The simultaneous presence of 20-hydroxyecdysone and turkesterone (its 11α-hydroxy analogue) as the two major ecdysteroids suggests that every ecdysteroid is probably present in both 11α-hydroxy and 11-deoxy forms. Copyright © 2015 John Wiley & Sons, Ltd. Source


Mamadalieva N.Z.,Institute of the Chemistry of Plant Substances | Mamadalieva N.Z.,University of Tuscia | Mamedov N.A.,University of Massachusetts Amherst | Craker L.E.,University of Massachusetts Amherst | Tiezzi A.,University of Tuscia
Acta Horticulturae | Year: 2014

Of the more than 4500 species of higher plants that grow wild in Uzbekistan, over 100 species within the Lamiaceae family are native to the country. The cytotoxicity of 10 of these plant species was investigated for antiproliferative properties using methanol, hexane, chloroform, and water extracts. All tested water extracts of the plants exhibited moderate inhibition of cell proliferation with IC50 values above 200 μg/ml. The chloroform and hexane extracts showed more potent inhibition of cell proliferation on tested cells than that of the methanol and water extracts. The chloroform extract of Ajuga turkestanica, Phlomis bucharica, and Scutellaria ramosissima strongly inhibited cell growth in HeLa cells with IC50 values of 7.1, 26.3, and 23.2 μg/ml, respectively. The higher levels of cytotoxic activity were observed in chloroform and hexane extracts of A. turkestanica, Leonurus turkestanicus, P. bucharica, Phlomis thapsoides, S. ramosissima, and Stachys betonicflora to HL-60 cells. Both chloroform and hexane extracts of L. turkestanicus, P. bucharica, S. ramosissima, and Thymus seravschanicus displayed very pronounced activity towards P3X cell lines. Source


Mamadalieva N.Z.,Institute of the Chemistry of Plant Substances | Janibekov A.A.,Institute of the Chemistry of Plant Substances | Girault J.-P.,University of Paris Descartes | Lafont R.,University Pierre and Marie Curie
Natural Product Communications | Year: 2010

Chemical investigations of Silene viridiflora (L.) yielded a new ecdysteroid, 20-hydroxyecdysone 20,22-monoacetonide-25-acetate (1), and a known ecdysteroid, 2-deoxypolypodine B-3-β-D-glucoside (2). The elucidation of the chemical structures was established by 1D and 2D NMR experiments. Source

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